Large differences in amino acid sequences among ferredoxins from several species of genus Solanum

Mino, Yoshiki; Hazama, Toshio; Machida, Yuji

doi:10.1016/s0031-9422(02)00485-5

Cited by 5 publications

(6 citation statements)

References 18 publications

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“…Previously, we have reported the primary structures of Fds from seven Datura plants [3][4][5][6] and other solanaceous plants [7][8][9][10][11] Our recent results have suggests that S. japonica is closely related taxonomically to Datura plants, and especially to D. arborea. Interestingly, both S. japonica and Datura plants contain tropane-alkaloids such as hyoscyamine, atropine, and scopolamine.…”

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Amino Acid Sequences of Ferredoxins from Atropa belladonna and Hyoscyamus niger: Their Similarities to Those in Other Tropane-Alkaloid-Containing Plants

Mino

Yukita

Hiratsuka

et al. 2005

Biological & Pharmaceutical Bulletin

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We have proposed the term 'protein chemotaxonomy' for molecular taxonomy based on the primary structures of common plant proteins, instead the of so-called secondary metabolites. To evaluate the effectiveness of this concept, we have carried out a series of studies on the family Solanaceae, using ferredoxin (Fd), an iron-sulfur electron-transfer protein.2) This protein was chosen for this study because it is easy to isolate and has an appropriate molecular weight for determining the primary structure. Previously, we have reported the primary structures of Fds from seven Datura plants [3][4][5][6] and other solanaceous plants 7-11) Our recent results have suggests that S. japonica is closely related taxonomically to Datura plants, and especially to D. arborea. Interestingly, both S. japonica and Datura plants contain tropane-alkaloids such as hyoscyamine, atropine, and scopolamine. It may be worthwhile to determine the relationship between pharmacologically important constituents and the amino acid sequences of Fds from these plants. These considerations led us to elucidate the amino acid sequences of Fds from Atropa belladonna and Hyoscyamus niger, which are important medicinal plants that contain tropane-alkaloids.In this report, we determined the primary structures of Fds from A. belladonna and H. niger (Solanaceae), and compared them with those of Fds from other higher plants as well as those from Datura plants and other solanaceous plants. MATERIALS AND METHODS Materials Atropa belladonna and Hyoscyamus nigerwere cultivated in the herb garden at Osaka University of Pharmaceutical Sciences.Isolation of Ferredoxin Each protein (15.4 or 3.0 mg) was purified from the fresh leaves (0.6 or 0.5 kg) of A. belladonna or H. niger as described previously. 3,7)Sequence Determination The amino acid sequences of the Fds were determined using a gas-phase protein sequencer with automated Edman degradation of S-carboxymethylcysteinyl (Cm) Fd and the peptides obtained by lysyl endopeptidase, trypsin, or endoproteinase Asp-N digestion. C-terminal analysis was carried out with carboxypeptidase Y.The detailed procedure and the other methods have been described previously. 3,7)Construction of a Phylogentic Tree A phylogenetic tree was constructed from the amino acid sequences (97 residues) of higher-plant Fds (30 species) using the unweighed pairgroup method with the arithmetical averages (UPGMA) method of Nei (GENETYX software, Software Development, Japan). 13) The molar absorption coefficient at 420 nm, based on the spectrum and protein determination, was 11000 M Ϫ1 cm Ϫ1 , which was similar to those of other higher-plant Fds.2,13) The biological activities and other physico-chemical properties of Ab-and HnFds will be published elsewhere, together with those of other solanaceous Fds.Sequence Determination The sequencing strategy is summarized in Fig. 1. The analytical results regarding the amino acid compositions of both Cm-Fds and the peptides obtained by enzymatic digestion, were consistent with the derived sequences. Automat...

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confidence: 89%

“…[3][4][5][6][9][10][11][15][16][17] The number of amino acid differences are 14-40 for different families and 0 to 4 for the same genus. [3][4][5][6]9) Although there only limited data are available concerning plants that belong to the same family, the sequences of several solanaceous Fds have been deter- mined.…”

Section: Propertiesmentioning

confidence: 99%

Amino Acid Sequences of Ferredoxins from Atropa belladonna and Hyoscyamus niger: Their Similarities to Those in Other Tropane-Alkaloid-Containing Plants

Mino

Yukita

Hiratsuka

et al. 2005

Biological & Pharmaceutical Bulletin

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“…2, the amino acid sequence is compared with those of higher-plant Fds. 1,[3][4][5][6][7][8][9][10][11][12][16][17][18] Compared with other Fds, a noticeable feature of this sequence is both an insertion of Tyr at the 3rd position from the amino terminus and a deletion of two amino acid residues at the carboxyl terminus. Compared with other higher-plant Fds, differences were observed at Leu-8 as with Leucaena glauca (Lg) (Leguminosae)-and Gleichenia japonica (Gj) (Filicales)-Fds, at Lys-14 as with Gj-Fd, at Tyr-73 as with Petroselinum sativum (Umbelliferae)-Fd, and at Ala-81 as with Medicago sativa (Leguminosae)-Fd.…”

