Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress

Yao, Xuerui; Jiang, Hao; Liang, Shuang; Shen, Xiaopei; Gao, Qingshan; Xu, Yong; Kim, Nam‐Hyung

doi:10.1262/jrd.2018-031

Cited by 19 publications

(19 citation statements)

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“…Our results showed that LC treatment decreased the percentage of early apoptotic cells, which is in accordance with a previous finding that antioxidants reduce the level of early apoptosis in aging oocytes [48]. Caspase-3 is a member of the cysteine-aspartic protease (caspase) family [49]. Caspases are crucial mediators of programmed cell death (apoptosis).…”

Section: Discussionsupporting

confidence: 92%

L-carnitine prevents bovine oocyte aging and promotes subsequent embryonic development

Jiang

Yao

Zhao

et al. 2019

Journal of Reproduction and Development

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L-carnitine (LC) is well known for its antioxidant activity. In this study, we explored the potential mechanistic effects of LC supplementation on aged bovine oocytes in vitro. We showed that in-vitro maturation could enhance the subsequent developmental capacity of aging oocytes, when supplemented with LC. After in vitro fertilization, the blastocyst formation rate in the aged oocytes post-LC treatment significantly increased compared to that in untreated aged oocytes (29.23 ± 2.20% vs. 20.90 ± 3.05%). Furthermore, after LC treatment, the level of intracellular reactive oxygen species in aged oocytes significantly decreased, and glutathione levels significantly increased, compared to those in untreated aged oocytes. Mitochondrial membrane potential, the percentage of early apoptotic oocytes, and caspase-3 activity were significantly reduced in LC-treated aged oocytes compared to those in untreated aged oocytes. Furthermore, during in vitro aging, the mRNA levels of the anti-apoptotic genes, Bcl-xl and survivin in LC-treated aged oocytes were significantly higher than those in untreated aged oocytes. Overall, these results indicate that at least in in vitro conditions, LC can prevent the aging of bovine oocytes and improve the developmental capacity of bovine embryo.

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Section: Discussionsupporting

confidence: 92%

L-carnitine prevents bovine oocyte aging and promotes subsequent embryonic development

Jiang

Yao

Zhao

et al. 2019

Journal of Reproduction and Development

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“…Cathepsin B is closely associated with the process of apoptosis by activating caspases 48 . Similar to previous studies 44 , 49 , we also observed a significant rise in the activities of caspase-3 and cathepsin B during oocyte aging in vitro. In addition, Ax is known to be able to regulate the mitochondria-mediated apoptosis probably caused by signaling pathways such as NF-κB and Wnt/β-catenin, involving a downregulation of pro-apoptotic proteins (eg.…”

Section: Discussionsupporting

confidence: 91%

“…GSH as an endogenous antioxidant not only maintain the intracellular redox balance, but also is an indicator of cytoplasmic maturation to reflect the oocyte fertilization and subsequent development 41 . There was no reduction of GSH content for aged oocytes in this study, similar to previous reports 42 , 43 ; however, several studies have showed the aged oocytes with lower level of GSH 44 , 45 . Moreover, the aged oocytes treated with Ax exhibited an increase in intracellular GSH content, which is similar to the result of antioxidant hesperetin treatment 42 .…”

Section: Discussionsupporting

confidence: 91%

Inhibitory effects of astaxanthin on postovulatory porcine oocyte aging in vitro

Jia

Xiang

Shao

et al. 2020

Sci Rep

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Mammalian oocytes represent impaired quality after undergoing a process of postovulatory aging, which can be alleviated through various effective ways such as reagent treatment. Accumulating evidences have revealed the beneficial effects of astaxanthin (Ax) as a potential antioxidant on reproductive biology. Here, porcine matured oocytes were used as a model to explore whether Ax supplement can protect against oocyte aging in vitro and the underlying mechanism, and therefore they were cultured with or without 2.5 μM Ax for an additional 24 h. Aged oocytes treated with Ax showed improved yield and quality of blastocysts as well as recovered expression of maternal genes. Importantly, oxidative stress in aged oocytes was relieved through Ax treatment, based on reduced reactive oxygen species and enhanced glutathione and antioxidant gene expression. Moreover, inhibition in apoptosis and autophagy of aged oocyte by Ax was confirmed through decreased caspase-3, cathepsin B and autophagic activities. Ax could also maintain spindle organization and actin expression, and rescue functional status of organelles including mitochondria, endoplasmic reticulum, Golgi apparatus and lysosomes according to restored fluorescence intensity. In conclusion, Ax might provide an alternative for ameliorating the oocyte quality following aging in vitro, through the mechanisms mediated by its antioxidant properties.

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“…Mitochondria not only control energy metabolism in the body but also regulate apoptosis or necrosis (Wang et al, 2017). During the in vitro culture of oocytes, oxidative stress directly affects mitochondrial function (Yao et al, 2018). During early embryo culture, the body needs to provide strict energy in the form of adenosine triphosphate (Spinaci et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Supplementation of kaempferol to in vitro maturation medium regulates oxidative stress and enhances subsequent embryonic development in vitro

Zhao

et al. 2019

Zygote

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SummaryKaempferol (KAE) is one of the most common dietary flavonols possessing biological activities such as anticancer, anti-inflammatory and antioxidant effects. Although previous studies have reported the biological activity of KAE on a variety of cells, it is not clear whether KAE plays a similar role in oocyte and embryo in vitro culture systems. This study investigated the effect of KAE addition to in vitro maturation on the antioxidant capacity of embryos in porcine oocytes after parthenogenetic activation. The effects of kaempferol on oocyte quality in porcine oocytes were studied based on the expression of related genes, reactive oxygen species, glutathione and mitochondrial membrane potential as criteria. The rate of blastocyst formation was significantly higher in oocytes treated with 0.1 µm KAE than in control oocytes. The mRNA level of the apoptosis-related gene Caspase-3 was significantly lower in the blastocysts derived from KAE-treated oocytes than in the control group and the mRNA expression of the embryo development-related genes COX2 and SOX2 was significantly increased in the KAE-treated group compared with that in the control group. Furthermore, the level of intracellular reactive oxygen species was significantly decreased and that of glutathione was significantly increased after KAE treatment. Mitochondrial membrane potential (ΔΨm) was increased and the activity of Caspase-3 was significantly decreased in the KAE-treated group compared with that in the control group. Taken together, these results suggested that KAE is beneficial for the improvement of embryo development by inhibiting oxidative stress in porcine oocytes.

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Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress

Cited by 19 publications

References 50 publications

L-carnitine prevents bovine oocyte aging and promotes subsequent embryonic development

L-carnitine prevents bovine oocyte aging and promotes subsequent embryonic development

Inhibitory effects of astaxanthin on postovulatory porcine oocyte aging in vitro

Supplementation of kaempferol to in vitro maturation medium regulates oxidative stress and enhances subsequent embryonic development in vitro

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