Landscape of SARS-CoV-2 spike protein-interacting cells in human tissues

Zheng, Bo; Yuan, Manman; Ma, Qiang; Wang, Shenglan; Tan, Yang; Xu, Yizhu; Ye, Jing; Gao, Yanjie; Sun, Xun; Yang, Zifeng; Xu, Peipei; Kong, Ling-Dong; Wu, Xingxin; Xu, Qiang

doi:10.1016/j.intimp.2021.107567

Cited by 16 publications

(9 citation statements)

References 36 publications

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“…Importantly, previous studies have shown that COVID-19 can significantly affect the hematological and immunological systems, resulting in lymphopenia, thrombocytopenia, immature/dysfunctional neutrophil accumulation, etc., suggesting dysregulated hematopoiesis in the BM of COVID-19 patients. Using RBD subdomain 1 of the spike protein of SARS-CoV-2 (RBD-SD1) as a probe to investigate the potential tropism of SARS-CoV-2 in 33 types of normal human tissue, RBD-SD1 has been found to strongly interact with BM cells [ 145 ]. We recently conducted a scRNA-seq analysis (Wang et al, Cell Discovery, accepted) to characterize the BM mononuclear cell atlas in COVID-19 patients.…”

Section: Immune Landscape Of Covid-19mentioning

confidence: 99%

Multiomics: unraveling the panoramic landscapes of SARS-CoV-2 infection

Wang

Liu

et al. 2021

Cell Mol Immunol

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In response to emerging infectious diseases, such as the recent pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is critical to quickly identify and understand responsible pathogens, risk factors, host immune responses, and pathogenic mechanisms at both the molecular and cellular levels. The recent development of multiomic technologies, including genomics, proteomics, metabolomics, and single-cell transcriptomics, has enabled a fast and panoramic grasp of the pathogen and the disease. Here, we systematically reviewed the major advances in the virology, immunology, and pathogenic mechanisms of SARS-CoV-2 infection that have been achieved via multiomic technologies. Based on well-established cohorts, omics-based methods can greatly enhance the mechanistic understanding of diseases, contributing to the development of new diagnostics, drugs, and vaccines for emerging infectious diseases, such as COVID-19.

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Section: Immune Landscape Of Covid-19mentioning

confidence: 99%

Multiomics: unraveling the panoramic landscapes of SARS-CoV-2 infection

Wang

Liu

et al. 2021

Cell Mol Immunol

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“…Peripheral immune cells are derived from hematopoietic stem and progenitor cells (HSPCs) in bone marrow (BM) 25 . In a recent report, recombinant receptor-binding subdomain 1 of spike protein of SARS-CoV-2 (RBD-SD1) was used as probe to study the potential tropism of SARS-CoV-2 in 33 types of normal human tissues, and the results showed that over 80% of RBD-SD1 probe strongly interacted with BM cells 26 , suggesting the importance of understanding how COVID-19 affects the BM niche, especially the hematopoietic stem cell (HSC) 27 .…”

Section: Introductionmentioning

confidence: 99%

Dysregulated hematopoiesis in bone marrow marks severe COVID-19

et al. 2021

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Severe coronavirus disease 2019 (COVID-19) is often indicated by lymphopenia and increased myelopoiesis; however, the underlying mechanism is still unclear, especially the alteration of hematopoiesis. It is important to explore to what extent and how hematopoietic stem cells contribute to the impairment of peripheral lymphoid and myeloid compartments in COVID-19 patients. In this study, we used single-cell RNA sequencing to assess bone marrow mononuclear cells from COVID-19 patients with peripheral blood mononuclear cells as control. The results showed that the hematopoietic stem cells in these patients were mainly in the G1 phase and prone to apoptosis, with immune activation and anti-viral responses. Importantly, a significant accumulation of immature myeloid progenitors and a dramatic reduction of lymphoid progenitors in severe cases were identified, along with the up-regulation of transcription factors (such as SPI1, LMO4, ETS2, FLI1, and GATA2) that are important for the hematopoietic stem cell or multipotent progenitor to differentiate into downstream progenitors. Our results indicate a dysregulated hematopoiesis in patients with severe COVID-19.

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“…SARS-CoV-2 was reported to invade various tissues such as the lung, nerve, adrenal, esophagus, thymus, pancreas, breast, skin cervix, and lymph node ( 22 ), with different susceptibility across these tissues ( 22 , 23 ). In this study, we analyzed the expression profiles of SARS-CoV-2-required genes in normal tissue types and explored whether this might influence the susceptibility of the corresponding tissue tumor to SARS-CoV-2.…”

Section: Resultsmentioning

confidence: 99%

The Potential Mechanism of Cancer Patients Appearing More Vulnerable to SARS-CoV-2 and Poor Outcomes: A Pan-Cancer Bioinformatics Analysis

et al. 2022

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To explore the potential mechanism of cancer patients appearing more vulnerable to SARS-CoV-2 infection and poor COVID-19 outcomes, we conducted an integrative bioinformatics analysis for SARS-CoV-2-required genes and host genes and variants related to SARS-CoV-2 susceptibility and COVID-19 severity. BLCA, HNSC, KIRC, KIRP, LGG, PCPG, PRAD, TGCT, and THCA patients carrying rs10774671-A (OAS1) genotype may be more likely to have poor COVID-19 outcomes relative to those who carry rs10774671-G, because individuals carrying rs10774671-A will have lower expression of OAS1, which serves as a protective factor against SARS-CoV-2 processes and poor COVID-19 outcomes. SARS-CoV-2-required genes were correlated with TME, immune infiltration, overall survival, and anti-cancer drug sensitivity. CHOL patients may have a higher risk of SARS-CoV-2 infection than healthy subjects. SARS-CoV-2-induced ACE2 and NPC1 elevation may have a negative influence on the immune responses of LUSC and CD8+T infiltration of LUAD, and negatively affect the sensitivity of anti-lung cancer drugs. LUSC and LUAD patients may have a varying degree of adverse outcomes if they are infected with SARS-CoV-2. miR-760 may target and inhibit ACE2 expression. Cancer patients appearing vulnerable to SARS-CoV-2 infection and having poor COVID-19 outcomes may be partly due to host genetic factors and dysregulation of SARS-CoV-2-required genes. OAS1, ACE2, and miR-760 could serve as the treatment and intervention targets for SARS-CoV-2.

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Landscape of SARS-CoV-2 spike protein-interacting cells in human tissues

Cited by 16 publications

References 36 publications

Multiomics: unraveling the panoramic landscapes of SARS-CoV-2 infection

Multiomics: unraveling the panoramic landscapes of SARS-CoV-2 infection

Dysregulated hematopoiesis in bone marrow marks severe COVID-19

The Potential Mechanism of Cancer Patients Appearing More Vulnerable to SARS-CoV-2 and Poor Outcomes: A Pan-Cancer Bioinformatics Analysis

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