Isolation of Bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Kongrueng, Jetnapang; Mitraparp-Arthorn, Pimonsri; Bangpanwimon, Khotchawan; Robins, William; Vuddhakul, Varaporn; Mekalanos, John J.

doi:10.3354/dao03120

Cited by 29 publications

(14 citation statements)

References 38 publications

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“…vannamei ( Zhang et al, 2009 ; Suantika et al, 2013 ; Sangnoi et al, 2016 ). However, other unobserved microorganisms, such as bacteriophage, Bdellovibrio, Saccharomyces, Streptococcus, Streptomyces , and protists might also suppress Vibrio ( Munro et al, 2003 ; Alagappan et al, 2010 ; Chavez-Dozal et al, 2013 ; Lakshmi et al, 2013 ; Kongrueng et al, 2017 ). Considering final shrimp harvests, the intensive system is the more economically promising system.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Abundance and Community Composition in Pond Water From Shrimp Aquaculture Systems With Different Stocking Densities

et al. 2018

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In shrimp aquaculture, farming systems are carefully managed to avoid rearing failure due to stress, disease, or mass mortality, and to achieve optimum shrimp production. However, little is known about how shrimp farming systems affect biogeochemical parameters and bacterial communities in rearing water, whether high stocking densities (intensive system) will increase the abundance of pathogenic bacteria. In this study, we characterized bacterial communities in shrimp ponds with different population densities. Water quality, such as physical parameters, inorganic nutrient concentrations, and cultivable heterotrophic bacterial abundances, including potential pathogenic Vibrio, were determined in moderate density/semi-intensive (40 post-larvae m-3) and high density/intensive shrimp ponds (90 post-larvae m-3), over the shrimp cultivation time. Free-living and particle-attached bacterial communities were characterized by amplicon sequencing of the 16S rRNA gene. Suspended particulate matter (SPM), salinity, chlorophyll a, pH, and dissolved oxygen differed significantly between semi-intensive and intensive systems. These variations contrasted with the equal abundance of cultivable heterotrophic bacteria and inorganic nutrient concentrations. Bacterial communities were dominated by Gammaproteobacteria, Alphaproteobacteria, Flavobacteriia, Bacilli, and Actinobacteria. Halomonas and Psychrobacter were the most dominant genera in the particle-attached fractions, while Salegentibacter, Sulfitobacter, and Halomonas were found in the free-living fractions of both systems. Redundancy analysis indicated that among the observed environmental parameters, salinity was best suited to explain patterns in the composition of both free-living and particle-attached bacterial communities (R2: 15.32 and 12.81%, respectively), although a large fraction remained unexplained. Based on 16S rRNA gene sequences, aggregated particles from intensive ponds loaded a higher proportion of Vibrio than particles from semi-intensive ponds. In individual ponds, sequence proportions of Vibrio and Halomonas displayed an inverse relationship that coincided with changes in pH. Our observations suggest that high pH-values may suppress Vibrio populations and eventually pathogenic Vibrio. Our study showed that high-density shrimp ponds had a higher prevalence of Vibrio, increased amounts of SPM, and higher phytoplankton abundances. To avoid rearing failure, these parameters have to be managed carefully, for example by providing adequate feed, maintaining pH level, and removing organic matter deposits regularly.

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Section: Discussionmentioning

confidence: 99%

Bacterial Abundance and Community Composition in Pond Water From Shrimp Aquaculture Systems With Different Stocking Densities

et al. 2018

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“…Each 5 isolates of V. parahaemolyticus belonging to the tdh − trh 1 + , tdh − trh 2 + , tdh + trh 1 + and tdh + trh 2 + isolates was investigated for biofilm formation as previously described [ 20 ]. Biofilm formation was quantified by measuring the optical density (OD) at 570 nm using a LUMIstar Omega spectrophotometer (BMG LABTECH, Germany).…”

