Laminated three-dimensional biodegradable foams for use in tissue engineering

Mikos, Antonios G.; Sarakinos, Georgios; Leite, Susan M.; Vacanti, Joseph P.; Langer, Robert

doi:10.1016/0142-9612(93)90049-8

Cited by 618 publications

(241 citation statements)

References 11 publications

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“…Publicadas na literatura científica (concomitantemente às requisições de patentes nos EUA), as técnicas de preparo de suportes porosos foram descritas em culturas de células específicas, e ainda representam um importante enfoque de desenvolvimentos [41] . Entre as técnicas podemos destacar: fiber bonding [42] , evaporação de solvente com adição e lixiviação de sal (solvent casting -particulate leaching) [43] , inversão de fases [44] , injeção de gás [45] , fused deposition modeling (FDM) [46] , freeze-dried [47] e outras.…”

Section: Técnicas De Preparo Dos Suportes Porososunclassified

Polímeros bioreabsorvíveis na engenharia de tecidos

Barbanti

Zavaglia

Duek³

2005

Polímeros

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[3] , sistemas para liberação controlada de drogas [4] , stents [5] e dispositivos ortopédicos [6] . Atualmente fazem parte do cotidiano dos centros cirúrgicos no mundo inteiro.Embora muitos dispositivos protéticos artificiais estejam disponíveis, poucos podem substituir completamente todas as complexas funções biológicas. Em situações clíni-cas mais severas somente o transplante do órgão retoma as atividades orgânicas. Assim, de uma forma idealizada, a melhor alternativa seria obter um novo órgão ou tecido, substituindo aquele que não desempenha normalmente suas funções. Nos dias de hoje, a idéia da reconstrução de órgãos e tecidos criados em laboratório é amplamente difundida e investigada no mundo todo [7,8] . Resumo: A Engenharia de Tecidos consiste em um conjunto de conhecimentos e técnicas para a reconstrução de novos órgãos e tecidos. Baseada em conhecimentos das áreas de ciência e engenharia de materiais, biológica e médica, a técnica envolve a expansão in vitro de células viáveis do paciente doador sobre suportes de polímeros bioreabsorvíveis. O suporte degrada enquanto um novo órgão ou tecido é formado. Os poli(α-hidróxi ácidos) representam a principal classe de polímeros sintéticos bioreabsorvíveis e biodegradáveis utilizados na engenharia de tecidos. No desenvolvimento e na seleção desses materiais, o tempo de degradação é fundamental para o sucesso do implante. Os estudos e os desafios atuais são normalmente direcionados ao entendimento das relações entre composição química, cristalinidade, morfologia do suporte, e o processamento desses materiais. Este artigo faz uma revisão dos trabalhos recentes sobre a utilização dos polímeros sintéticos bioreabsorvíveis como suportes na engenharia de tecidos. Engenharia de tecidos Palavras-chave: Engenharia de tecidos, polímeros bioreabsorvíveis, poli(α-hidróxi ácidos). Bioresorbable Polymers in Tissue EngineeringAbstract: Tissue Engineering is based on a group of techniques for the reconstruction of new organs and tissues. Based on knowledge of materials science and engineering, biology and medicine, the technique involves the in vitro expansion of viable cells obtained from the patient on the polymeric scaffolds. The scaffold degrades while a new organ or tissue is formed. The poly(α-hydroxy acids) are the principal biodegradable and bioresorbable polymers used in tissue engineering. In developing and selecting bioresorbable scaffolds, the degradation time is fundamental for successful biocompatibility and biofuncionality. Hence, degradation studies often address variables such as the chemical composition, crystallinity, morphology of the scaffold and the processing of these materials. This paper reviews recent work in bioresorbable polymers used as scaffolds in the tissue engineering. Keywords: Tissue engineering, bioresorbable polymers, poly(α-hydroxy acids). IntroduçãoQuando a estrutura biológica de um órgão ou tecido não pode ser reparada, a alternativa viável para o restabelecimento das funções normais do paciente é repô-la com um implante feito de um b...

