2017
DOI: 10.1371/journal.pntd.0005657
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LAMP-2 absence interferes with plasma membrane repair and decreases T. cruzi host cell invasion

Abstract: Trypanosoma cruzi enters host cells by subverting the mechanism of cell membrane repair. In this process, the parasite induces small injuries in the host cell membrane leading to calcium entry and lysosomal exocytosis, which are followed by compensatory endocytosis events that drive parasites into host cells. We have previously shown that absence of both LAMP-1 and 2, major components of lysosomal membranes, decreases invasion of T. cruzi into host cells, but the mechanism by which they interfere with parasite… Show more

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Cited by 16 publications
(18 citation statements)
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“…6H and 6I) when compared to wild type cells (Fig. 6G), similar to what was observed for T. cruzi infection with the same cell lines [25]. Additionally, our results indicate that Leishmania promastigotes are able to trigger calcium signaling in host cells from intracellular stores (Fig.…”
Section: Discussionsupporting
confidence: 88%
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“…6H and 6I) when compared to wild type cells (Fig. 6G), similar to what was observed for T. cruzi infection with the same cell lines [25]. Additionally, our results indicate that Leishmania promastigotes are able to trigger calcium signaling in host cells from intracellular stores (Fig.…”
Section: Discussionsupporting
confidence: 88%
“…To better evaluate the role of lysosomes in cell infection and specifically address whether PM repair is important for cell invasion, we performed the infection of LAMP-2 knockout and LAMP-1/2 double knockout MEFs with LLa -RFP. These cells are known to be deficient in PM repair due to the accumulation of cholesterol and caveolin in lysosomes and, for this reason, are less susceptible to the invasion of T. cruzi [25]. The results (Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…To better evaluate the role of lysosomes in cell infection and specifically address whether PM repair is important for cell invasion, we performed the LLa-RFP infection in LAMP2-knockout and LAMP1/2 double-knockout MEFs. These cells are known to be deficient in PM repair due to the accumulation of cholesterol and caveolin in lysosomes and, for this reason, are less susceptible to the invasion of T. cruzi (Couto et al, 2017). The results ( Fig.…”
Section: Amazonensis Persists and Replicates Within Lampcontainingmentioning
confidence: 92%
“…This decrease in entry is not accompanied by inhibition of lysosome exocytosis to the repair region as well as phenotypic modification of the host cell or generated PV. It is believed that the decrease in parasite entry is due to problems in caveolin-mediated endocytosis and in calcium efflux [67]. Considering that there are two distinct pools of lysosomes (cortical and internal) in mammalian cells, Hissa and Andrade used cardiomyocytes and observed, through the use of specific inhibitors, that trypomastigotes enter into this cell type mainly recruiting lysosomes from the more internal cell layer (perinuclear) [52].…”
Section: Mechanisms Of Internalization Of Trypanosoma Cruzi Trypomastmentioning
confidence: 99%