Section: Methodsmentioning

confidence: 99%

Characterization and CRISPR-based genotyping of clinical trh-positive Vibrio parahaemolyticus

et al. 2018

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BackgroundVibrio parahaemolyticus is a causative agent of gastroenteritis. Most of the clinical isolates carry either tdh and/or trh genes which are considered as the major virulence genes of this pathogen. In this study, the clinical isolates of V. parahaemolyticus carrying trh gene (n = 73) obtained from 1886 to 2012 from various countries were investigated for the urease production, haemolytic activity, and biofilm formation. In addition, the potential of clustered regularly interspaced short palindromic repeats (CRISPR)-based genotyping among these isolates was investigated.ResultsIn this study, no significant differences were observed in the urease production between tdh+ trh1+ and tdh+ trh2+ isolates (p = 0.063) and between the tdh− trh1+ and tdh− trh2+ isolates (p = 0.788). The isolates carrying only the trh gene showed variation in their haemolytic activity. The ratio of urease production and haemolytic activity between the trh1+ and trh2+ isolates and biofilm formation of trh+ V. parahaemolyticus isolates were not significantly different. Sixteen of thirty-four tested isolates (47.0%) of trh+ V. parahaemolyticus were positive for CRISPR detection. The discriminatory power index (DI) of CRISPR-virulence typing was higher than the DI obtained by CRISPR typing alone.ConclusionThe tdh and trh genes were not involved in urease production in the trh+ V. parahaemolyticus, and variation of haemolytic activity detected in V. parahaemolyticus carrying only the trh gene might be correlated to the sequence variation within trh1 and trh2 genes. Additionally, biofilm production of V. parahaemolyticus was not associated with harboring of virulence genes. For genotyping, CRISPR sequences combined with virulence genes can be used as genetic markers to differentiate trh+ V. parahaemolyticus strains.Electronic supplementary materialThe online version of this article (10.1186/s13099-018-0275-4) contains supplementary material, which is available to authorized users.

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“…Najnine et al (2020) extensively discuss the use of BALOs in aquaculture, pointing to the strong links between shrimp health and BALO abundance in shrimp guts, suggested by increases and decreases in BALOs versus Vibrionaceae respectively (Yang et al, 2016;Xiong et al, 2017;Najnine et al, 2020). Direct supplementation of BALOs to the water significantly improves the survival of white shrimp larvae (Litopenaeus vannamei) as shown by Kongrueng et al (2017): Halobacteriovorax spp. decrease post-larval mortality in the white shrimp (Litopenaeus vannamei) after treatment with acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus, also reducing biofilms formed by the pathogen (Kongrueng et al, 2017).…”

Section: Balos In Agriculture and In Aquaculturementioning

confidence: 99%

Interactions between Bdellovibrio and like organisms and bacteria in biofilms: beyond predator–prey dynamics

Mookherjee

Jurkevitch

2021

Environmental Microbiology

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Bdellovibrio and like organisms (BALOs) prey on Gram-negative bacteria in the planktonic phase as well as in biofilms, with the ability to reduce prey populations by orders of magnitude. During the last few years, evidence has mounted for a significant ecological role for BALOs, with important implications for our understanding of microbial community dynamics as well as for applications against pathogens, including drug-resistant pathogens, in medicine, agriculture and aquaculture, and in industrial settings for various uses. However, our understanding of biofilm predation by BALOs is still very fragmentary, including gaps in their effect on biofilm structure, on prey resistance, and on evolutionary outcomes of both predators and prey. Furthermore, their impact on biofilms has been shown to reach beyond predation, as they are reported to reduce biofilm structures of non-prey cells (including Grampositive bacteria). Here, we review the available literature on BALOs in biofilms, extending known aspects to potential mechanisms employed by the predators to grow in biofilms. Within that context, we discuss the potential ecological significance and potential future utilization of the predatory and enzymatic possibilities offered by BALOs in medical, agricultural and environmental applications.

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Isolation of Bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Cited by 29 publications

References 38 publications

Bacterial Abundance and Community Composition in Pond Water From Shrimp Aquaculture Systems With Different Stocking Densities

Bacterial Abundance and Community Composition in Pond Water From Shrimp Aquaculture Systems With Different Stocking Densities

Characterization and CRISPR-based genotyping of clinical trh-positive Vibrio parahaemolyticus

Interactions between Bdellovibrio and like organisms and bacteria in biofilms: beyond predator–prey dynamics

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