Langmuir monolayer study of phospholipid DPPC on the titanium dioxide–chitosan–hyaluronic acid subphases

Abstract

The studies of model biological membranes consisted of phospholipid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were carried out by means of the Langmuir monolayer technique using subphases containing chitosan (Ch), titanium (IV) oxide (TiO 2 ), hyaluronic acid (HA) or mixture of them. The aim was to determine the effect of individual components of subphase and their respective combinations on behavior of the DPPC membrane. The systems were tested at room temperature (20 °C) and at a natural pH of about 4.8, which was close to the pH of the human skin (4.7-5.6). The surface pressure-area per molecule (π-A) isotherms were obtained. Their analysis showed that all substances studied affected the phospholipid membrane which was revealed in the changes of mean molecular area, compression modulus and pressure of the liquidexpanded/liquid-condensed (LE/LC) phase transition. The results were discussed in terms of the nature and strength of mutual interactions. The more profound effect was found at low surface pressures at which the monolayers occurred in more expanded state. However, at the surface pressure corresponding to that of biological membranes, systems had very similar parameters compared to model DPPC isotherm.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Wettability of DPPC Monolayers Deposited from the Titanium Dioxide–Chitosan–Hyaluronic Acid Subphases on Glass

“…However, to choose which surfaces, hydrophobic or hydrophilic, are more appropriate for the extensive biological studies involving cell differentiation in the human skin in vitro studies are necessary. Based on our present and previous results [17,19,26,29] we think that the moderate hydrophilic surfaces will be the most useful for skin substitution. Also as reported by Rivero et al [30] the moderate hydrophilic surfaces are favorable for cell adhesion and proliferation.…”

“…This can suggest the penetration of nanoparticle TiO 2 between the DPPC molecules in the monolayer which may further be accessible to the formamide droplets of, causing them to spread over the 'plate' surface. The fact of oxide particles migration into the lipid film was also observed during the analysis of surface pressure isotherms as a function of the area per molecule (π-A) which was described in detail in our last paper [26].…”

“…• , suggesting the existence of weak interactions with water. In the first case (glass/AA/DPPC), the monolayer was highly packed [26]. It is well-known that the more condensed monolayer is, the less permeable to water molecules and other liquids.…”

“…Only in the case of glass/AA/HA/DPPC → glass/AA/HA/TiO 2 /DPPC there was a decrease of this parameter (27.3 • ) after the addition of TiO 2 . This may be related to the slight loosening of the monolayer structure at the transfer pressure after the inorganic substance addition to the subphase [26] which makes it more permeable than glass/AA/HA/DPPC. As a result, water molecules may have access to and interact with subphase components.…”

Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity

“…Thus, it can be assumed that the lipid monolayers, obtained by the Langmuir technique, mimic the outer surface of the native membrane of the cell [ 30 ].…”