Laminin‐5 gamma 2 chain is colocalized with gelatinase‐A (MMP‐2) and collagenase‐3 (MMP‐13) in odontogenic keratocysts

Abstract

Our results indicate that the colocalization of Ln-5 with MMP-2 or MMP-13, but not with MMP-8, in BM zone of KCs, may be related to special characteristics of KC.

Immunohistochemical expression of MMPs 1, 7, and 26 in syndrome and nonsyndrome odontogenic keratocysts

“…It also favors epithelial migration and OKC growth. 33 Based on the reports revealing an important role for MMP-2 in OKC biology, 33,34 the immunohistochemical expression of MMP-7 in SOKCs and NSOKCs observed in our study shows a possible additional importance of this protease. The presence of matrilysin 1 in OKCs may contribute to the activation of proenzyme forms of MMP-2, 26,27 which could degrade the surrounding extracellular matrix and favor epithelial migration and OKC growth.…”

“…Many of these have been concerned with identifying MMPs-1, Ϫ2, Ϫ8, and Ϫ13 [32][33][34] in OKCs alone or with comparing them to other odontogenic cysts, such as radicular and dentigerous cysts. To date, this is the first study to assess comparatively the immunoreactivity to MMPs-1, Ϫ7, and Ϫ26 between SOKCs and NSOKCs.…”

“…26,27 MMPs-2 and Ϫ9, also known as gelatinases, are important proteases that degrade the components present both in basement membranes, such as type IV collagen and laminin, 15,36 and in the interstitial extracellular matrix, particularly the fragments derived from the proteolysis of type I fibrillar collagen, denominated gelatins. 18,36 MMP-2 has been observed by immunohistochemistry in OKC epithelial basement membranes, 33 by in situ hybridization in OKC connective tissue fibroblasts, 33 by western immunoblotting and gelatin zymography in cultured fibroblasts, 32 and in co-cultured OKC epithelial cells and fibroblasts. 34 In light of these findings, MMP-2 has been implied in the degradation of the extracellular matrix surrounding OKCs.…”

“…The presence of matrilysin 1 in OKCs may contribute to the activation of proenzyme forms of MMP-2, 26,27 which could degrade the surrounding extracellular matrix and favor epithelial migration and OKC growth. 33,34 The possibility of interactions between WNT/␤-catenin and PTCH/SHH intracellular signaling pathways might be one of the causes of the higher immunohistochemical expression of MMPs-7 and Ϫ26 in SOKCs. Recent studies in neoplasms associated with the Gorlin syndrome, such as medulloblastomas 37 and craniopharyngiomas, 38 showed that PTCH gene mutations were associated with an overexpression of several components of the WNT intracellular signaling pathway, mainly ␤-catenin.…”

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

“…53 [56][57][58] Under normal conditions, degradation and synthesis of ECM components is in balance, so that these MMPs are expressed at very low levels, if at all. Whenever active tissue remodelling is required, their production and activation increase dramatically.…”