Large isoform of MRJ (DNAJB6) reduces malignant activity of breast cancer

Mitra, Aparna; Fillmore, Rebecca A.; Metge, Brandon J.; Rajesh, Mathur; Xi, Yaguang; King, Judy; Ju, Jingfang; Pannell, Lewis K.; Shevde, Lalita A.; Samant, Rajeev S.

doi:10.1186/bcr1874

Cited by 99 publications

(116 citation statements)

References 45 publications

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“…To observe changes in the secreted protein profiles of melanoma samples following PAEP-silencing, mass spectral analysis was conducted on a Thermo Fisher Orbitrap mass spectrometer (Thermo Fisher, Waltham, MA, USA) with an Agilent 1200 Series Nanoflow LC (Agilent Technologies, Santa Clara, CA, USA) for sample introduction, as previously described (13)(14)(15). Prior to analysis, secreted proteins isolated from PAEP-knockdown and non-targeting knockdown 624.38-Mel cells were digested with trypsin in the presence of a reducing agent.…”

Section: Western Blot Analysismentioning

confidence: 99%

Optimization and establishment of RNA interference-mediated knockdown of the progestagen-associated endometrial protein gene in human metastatic melanoma cell lines

Chai¹,

Qiao²,

Wang³

et al. 2013

Molecular Medicine Reports

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Abstract. Progestagen-associated endometrial protein (PAEP), also termed glycodelin, is a 28-kDa glycoprotein of the lipocalin superfamily that is expressed in a variety of tumors, including gynecological tumors, lung cancer and melanoma. To determine the biological functions of the PAEP gene in tumor development and progression, the production of transient and stable PAEP knockdown cell models is required. In the present study, RNA interference technology was used to silence PAEP gene expression in melanoma and transfection was screened for and the conditions were optimized using fluorescence microscopy, flow cytometry, qPCR and western blot analysis. The results of the present study showed that the transient transfection of melanoma cells with 100 nmol/l PAEP siRNA or lentiviral PAEP small hairpin RNA (shRNA) [multiplicity of infection (MOI), 100 pfu/cell] efficiently knocked down target gene expression. To establish stable PAEP knockdown cell lines, melanoma cells were infected with a low MOI (10 pfu/cell) of lentiviral particles expressing PAEP shRNA. Following puromycin screening, the PAEP gene knockdown efficiency was demonstrated to be >80% in 624-Mel and 624.38-Mel cell lines, which was validated by western blot analysis. Therefore, melanoma cell lines with stable knockdown of PAEP were successfully established and may be used as effective cell models to study the biological functions of the PAEP gene in melanoma.

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Section: Western Blot Analysismentioning

confidence: 99%

Optimization and establishment of RNA interference-mediated knockdown of the progestagen-associated endometrial protein gene in human metastatic melanoma cell lines

Chai¹,

Qiao²,

Wang³

et al. 2013

Molecular Medicine Reports

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“…Interestingly, other studies have identified a role for Mrj in the regulation of cell proliferation (Mitra et al, 2008;Zhang et al, 2008;Dey et al, 2009). However, instead of promoting proliferation as demonstrated here, Mrj was required for cell cycle arrest (Mitra et al, 2008;Zhang et al, 2008).…”

Section: Discussionmentioning

confidence: 40%

Section: Discussionmentioning

confidence: 99%

“…However, instead of promoting proliferation as demonstrated here, Mrj was required for cell cycle arrest (Mitra et al, 2008;Zhang et al, 2008). For example, Mrj is expressed at a lower level in several breast cancer cell lines and overexpression of Mrj slowed tumor growth (Mitra et al, 2008). Zhang and colleagues found that overexpression of Mrj in HEK-293T cells promotes nuclear localization of Slfn1 (Schlafen1), which is necessary for cell cycle arrest in a cyclin D-dependent manner (Zhang et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

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Neural stem cell self‐renewal requires the Mrj co‐chaperone

