Lambda and T4 bacteriophage hybrids

Tikhomirova, L. P.; Vorozheikina, D; Putintceva, Nina I.; Matvienko, N.I.

doi:10.1016/0022-2836(77)90239-x

Cited by 13 publications

(1 citation statement)

References 12 publications

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“…Unusual resistance to endonucleolytic cleavage has been noted also in the case of EcoRI digestion of Glc-hydroxymethylated Cyt-DNA (25). T4 DNA from a pseudorevertant of ANB5060(rll)amC87(42)amE51(56) was found to be cleaved by XhoI to 15 fragments and by EcoRI and HindIII to about 60 fragments, but not by BamHI (28).…”

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confidence: 98%

Cleavage map of bacteriophage T4 cytosine-containing DNA by sequence-specific endonucleases SalI and KpnI

Carlson¹,

Nicolaisen²

1979

J Virol

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Section: Downloaded Frommentioning

confidence: 98%

Cleavage map of bacteriophage T4 cytosine-containing DNA by sequence-specific endonucleases SalI and KpnI

Carlson¹,

Nicolaisen²

1979

J Virol

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Molecular cloning of fragments of bacteriophage T4 DNA

1977

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Non-glucosylated T4 DNA was digested with R.EcoRI and the resulting fragments covalently joined to lambda vectors. The genetic content of each lambda-T4 hybrid was determined by marker-rescue tests. The isolation of many recombinants containing partial-digestion products of T4 DNA provided the overlapping sequences necessary to order fragments within the T4 genome. The present analyses include parts of the "early" region between genes 42 and 46, and much of the "late" region between genes 50 and 29. T4 cytosine-DNA digested to completion by R.EcoRI was used to identify the fragments of DNA within the lambda-T4 recombinants. The T4 cytosine-DNA was also sensitive to R.HindIII and R.Xho but not to R.BamH1.

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Complementation of T4 phage am mutations by hybrid phages λ-T4

et al. 1980

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EcoRI fragments of the T4 cytosine-containing DNA (dC-DNA) were cloned in the lambda XIII vector phage carrying the only restriction site for EcoRI in the repressor gene of lambda. 38 genes of T4 were identified in the cloned fragments by means of marker rescue technique. All cloned early genes and some late genes of T4 were able to complement a corresponding am mutations of T4 phage when nonpermissive cells of E.coli were simultaneously infected with hybrid phages lambda-T4 and am mutants of T4. An average burst size for am mutants from su- cells (when complemented by corresponding hybrid phage) was 20-70 pfu for early genes and 1-3 to 20 pfu for late ones. When the extract of cells infected with hybrid phage lambda-T4-22 containing T4 genes 57, 1, 2, 64 was mixed with the extract of cells infected with T4N51 mutant, the complementation in vitro was observed. So, it was shown that normal product of late gene 2 is synthesized in the cells infected with hybrid phage lambda-T4-22 in the absence of positive regulators of transcription coded by early T4 genes.

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Lambda and T4 bacteriophage hybrids

Cited by 13 publications

References 12 publications

Cleavage map of bacteriophage T4 cytosine-containing DNA by sequence-specific endonucleases SalI and KpnI

Cleavage map of bacteriophage T4 cytosine-containing DNA by sequence-specific endonucleases SalI and KpnI

Molecular cloning of fragments of bacteriophage T4 DNA

Complementation of T4 phage am mutations by hybrid phages λ-T4

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