LAMP assay to detect Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in shrimp

Kongrueng, Jetnaphang; Tansila, Natta; Mitraparp-Arthorn, Pimonsri; Nishibuchi, Mitsuaki; Vora, Gary J.; Vuddhakul, Varaporn

doi:10.1007/s10499-014-9874-3

Cited by 44 publications

(34 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Otros estudios realizados mediante ensayos con la tecnología LAMP, han desarrollado y validado primers LAMP-A y LAMP-B, para identificar específicamente cepas de V. parahaemolyticus causantes de AHPND (Kongrueng et al, 2015). Además, se han desarrollado métodos cuantitativos (qPCR), basados en una sonda TaqMan, con el fin de cuantificar los plásmidos presentes en V. parahaemolyticus, el cual genera un amplicón de 135 pb que se produce a partir de la secuencia de Pir A (Han et al, 2015c).…”

Section: Técnicas Moleculares Primers Utilizados Y Sus Particularidadesunclassified

Necrosis aguda del hepatopáncreas: una revisión de la enfermedad en Penaeus vannamei

Mejías

Peña²,

Aranguren

2017

Mesoam. Agron.

View full text Add to dashboard Cite

Section: Técnicas Moleculares Primers Utilizados Y Sus Particularidadesunclassified

Necrosis aguda del hepatopáncreas: una revisión de la enfermedad en Penaeus vannamei

Mejías

Peña²,

Aranguren

2017

Mesoam. Agron.

View full text Add to dashboard Cite

“…; Kongrueng et al . ). The effect of PirA‐like toxin administration by bath immersion on mortality after V. parahaemolyticus AHPND experimental challenge is shown in Table .…”

Section: Effect Of Recombinant Pira‐like Toxin Administration By Bathmentioning

confidence: 97%

“…The acute hepatopancreatic necrosis disease (AHPND) is a serious concern in shrimp industry worldwide since 2009 causing a serious economic impact; therefore, strategies to control and treat AHPND are urgently needed (Kongrueng et al . ; Boonyawiwat et al . ; Enríquez‐Espinoza et al .…”

Section: Effect Of Recombinant Pira‐like Toxin Administration By Bathmentioning

confidence: 99%

Recombinant PirA‐like toxin protects shrimp against challenge with Vibrio parahaemolyticus, the aetiological agent of acute hepatopancreatic necrosis disease

Campa‐Córdova¹,

León-Gallo

Romero-Maldonado

et al. 2017

Journal of Fish Diseases

View full text Add to dashboard Cite

“…AHPND Vp designated as PSU 5429, PSU 5499, PSU 5562, and PSU 5579 were isolated from AHPNDafflicted shrimp collected from various farms in southern Thailand (Kongrueng et al 2015) and were used as mixed prey bacteria to isolate BALOs. AHPND Vp PSU 5429 was used as a host prey for other assays throughout this study.…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Isolation of Bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Kongrueng¹,

Mitraparp-Arthorn²,

Bangpanwimon³

et al. 2017

Dis. Aquat. Org.

View full text Add to dashboard Cite

Acute hepatopancreatic necrosis disease, a severe disease of shrimp, is caused by Vibrio parahaemolyticus (AHPND Vp), a halophilic bacterium harboring a plasmid that contains toxin genes homologous to Photorhabdus insect-related toxins. We obtained 9 isolates of Bdellovibrio and like organisms (BALOs) from water and sediment samples in Thailand. Using 16S rRNA sequencing, all of the organisms were identified as Bacteriovorax spp. and were able to attack all tested AHPND Vp isolates. In addition, their various susceptible hosts, including Gram-positive and Gram-negative bacteria, were observed. The optimal ratio for interaction between the Bacteriovorax isolate BV-A and AHPND Vp was determined to be 1:10. The suitable conditions applied for co-culture between BV-A and AHPND Vp were 30°C, 2% NaCl, and pH 7.6. The capability of BV-A to reduce numbers of AHPND Vp in vitro was observed in co-culture after incubation for 2 d and continued until the end of the incubation period. In vivo, BV-A was able to reduce mortality of shrimp post-larvae infected with AHPND Vp. In addition, BV-A significantly decreased the formation of biofilm by AHPND Vp. These findings provide evidence for using Bacteriovorax as a biocontrol of AHPND Vp in shrimp aquaculture.

show abstract

LAMP assay to detect Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in shrimp

Cited by 44 publications

References 15 publications

Necrosis aguda del hepatopáncreas: una revisión de la enfermedad en Penaeus vannamei

Necrosis aguda del hepatopáncreas: una revisión de la enfermedad en Penaeus vannamei

Recombinant PirA‐like toxin protects shrimp against challenge with Vibrio parahaemolyticus, the aetiological agent of acute hepatopancreatic necrosis disease

Isolation of Bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Contact Info

Product

Resources

About