Laminin and collagen IV subunit distribution in normal and neoplastic tissues of colorectum and breast

Hewitt, Robert E.; Powe, Desmond G.; Morrell, Kayla; Balley, E; Leach, I. H.; Ellis, Ian O.; Turner, David R.

doi:10.1038/bjc.1997.37

Cited by 64 publications

(64 citation statements)

References 20 publications

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“…Yet, its precise involvement in tumorigenicity and metastasis is not clearly elucidated as data from the literature are somewhat controversial. On 1 hand, colorectal cancer malignancy has been correlated with decreased or interrupted immunostaining of all basement membrane components at the tumor cell/fibroblasts interface, 12,14,[33][34][35] due either to deficiency in their production or to their degradation by specific proteases. On the other hand, when reconstituted basement membrane (Matrigel) or extracted laminin-1 has been coinjected in animals with various human or murine tumor cell lines, both the incidence and the growth of tumors were increased; 17,36 -39 in these types of experiments, the possibility that growth factors were copurified with the basement membrane components cannot be discarded.…”

Section: Discussionmentioning

confidence: 99%

“…In colorectal cancer, perturbations in the production, deposition and degradation of matrix molecules, among which are laminins, have been observed. [12][13][14][15][16] The role of laminin-1 in tumor growth and metastasis has been evaluated in several cell systems. In particular, it was shown that coinjection of purified laminin-1 and of lung or colon cancer cells increases the growth of the tumors developed as xenografts in nude mice; such an effect was not observed when collagen IV, another basement membrane molecule, was used.…”

Section: Abstract: Laminins; Basement Membrane; Colonic Cancer Cellsmentioning

confidence: 99%

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Overexpression of laminin ?1 chain in colonic cancer cells induces an increase in tumor growth

et al. 2001

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Laminins represent a growing family of glycoproteins constituting the basement membrane. They are known to direct many biological processes. With respect to carcinogenesis, laminins play an important role in cell adhesion, mitogenesis, differentiation and even metastasis. To further study the biological significance of laminin-1 (composed of ␣1, ␤1 and ␥1 chains) in intestinal cell differentiation or tumorigenesis, an ␣1-laminin expression vector was introduced into the HT29 colonic cancer cells, in which laminin ␣1 chain is not expressed. Upon transfection of the ␣1 chain, the ␣1␤1␥1 trimer was found secreted in the media along with free Key words: laminins; basement membrane; colonic cancer cells; tumor growth; differentiationIt is well established that in multicellular organisms, extracellular matrix molecules control various physiologic activities such as cell growth, migration, apoptosis and differentiation. The extracellular network includes the interstitial matrix and basement membranes. The basement membranes that separate the connective tissue from epithelia, muscle fibers, blood vessels and nerves are formed by a complex set of collagens and of noncollagenous glycoproteins such as laminins, nidogen and of proteoglycans, which have a unique composition in each organ. Laminins are multifunctional molecules forming a family of proteins that display organ, site and developmental specificity. 1,2 The first characterized laminin isoform, laminin-1 (composed of ␣1, ␤1 and ␥1 chains), is a crosslike-shaped molecule assembled into a triplestranded coiled-coil structure designated as the long arm. 3 As for the other extracellular matrix molecules, laminin-1 is made up of specific structural domains that allow self-assembly, permit interaction with other basement membrane molecules and encode information for the cells via specific transmembrane receptors, mostly of the integrin family. 4 Recent studies performed on various organs during development show that laminin-1 has a rather limited expression. 5 Despite such restricted expression, crucial roles for laminin-1 in organ development and in cell differentiation have been demonstrated by cell-coating experiments, blocking antibody and antisense strategies. Several major functions have been assigned to the constitutive ␣1 chain of laminin-1, among which are the polarization of developing kidney tubules, 6,7 lung alveolar formation, 8 development of submandibular glands 9 and polarization and functional differentiation of mammary and intestinal cells. 10,11 Conversely, alterations in cell-matrix and cell-cell interactions have been shown in a number of pathologic situations, such as inflammatory processes, wound healing and malignant transformation. In colorectal cancer, perturbations in the production, deposition and degradation of matrix molecules, among which are laminins, have been observed. [12][13][14][15][16] The role of laminin-1 in tumor growth and metastasis has been evaluated in several cell systems. In particular, it was shown that coinjection of puri...

