L-carnitine prevents bovine oocyte aging and promotes subsequent embryonic development

Jiang, Wen-Jie; Yao, Xuerui; Zhao, Yuhan; Gao, Qingshan; Jin, Qingguo; Li, Yinghua; Yan, Ang-Guo; Xu, Yong‐Nan

doi:10.1262/jrd.2019-046

Cited by 25 publications

(30 citation statements)

References 58 publications

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“…GSH as an endogenous antioxidant not only maintain the intracellular redox balance, but also is an indicator of cytoplasmic maturation to reflect the oocyte fertilization and subsequent development 41 . There was no reduction of GSH content for aged oocytes in this study, similar to previous reports 42 , 43 ; however, several studies have showed the aged oocytes with lower level of GSH 44 , 45 . Moreover, the aged oocytes treated with Ax exhibited an increase in intracellular GSH content, which is similar to the result of antioxidant hesperetin treatment 42 .…”

Section: Discussionsupporting

confidence: 91%

Inhibitory effects of astaxanthin on postovulatory porcine oocyte aging in vitro

Jia

Xiang

Shao

et al. 2020

Sci Rep

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Mammalian oocytes represent impaired quality after undergoing a process of postovulatory aging, which can be alleviated through various effective ways such as reagent treatment. Accumulating evidences have revealed the beneficial effects of astaxanthin (Ax) as a potential antioxidant on reproductive biology. Here, porcine matured oocytes were used as a model to explore whether Ax supplement can protect against oocyte aging in vitro and the underlying mechanism, and therefore they were cultured with or without 2.5 μM Ax for an additional 24 h. Aged oocytes treated with Ax showed improved yield and quality of blastocysts as well as recovered expression of maternal genes. Importantly, oxidative stress in aged oocytes was relieved through Ax treatment, based on reduced reactive oxygen species and enhanced glutathione and antioxidant gene expression. Moreover, inhibition in apoptosis and autophagy of aged oocyte by Ax was confirmed through decreased caspase-3, cathepsin B and autophagic activities. Ax could also maintain spindle organization and actin expression, and rescue functional status of organelles including mitochondria, endoplasmic reticulum, Golgi apparatus and lysosomes according to restored fluorescence intensity. In conclusion, Ax might provide an alternative for ameliorating the oocyte quality following aging in vitro, through the mechanisms mediated by its antioxidant properties.

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Section: Discussionsupporting

confidence: 91%

Inhibitory effects of astaxanthin on postovulatory porcine oocyte aging in vitro

Jia

Xiang

Shao

et al. 2020

Sci Rep

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“…The maturation rate reported in the 0.6 mg/mL LC supplemented group was also higher than those demonstrated previously when dog oocytes cultured in vitro for 72 h (0.0% to 6.82% [ 1 ], 25.5% [ 4 ], 2.48% to 13.23% [ 43 ], 5.56% to 21.82% [ 44 ], and 3.8% [ 45 ]). Our findings suggest that LC could positively impact oocyte maturation during a prolonged culture period [ 25 , 46 ]. Regarding the effect of LC on fertilization status following IVF, our results show that treatment of oocytes with 0.6 mg/mL LC during IVM improved fertilization rates compared with the control group and other LC concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…The morula percentage reported in [ 9 ] is lower than that reported here in a 0.6 mg/mL LC supplemented group (4.5%). It is known that culturing oocytes for a long period increases ROS accumulation and reduces the antioxidant defense mechanism within the oocytes, creating oxidative stress that harmfully impacts oocyte developmental potential [ 25 , 46 ]. This notion could explain the low rates of embryo development reported in the oocytes matured for 72 h in the study of Otoi et al [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…In camels, LC improved nuclear maturation, fertilization, and blastocyst rates after IVM/IVF and embryo culture [ 24 ]. In cattle, LC promoted relocation of active mitochondria to the inner oocyte membrane and subsequently enhanced embryonic development [ 25 ]. However, there has been no analysis of the importance of LC concerning canine oocytes’ metabolism and development.…”

