Large-scalein Vitro. Screening of Egyptian Native and Cultivated Plants for Schistosomicidal Activity

Abstract

In vitro bioassay screening of 346 methanol extracts originated from 281 native and cultivated plant species growing in Egypt, and related to 81 families, was carried out for schistosomicidal activity. The extracts were bioassayed at 100 mg=mL on viable Schistosoma mansoni mature worms in culture medium. Viability of worms was examined after exposure for 24 h, and mortality determined. Negative (DMSO) and positive (praziquantel) controls were used. Of the tested plant extracts, 72 were found to possess reproducible in vitro antischistosomal activity. These active extracts were further subjected to determination of their LC 50

Schistosomicidal and molluscicidal activities of two Junipers species cultivated in Egypt and the chemical composition of their essential oils

“…Two other plants grown in Egypt belonging to family Cupressaceae were screened in vivo for schistosomicidal activity, namely Chamaecyparis lawsoniane and Cupressus lasitanica Mill. The extract of the bark of the former plant proved to show considerable effect with an LC50=59.6 ppm (Yousif et al, 2007).…”

“…The schistosomicidal in vitro effect of each plant was determined according to Yousif et al (2007). The schistosome material used in the present study was supplied by the Schistosome Biological Supply Centre (SBSC) at Theodor Bilharz Research Institute, Cairo, Egypt.…”

Schistosomicidal and molluscicidal activities of aminoalkylamino substituted neo- and norneocryptolepine derivatives

“…The schistosomicidal bioassay used here followed the main procedure previously described by Yousif et al (2007) and Ramirez et al (2007).Thus, the parasite material was S. mansoni mature worms of two strains (Egyptian and Puerto Rican strains) maintained at the Schistosome Biological Supply Centre (SBSC), Theodor Bilharz Research Institute (TBRI), Cairo, Egypt. The mature worms were obtained from hamsters (Mesocricetus auratus) (Waterhouse, 1839) percutaneously infected with cercariae 7 weeks earlier.…”

In vitro antischistosomal activity of venom from the Egyptian snake Cerastes cerastes

“…Next, the worms were rapidly washed under sterile laminar fl ow conditions in Roswell Park Memorial Institute (RPMI) culture medium containing 20% fetal calf serum, 300mg streptomycin, 300U penicillin and 160µg gentamicin. Worms were then exposed to a range of sublethal concentrations of snake venom in sterilized tissue culture plates (28) .…”