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Section: Discussionmentioning

confidence: 99%

Section: Abstract: Laminins; Basement Membrane; Colonic Cancer Cellsmentioning

confidence: 99%

Overexpression of laminin ?1 chain in colonic cancer cells induces an increase in tumor growth

et al. 2001

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“…Another family of ECM molecules that requires further study in this context is that of the collagen IV isoforms. Their expression has been shown to be regulated independently of laminins in both normal and neoplastic gut (Perreault et al 1998;Hewitt et al 1997).…”

Section: Donaldmentioning

confidence: 99%

Unaltered Distribution of Laminins, Fibronectin, and Tenascin in Celiac Intestinal Mucosa

Korhonen

Ormio²,

Burgeson³

et al. 2000

J Histochem Cytochem.

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Extensive remodeling of the extracellular matrix (ECM) occurs in inflammatory tissues. The celiac lesion in the small intestine is characterized by inflammation accompanied by profound morphological alterations. We used immunohistochemistry to determine the distribution of laminin, fibronectin, and tenascin isoforms in small intestinal biopsies of untreated patients with celiac disease. In normal mucosa, the distribution of laminin isoforms defines three epithelial basement membrane (BM) zones. We found that the organization of these zones was maintained in the celiac mucosa. Thus, components of laminin-5 (α3 and β3) were found in the surface epithelial BM, laminin α2 chain was found selectively at crypt bottoms, and laminin α5 chain was the sole α-type chain in middle crypt BMs. Likewise, the distribution of fibronectin and tenascin resembled that of the normal gut. The organization of pericryptal fibroblasts and lamina propria smooth muscle strands, as defined by immunostaining for α-smooth muscle actin, also remained unchanged in the celiac mucosa. Unexpectedly, major ECM changes were not detected in the celiac lesion.

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“…Magnifications: A ϫ100; B ϫ250; C ϫ200. Vermeulen et al 1995;Hewitt et al 1997). Consequently, these vessels do not fit well into the usual classification of arterioles, capillaries, or venules.…”

Section: Discussionmentioning

confidence: 99%

Angioarchitecture of Primary Oral Malignant Melanomas

Lee

Nagai

Siar

et al. 2002

J Histochem Cytochem.

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S U M M A R YAngiogenesis is an essential process in the progression of malignant tumors. However, little is known of the angioarchitecture in primary oral malignant melanoma. We sought to determine this by the use of periodic acid-Schiff (PAS) stain, endothelial markers (CD34, CD105) and laminin, and by transmission electron microscopy in two cases. The results demonstrated that endothelium-lined vessels dominated the tumor microvasculature and these stained positively for PAS, laminin, and endothelial markers. Mosaic and tumorlined vessels were infrequently encountered. Most PAS-positive patterned networks and loops ultrastructurally represented intratumor microhemorrhages that probably arose secondary to tumor vessel leakiness. Vascular channels of the vasculogenic mimicry type were rare. They stained for laminin but not for endothelial markers.

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Laminin and collagen IV subunit distribution in normal and neoplastic tissues of colorectum and breast

Cited by 64 publications

References 20 publications

Overexpression of laminin ?1 chain in colonic cancer cells induces an increase in tumor growth

Overexpression of laminin ?1 chain in colonic cancer cells induces an increase in tumor growth

Unaltered Distribution of Laminins, Fibronectin, and Tenascin in Celiac Intestinal Mucosa

Angioarchitecture of Primary Oral Malignant Melanomas

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