Section: Introductionmentioning

confidence: 99%

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Beneficial Effects of L-Carnitine Supplementation during IVM of Canine Oocytes on Their Nuclear Maturation and Development In Vitro

Moawad

Salama

Badr

et al. 2021

Animals

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This study aimed to investigate the effect of L-carnitine (LC) supplementation during in vitro maturation (IVM) of canine oocytes on nuclear maturation, fertilization status, and preimplantation development. Cumulus–oocyte complexes (COCs) collected from the ovaries of ovariohysterectomized female dogs were matured in vitro for 72 h in a TCM-199 medium supplemented with (0.1, 0.3, 0.6, 1.0, or 2.0 mg/mL) or without (0.0 mg/mL) LC. Matured oocytes were fertilized in vitro with frozen–thawed spermatozoa, and zygotes were cultured in a SOF medium for 7 days. IVM rates were higher (p ≤ 0.05) in 0.3 and 0.6 mg/mL LC supplemented groups than in the control (0.0 mg/mL LC) and other LC groups. Fertilization (18 h postinsemination (pi)) and cleavage (2–16-cell stage at day 3 pi) rates were higher (p ≤ 0.05) in the 0.6 mg/mL LC group than in the control and 0.1, 1.0, and 2 mg/mL LC supplemented groups. Interestingly, 4.5% of fertilized oocytes developed to morula (day 5 pi) in the 0.6 mg/mL LC group, which was higher (p ≤ 0.05) than those developed in the 0.3 mg/mL group (1.0%). No cleaved embryos developed to morula in other groups. In conclusion, LC supplementation at 0.6 mg/mL during IVM of canine oocytes improved their maturation, fertilization, and preimplantation embryo development rates following IVF and in vitro culture (IVC).

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“…The cleavage stage and the 4-cell stage are key stages in porcine embryonic development and zygotic genome activation [ 24 ]. Appropriate additives can be beneficial for overcoming blocks in embryonic development and for improving embryonic developmental potential [ 11 , 25 , 26 ]. Therefore, we also investigated the effects of CA on 4-cell-stage embryonic development.…”

Section: Discussionmentioning

confidence: 99%

Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species

Peng

Chen

Luo

et al. 2020

Journal of Reproduction and Development

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Carnosic acid (CA), a natural catechol rosin diterpene, is used as an additive in animal feeds and human foods. However, the effects of CA on mammalian reproductive processes, especially early embryonic development, are unclear. In this study, we added CA to parthenogenetically activated porcine embryos in an in vitro culture medium to explore the influence of CA on apoptosis, proliferation, blastocyst formation, reactive oxygen species (ROS) levels, glutathione (GSH) levels, mitochondrial membrane potential, and embryonic development-related gene expression. The results showed that supplementation with 10 μM CA during in vitro culture significantly improved the cleavage rates, blastocyst formation rates, hatching rates, and total numbers of cells of parthenogenetically activated porcine embryos compared with no supplementation. More importantly, supplementation with CA also improved GSH levels and mitochondrial membrane potential, reduced natural ROS levels in blastomeres, upregulated Nanog , Sox2 , Gata4 , Cox2 , Itga5, and Rictor expression, and downregulated Birc5 and Caspase3 expression. These results suggest that CA can improve early porcine embryonic development by regulating oxidative stress. This study elucidates the effects of CA on early embryonic development and their potential mechanisms, and provides new applications for improving the quality of in vitro -developed embryos.

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L-carnitine prevents bovine oocyte aging and promotes subsequent embryonic development

Cited by 25 publications

References 58 publications

Inhibitory effects of astaxanthin on postovulatory porcine oocyte aging in vitro

Inhibitory effects of astaxanthin on postovulatory porcine oocyte aging in vitro

Beneficial Effects of L-Carnitine Supplementation during IVM of Canine Oocytes on Their Nuclear Maturation and Development In Vitro

Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species

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