Large Molecular Forms of Cholecystokinin Circulating in Humans

Gaisano, Herbert Y.; Reilly, William M.; Go, Vay Liang W.; Olivero, Dennis K.; Miller, Laurence J.

doi:10.1097/00006676-198603000-00007

Cited by 7 publications

(1 citation statement)

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“…Prior studies (3,11,29) on the molecular forms of endocrine CCK involved formation of plasma as the first step in the processing of blood during the measurement of endocrine molecular forms and observed that CCK-33 was the major circulating form of CCK in humans. In addition, using similar methods that formed plasma as the first step in processing blood, others (9,18) have detected only CCK-8 or CCK-22 or both in rat plasma.…”

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Differential bile-pancreatic secretory effects of CCK-58 and CCK-8

Reeve

Wu²,

Keire³

et al. 2004

American Journal of Physiology-Gastrointestinal and Liver Physi

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In this work, we 1) synthesized rat CCK-58, 2) determined the amounts and forms of rat CCK in whole blood after stimulation of its release by casein, 3) determined the potency of CCK-8 and CCK-58 peptides to displace labeled CCK-8 from CCK(A) and CCK(B) receptors transfected into Chinese hamster ovary (CHO) cells, and 4) examined the biological actions of CCK-8 and rat CCK-58 in an anesthetized rat model. CCK-58 was the only detected endocrine form of CCK in rat blood. Synthetic rat CCK-58 was less potent than CCK-8 for displacing the label from CCK(A) and CCK(B) receptors in transfected CHO cells. However, rat CCK-58 was more potent than CCK-8 for stimulation of pancreatic protein secretion in the anesthetized rat. In addition, CCK-58 but not CCK-8 stimulated fluid secretion in this anesthetized rat model. These data suggest that regions outside the COOH terminus of rat CCK-58 influence the expression of CCK biological activity. The presence of only CCK-58 in the circulation and the fact that its biological activity differs from CCK-8 suggests that CCK-58 deserves scrutiny in other physiological models of CCK activity.

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confidence: 99%