Watson

Mattar

Schuurmans

et al. 2009

Developmental Dynamics

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The Mrj co-chaperone is expressed throughout the mouse conceptus, yet its requirement for placental development has prohibited a full understanding of its embryonic function. Here, we show that Mrj 2/2 embryos exhibit neural tube defects independent of the placenta phenotype, including exencephaly and thin-walled neural tubes. Molecular analyses revealed fewer proliferating cells and a down-regulation of early neural progenitor (Pax6, Olig2, Hes5) and neuronal (Nscl2, SCG10) cell markers in Mrj

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“…Targeted inhibition of Tid1-S may be a useful therapeutic tool in the management of MetRdependent malignancies [37] . MRJ (DNAJB6) was found to reduce tumorigenicity and metastasis of melanoma and breast cancer cells [41] . MRJ induces β-catenin degradation and may play a role in maintaining an epithelial phenotype [42] .…”

Section: Human Hsp40 and Cancermentioning

confidence: 99%

Tumour suppressor HLJ1: A potential diagnostic, preventive and therapeutic target in non-small cell lung cancer

Tsai¹

2014

WJCO

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Lung cancer is the leading cause of cancer-related mortality throughout the world. Non-small cell lung cancer (NSCLC) accounts for 85% of all diagnosed lung cancers. Despite considerable progress in the diagnosis and treatment of the disease, the overall 5-year survival rate of NSCLC patients remains lower than 15%. The most common causes of death in lung cancer patients are treatment failure and metastasis. Therefore, developing novel strategies that target both tumour growth and metastasis is an important and urgent mission for the next generation of anticancer therapy research. Heat shock proteins (HSPs), which are involved in the fundamental defence mechanism for maintaining cellular viability, are markedly activated during environmental or pathogenic stress. HSPs facilitate rapid cell division, metastasis, and the evasion of apoptosis in cancer development. These proteins are essential players in the development of cancer and are prime therapeutic targets. In this review, we focus on the current understanding of the molecular mechanisms responsible for HLJ1's role in lung cancer carcinogenesis and progression. HLJ1, a member of the human HSP 40 family, has been characterised as a tumour suppressor. Research studies have also reported that HLJ1 shows promising dual anticancer effects, inhibiting both tumour growth and metastasis in NSCLC. The accumulated evidence suggests that HLJ1 is a potential biomarker and treatment target for NSCLC.© 2014 Baishideng Publishing Group Inc. All rights reserved.Key words: Non-small cell lung cancer; Metastasis; HLJ1; Anticancer; Biomarker Core tip: HLJ1, a member of the human heat shock proteins 40 family, has been characterised as a tumour suppressor. Research studies have reported that HLJ1 shows promising dual anticancer effects, inhibiting both tumour growth and metastasis in non-small cell lung cancer (NSCLC). The accumulated evidence suggests that HLJ1 is a potential biomarker and treatment target for NSCLC. We propose a hypothetical model for the roles of HLJ1 stimulator in suppressing lung cancer tumourigenesis. Investigating the integrated and coordinated molecular mechanisms of HLJ1 may shed new light on the treatment of lung cancer. The development of drug targeting HLJ1 may be an effective approach for lung cancer therapy.Tsai MF, Wang CC, Chen JJW. Tumour suppressor HLJ1: A potential diagnostic, preventive and therapeutic target in nonsmall cell lung cancer.

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Large isoform of MRJ (DNAJB6) reduces malignant activity of breast cancer

Cited by 99 publications

References 45 publications

Optimization and establishment of RNA interference-mediated knockdown of the progestagen-associated endometrial protein gene in human metastatic melanoma cell lines

Optimization and establishment of RNA interference-mediated knockdown of the progestagen-associated endometrial protein gene in human metastatic melanoma cell lines

Neural stem cell self‐renewal requires the Mrj co‐chaperone

Tumour suppressor HLJ1: A potential diagnostic, preventive and therapeutic target in non-small cell lung cancer

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