Collagen/heparin coating on titanium surface improves the biocompatibility of titanium applied as a blood‐contacting biomaterial

Abstract

Thrombosis and restenosis are the main causes leading to failure of cardiovascular and other blood-contacting biomedical devices. It is recognized that rapid re-endothelialization is a promising method for preventing these complications. This article deals with improving the endothelial progenitor cell (EPC) compatibility and hemocompatibility of titanium by coating an extracellular matrix-like film with heparin(hep) and collagen(col) by a layer-by-layer (LBL) self-assembly technique. In the work described here, LBL-produced col/hep coating growth is initialized by deposition of a layer of poly-L-lysine on a titanium surface, which is negatively charged after treatment with NaOH, followed by formation of a multilayer film formed by alternating deposition of negatively charged heparin and positively charged collagen using electrostatic interaction. The X-ray photoelectron spectroscopy results and fluorescence staining of collagen show that collagen is predominant on the surface and that collagen interpenetrates the heparin layer. In vitro EPC attachment and proliferation increase greatly on the col/hep coating. Immunofluorescent staining of cytoskeleton actin reveals that cells on the col/hep coating form a compact confluent cell layer after culture for 3 days. After culture for 5 days, cell viability on the col/hep increases persistently and on titanium the cell viability begins to decrease, showing that the coating possesses the ability to maintain cell viability. Platelet adhesion under dynamic conditions in vitro implies that the hemocompatibility of the col/hep coating is superior to that of titanium. The col/hep coating improves the biocompatibility of titanium and has good potential for application in blood-contacting biomaterials.

Simultaneous characterization of physical, chemical, and thermal properties of polymeric multilayers using infrared spectroscopic ellipsometry

“…PEI/PSS LbL films have been previously used as sorbents for CO 2 capture [21], adsorption of prussian blue colloids [32], and corrosion control [33]. The natural system of multilayers COL/HEP have been previously used to help promote the blood-compatibility of titanium implants [34][35][36][37], vascularization studies [38] and intravascular endothelialization with the functionalization of antibodies over the multilayers [39][40]. IRVASE was used to study the effect of changes in temperature (25, 37, 50, 75, 100, 125 and 150 °C) and pH (2.7, 4, 5 and 6) of the washing solution used to prepare the multilayers with respect to changes in thickness, roughness and chemical composition.…”

Biomimetic modification of metallic cardiovascular biomaterials: from function mimicking to endothelialization in vivo

“…In our study, an ECM-like hydrogel coating of collagen and SCS was also obtained, which is beneficial for improved biocompatibility. A collagen and heparin LBL supramolecular film was also prepared by Chen et al [43]. It shows that the modified surface reduces platelet adhesion under dynamic conditions and possesses the ability to maintain cell viability.…”

In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments

“…As reported in an our previous paper [13], collagen coating can be also promoted by low temperature nitriding treatment of AISI 316L austenitic stainless steel, and this collagen protection may be favourable for endothelial cell proliferation and for the control of metalloproteinase (MMP)-2 release. Collagen, the major component of extracellular matrix, shows high conformational stability, little inflammatory reaction and good cytocompatibility [14]. This protein is also involved in many biological functions, as cell attachment and tissue regeneration, and, for these reasons, it has been used to modify the surface of medical devices in order to induce cell-material interactions.…”