Laminin Immobilized on Titanium Oxide Films for Enhanced Human Umbilical Vein Endothelial Cell Adhesion and Growth

Ge, Sh.N.; Chen, Jun Ying; Leng, Yong Xiang; Huang, Nan

doi:10.4028/0-87849-436-7.305

Cited by 3 publications

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“…8) and heparin could not cover the collagen completely. So immunochemical staining of collagen on (col/hep) 15 surface shows slight fluorescence. Because interpenetration appears on the heparin layer, heparin layer as the outmost layer could simultaneously possess the property of heparin and collagen.…”

Section: Discussionmentioning

confidence: 99%

“…The result of collagen staining shows that collagen permeates in the heparin layer. Because the thickness of (col/hep) 15 was approximately 1370 6 100 nm, 36 the average thickness of each bilayer is 91.3 6 6.7 nm. Examining sensitive depth of XPS on nonmetal surface is approximately 6-10 nm.…”

Section: Discussionmentioning

confidence: 99%

“…To solve this problem, rapid re-endothelialization has been proposed and attempted by using a number of techniques in recent years. 14,15 This strategy is based on coating the biomaterial surface with an EC-compatible protein layer (such as ECM), which can stimulate the attachment and proliferation of the surrounding mature ECs and circulating EPCs on the surface of the blood contacting device and accelerate the formation of an active EC layer after device implantation. Thus, modifying the biomaterial surface to make it suitable for EC or EPC attachment and proliferation is the key for rapid re-endothelialization of blood contacting devices.…”

Section: Discussionmentioning

confidence: 99%

“…For the purpose of endothelialization, the development of substrates bearing cell-binding peptides or proteins that promote in vivo endothelialization is being pursued. 14,15 However, after implantation, exposed surface ligands intended to promote EC adhesion often bind platelets, thereby exacerbating surface thrombogenicity. [16][17][18] Based on knowledge of which vasoregulatory factors contribute to the exceptional biocompatibility of ECs, efforts have been made to use such factors to create more hemocompatible or cytocompatible surfaces.…”

Section: Introductionmentioning

confidence: 99%

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Collagen/heparin coating on titanium surface improves the biocompatibility of titanium applied as a blood‐contacting biomaterial

Chen

et al. 2010

J Biomedical Materials Res

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Thrombosis and restenosis are the main causes leading to failure of cardiovascular and other blood-contacting biomedical devices. It is recognized that rapid re-endothelialization is a promising method for preventing these complications. This article deals with improving the endothelial progenitor cell (EPC) compatibility and hemocompatibility of titanium by coating an extracellular matrix-like film with heparin(hep) and collagen(col) by a layer-by-layer (LBL) self-assembly technique. In the work described here, LBL-produced col/hep coating growth is initialized by deposition of a layer of poly-L-lysine on a titanium surface, which is negatively charged after treatment with NaOH, followed by formation of a multilayer film formed by alternating deposition of negatively charged heparin and positively charged collagen using electrostatic interaction. The X-ray photoelectron spectroscopy results and fluorescence staining of collagen show that collagen is predominant on the surface and that collagen interpenetrates the heparin layer. In vitro EPC attachment and proliferation increase greatly on the col/hep coating. Immunofluorescent staining of cytoskeleton actin reveals that cells on the col/hep coating form a compact confluent cell layer after culture for 3 days. After culture for 5 days, cell viability on the col/hep increases persistently and on titanium the cell viability begins to decrease, showing that the coating possesses the ability to maintain cell viability. Platelet adhesion under dynamic conditions in vitro implies that the hemocompatibility of the col/hep coating is superior to that of titanium. The col/hep coating improves the biocompatibility of titanium and has good potential for application in blood-contacting biomaterials.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Collagen/heparin coating on titanium surface improves the biocompatibility of titanium applied as a blood‐contacting biomaterial

Chen

et al. 2010

J Biomedical Materials Res

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“…Базальная мембрана состоит из сети мо-лекул, включающих коллаген IV типа, ламинин, гепарансульфат и протеогликан, в то время как типичными компонентами интерстициального матрикса являются фибриллярные коллагены и гликопротеины (фибронектин и витронектин). Многочисленные исследования были проведены с иммобилизацией адгезивных молекул, имити-рующих ВМ, например, фибронектин, коллаген и ламинин [32][33][34][35]. Однако эти молекулы усили-вали адгезию не только эндотелиальных клеток, но и тромбоцитов, а также стимулировали проли-ферацию гладкомышечных клеток [36,37].…”

Section: имитация структур внеклеточного матрикса на поверхности метаunclassified

Surface Modification of Bare-Metal Stents for Preventing Restenosis (Part 2)

Лотков¹,

Матвеева²,

Антонова³

et al. 2017

Kompleks. probl. serdečno-sosud. zabol.

