“…Our protocol of directed multilineage differentiation assay for human adult NCSCs was described earlier in details [45]. Briefly, we used the following differentiation media and adhesive substrates: adipogenic differentiation medium: DMEM high glucose (4.5 g/l) (BioWest, France) supplemented with 10% FBS, 1 μ M dexamethasone, 50 μ M indomethacine, 250 μ M isobutylmethylxanthine, 5 μ g/ml insulin (all: Sigma-Aldrich, USA), and 5% horse serum (BioWest, France); osteogenic differentiation medium: DMEM low glucose (1.0 g/l) (BioWest, France) supplemented with 10% FBS, 100 nM dexamethasone, 10 mM β -glycerophosphate, and 50 μ g/ml ascorbate-2-phosphate (all: Sigma-Aldrich, USA); neuronal differentiation medium: neurobasal medium (Gibco, UK), 2% B27 supplement (Gibco, UK), 1% N2 supplement (Gibco, UK), 5 μ M ec32 synthetic retinoid (AMSBIO, UK), 1 μ M forskolin (Sigma-Aldrich, USA), 20 ng/ml NGF (PeproTech, USA), 20 ng/ml BDNF (PeproTech, USA), 20 ng/ml GDNF (PeproTech, USA), and 20 ng/ml IGF (Gibco, UK); glial differentiation medium: neurobasal (Gibco, UK) and DMEM:F12 (Gibco, UK) media in a ratio 1 : 1, 2% B27 supplement (Gibco, UK), 1% N2 supplement (Gibco, UK), 1 μ M ec32 synthetic retinoid (AMSBIO, UK), 5 μ M forskolin (Sigma-Aldrich, USA), 20 ng/ml Neuregulin-1 (Gibco, UK), 10 ng/ml PDGF-BB (PeproTech, USA), and 20 ng/ml IGF (Gibco, UK).…”