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Section: Técnicas De Preparo Dos Suportes Porososunclassified

Polímeros bioreabsorvíveis na engenharia de tecidos

Barbanti

Zavaglia

Duek³

2005

Polímeros

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“…This method allows the control of the porosity and pore size by changing the amount and/or size of leachable particles even if it is more difficult to achieve good interconnectivity of pores. In the past, to overcome this deficiency, high porosity levels and interconnectivity were achieved by combining leaching with gas foaming [45], solvent casting [46,47], freeze drying [48,49] or immersion precipitation [50,51]. More recently, melt blending of two immiscible polymers has emerged as a new method of fabricating porous scaffolds for tissue engineering, particularly by creating co-continuous blend morphologies [52,53].…”

Section: Introductionmentioning

confidence: 99%

3D polylactide-based scaffolds for studying human hepatocarcinoma processesin vitro

Scaffaro

Rigogliuso

et al. 2012

Science and Technology of Advanced Materials

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We evaluated the combination of leaching techniques and melt blending of polymers and particles for the preparation of highly interconnected three-dimensional polymeric porous scaffolds for in vitro studies of human hepatocarcinoma processes. More specifically, sodium chloride and poly(ethylene glycol) (PEG) were used as water-soluble porogens to form porous and solvent-free poly(L,D-lactide) (PLA)-based scaffolds. Several characterization techniques, including porosimetry, image analysis and thermogravimetry, were combined to improve the reliability of measurements and mapping of the size, distribution and microarchitecture of pores. We also investigated the effect of processing, in PLA-based blends, on the simultaneous bulk/surface modifications and pore architectures in the scaffolds, and assessed the effects on human hepatocarcinoma viability and cell adhesion. The influence of PEG molecular weight on the scaffold morphology and cell viability and adhesion were also investigated. Morphological studies indicated that it was possible to obtain scaffolds with well-interconnected pores of assorted sizes. The analysis confirmed that SK-Hep1 cells adhered well to the polymeric support and emitted surface protrusions necessary to grow and differentiate three-dimensional systems. PEGs with higher molecular weight showed the best results in terms of cell adhesion and viability.

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“…Isolated cells cannot, however, form new tissues on their own. They require a specific environment, in which a supporting material acts as a template for cell growth [4][5][6][7] . Three-dimensional scaffolds can be used to mimic the natural counterparts: the extracellular matrix of the body.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable and macroporous polylactide implants for cell transplantation: 1. Preparation of macroporous polylactide supports by solid-liquid phase separation

Schugens

Maquet

Grandfils

et al. 1996

Polymer

251

149

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Freeze-drying of polylactide solutions in 1,4-dioxane has been studied as a way to produce microcellular foams. The thermally induced phase separation has been studied in relation to several processing and formulation parameters. The effects of polymer concentration, chain stereoregularity, polymer molecular weight and cooling rate have been investigated in connection with the porous morphology and the physico-mechanical characteristics of the final foams. As a rule, bundles of channels are formed with a diameter of ~100 µ.m. They have a preferential orientation that fits the cooling direction. A porous substructure (~10 µm) is observed in the internal walls of the tubular macropores. Variations in this general porous morphology-and particularly in the porosity, density, solvent residue, mechanical resistance and degree of regularity in the spatial organization of pores-have been observed when polymer concentration in 1,4-dioxane and polylactide stereoregularity are changed. As expected, cooling rate has a strong effect on the foam morphology, which is essentially controlled by the solvent crystallization. Pores are nothing but the fingerprints of 1,4-dioxane crystallites.

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Laminated three-dimensional biodegradable foams for use in tissue engineering

Cited by 618 publications

References 11 publications

Polímeros bioreabsorvíveis na engenharia de tecidos

Polímeros bioreabsorvíveis na engenharia de tecidos

3D polylactide-based scaffolds for studying human hepatocarcinoma processesin vitro

Biodegradable and macroporous polylactide implants for cell transplantation: 1. Preparation of macroporous polylactide supports by solid-liquid phase separation

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