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Резюме. Вторая часть обзора посвящена вариантам модификации металлических стентов для ускоре-ния эндотелизации in situ. Представлены разработки. направленные на захват эндотелиальных прогени-торных клеток из кровотока с помощью специфических антител. Описаны возможности улучшения адгезии и интеграции эндотелиальных клеток на поверхности за счет формирования сайтов клеточного распозна-вания и имитация структур внеклеточного матрикса. Проанализированы различные способы физической и химической модификации, способствующие созданию условий для скорейшего формирования функцио-нально состоятельного эндотелиального слоя на искусственных поверхностях. Определен круг проблем и ограничений в использовании каждого из методов.Ключевые слова: металлические стенты, рестеноз, модификация поверхности, эндотелизация. Here we review the modifications of the metal stents to enhance in situ endothelialization, particularly antibody-mediated adhesion of endothelial progenitor cells. We focus on the strategies employing cell adhesion molecules and extracellular matrix proteins. We also encompass other physical and chemical approaches for surface biofunctionalization with their advantages and shortcomings. SURFACE MODIFICATION OF BARE-METAL STENTS FOR PREVENTING RESTENOSISKeywords: bare-metal stents, restenosis, surface modification, endothelialization НИИ КПССЗ имеет большой опыт в разработке изделий для сердечно-сосудистой хирургии, в частности биопротезов кла-панов сердца и сосудов. С 2010 года под руководством академика РАН л. С. Барбараша начаты исследования по созданию био-резорбируемых сосудистых протезов малого диаметра. Основная цель -создание полимерных конструкций с бионаправленным действием, способных заменить отдельные структуры живого организма, в частности сердечно-сосудистой системы. Научная но-визна поставленной задачи заключается в использовании нового подхода создания органа непосредственно в организме пациента за счет биофункциональности и биорезорбируемости полимерных конструкций. доказана долгосрочная проходимость полимерных сосудистых графтов на основе поликапролактона и композиции полигидроксибутирата/валерата и поликапролактона. В экспери-ментах in vitro доказано, что ростовые факторы, инкорпорируемые в состав биодеградируемых графтов, сохраняют свою био-логическую активность. В долгосрочных экспериментах in vivo доказано, что сосудистый эндотелиальный фактор роста ускоряет эндотелизацию и улучшает проходимость биодеградируемых полимерных сосудистых графтов.Ключевые слова: биодеградируемые полимеры, сосудистый графт, ростовые факторы. DEVELOPMENT OF TISSUE ENGINEERED SMALL DIAMETER VASCULAR GRAFT FOR THE CARDIOVASCULAR SURGERY NEEDS L. V. ANTONOVA, YU. A. KUDRYAVTSEVA Federal State Budgetary Scientific Institution Research Institute for Complex Issues of Cardiovascular Diseases. Kemerovo, RussiaRICICd has large experience in the development of products for cardiovascular surgery, in particular, the bioprosthesis heart valves and blood vessels. Since 2010, under the leadership of the academician of RAS L. S. Bar...

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The effect of surface modification of poly‐lactide‐co‐glycolide/carbon nanotube nanofibrous scaffolds by laminin protein on nerve tissue engineering

Nazeri

Karimi

Ghanbari

2020

J Biomedical Materials Res

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The presence of biological cues to promote the attachment, proliferation, and differentiation of neuronal cells is important in the process of nerve regeneration. In this study, laminin as a neurite promoting protein, has been used to modify polylactide-co-glycolide/carbon nanotube (PLGA/CNT) electrospun nanofibrous scaffolds by means of either mussel-inspired poly(dopamine) (PD) coating or via direct physical adsorption as a simple route for the functionalization of biomaterials. The lamininmodified scaffolds were characterized by a combination of field emission scanning electron microscopy (SEM), X-ray photoelectron spectroscopy, and contact angle measurements. Subsequently, various properties of scaffolds such as degradation time, amount of attached laminin and the rate of CNT release were investigated. The synergistic effect of topographical and biological cues for PC12 cell attachment, proliferation, and differentiation were then studied by SEM and confocal microscopy. The results of degradation study showed that laminin-modified scaffolds were biodegradable with good structural integrity that persisted about 4 weeks. The amount of laminin attached to the PLGA/CNT and PLGA/CNT-PD scaffolds was 3.12 ± 0.6 and 3.04 ± 071 μg per mg of the scaffold, respectively. Although laminin-modified scaffolds could improve cell proliferation identically, neurite extensions on the PLGA/ CNT scaffold modified via PD coating (PLGA/CNT-PD-lam scaffold) were significantly longer than those observed on PLGA/CNT scaffold modified via physical adsorption (PLGA/CNT-lam scaffold) and unmodified scaffolds. Together, these results indicated that surface modification via PD coating could be a promising strategy to fabricate biomimetic scaffolds capable of sustaining longer neuronal growth for nerve tissue engineering.

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Laminin Immobilized on Titanium Oxide Films for Enhanced Human Umbilical Vein Endothelial Cell Adhesion and Growth

Cited by 3 publications

References 0 publications

Collagen/heparin coating on titanium surface improves the biocompatibility of titanium applied as a blood‐contacting biomaterial

Collagen/heparin coating on titanium surface improves the biocompatibility of titanium applied as a blood‐contacting biomaterial

Surface Modification of Bare-Metal Stents for Preventing Restenosis (Part 2)

The effect of surface modification of poly‐lactide‐co‐glycolide/carbon nanotube nanofibrous scaffolds by laminin protein on nerve tissue engineering

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