Section: Propertiesmentioning

confidence: 99%

“…1,[3][4][5][6][7][8][9][10][11][12][16][17][18] The number of amino acid differences are 14-40 for different families and 0 to 4 for the same genus, except for the genus Solanam. [3][4][5][6][7][8][9][10][11][12]16) In our recent study, 2 to 19 amino acid differences were observed among different genera of Solanaceae; Datura, Physalis, Nicotiana, Capsicum, Scopolia, and Lycium. Table 1 shows amino acid differences in Pg-Fd compared to other higherplant Fds that have been determined so far.…”

Section: Propertiesmentioning

confidence: 99%

“…Previously, we reported the primary structures of Fds from 14 solanaceous plants and one leguminous plant. 1,[3][4][5][6][7][8][9][10][11][12] Our recent results have suggested that their amino acid sequences were related to their taxonomic position among plants that belong to the same genus or family, but not among plants in different families, although there may not be enough sequence data to reach any definite conclusions. It may be worthwhile to determine the amino acid sequences of Fds from many important medicinal plants that belong to diferent families.…”

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Protein Chemotaxonomy. XIII. Amino Acid Sequence of Ferredoxin from Panax ginseng

Mino

2006

Biological & Pharmaceutical Bulletin

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We have proposed the term 'protein chemotaxonomy' for molecular taxonomy based on the primary structures of common plant proteins, instead of so-called secondary metabolites. To evaluate the effectiveness of this concept, we carried out a series of studies on the family Solanaceae, using ferredoxin (Fd), an iron-sulfur electron-transfer protein.2) This protein was chosen for this study because it is easy to isolate and has an appropriate molecular weight for determining the primary structure. Previously, we reported the primary structures of Fds from 14 solanaceous plants and one leguminous plant.1,3-12) Our recent results have suggested that their amino acid sequences were related to their taxonomic position among plants that belong to the same genus or family, but not among plants in different families, although there may not be enough sequence data to reach any definite conclusions. It may be worthwhile to determine the amino acid sequences of Fds from many important medicinal plants that belong to diferent families. These considerations led us to elucidate the amino acid sequences of Fds from Panax ginseng (Araliaceae), the root of which is one of the most commonly used traditional medicines in China, Korea, and other Asian countries for the treatment of various diseases.In this report, we determined the primary structure of Fd from P. ginseng (Araliaceae), and compared it with those of Fds from other higher plants. MATERIALS AND METHODS MaterialsFresh leaves of Panax ginseng were kindly gifted by the Yatsuka Blanch of JA Kunibiki (Shimane Prefecture).Isolation of Ferredoxin The protein (4.2 mg) was purified from the fresh leaves (0.9 kg) of P. ginseng as described previously. 3,7)Sequence Determination The amino acid sequence of the Fd was determined using a gas-phase protein sequencer with automated Edman degradation of S-carboxymethylcysteinyl (Cm) Fd and the peptides obtained by lysyl endopeptidase, trypsin, or endoproteinase Asp-N digestion. The peptides were purified by reversed-phase HPLC using a m-Bondasphere C 18 -100 Å column (0.39ϫ15 cm, Waters) using the solvent system TFA-MeCN-H 2 O (Aϭ0.1% TFA, BϭMeCN containing 0.1% TFA) with a gradient program 0-40% B in 50 min, flow rate 1 ml min Ϫ1 . C-Terminal analysis was carried out with carboxypeptidase Y.The detailed procedure and the other methods have been described previously. 3,7)Construction of a Phylogenetic Tree A phylogenetic tree was constructed from the amino acid sequences (98 residues) of higher-plant Fds (34 species) using the unweighed pair-group method with the arithmetical averages (UPGMA) method of Nei (GENETYX software, Software Development, Japan). 13) RESULTS AND DISCUSSIONProperties The absorption maxima in the UV-Vis spectrum of P. ginseng (Pg)-Fd were at 275, 285 (sh), 330, 420, and 465 nm, and showed A max /A 275 nm ratios of 0.67, 0.47, and 0.41, respectively. This spectrum was characteristic of [2Fe-2S] Fds from other higher plants.14) The molar absorption coefficient at 420 nm, based on the spectrum and protein determination,...

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Solanum incanum and S. heteracanthum as sources of biologically active steroid glycosides: Confirmation of their synonymy

et al. 2012

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Large differences in amino acid sequences among ferredoxins from several species of genus Solanum

Cited by 5 publications

References 18 publications

Amino Acid Sequences of Ferredoxins from Atropa belladonna and Hyoscyamus niger: Their Similarities to Those in Other Tropane-Alkaloid-Containing Plants

Amino Acid Sequences of Ferredoxins from Atropa belladonna and Hyoscyamus niger: Their Similarities to Those in Other Tropane-Alkaloid-Containing Plants

Protein Chemotaxonomy. XIII. Amino Acid Sequence of Ferredoxin from Panax ginseng

Solanum incanum and S. heteracanthum as sources of biologically active steroid glycosides: Confirmation of their synonymy

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