Lactoferrin Blocks the Initial Host Cell Attachment Mechanism of Enteropathogenic E. Coli (EPEC)

Ochoa, Theresa J.; Noguera-Obenza, Marita; Cleary, Thomas G.

doi:10.1007/978-1-4757-4242-8_65

Cited by 11 publications

(5 citation statements)

References 9 publications

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“…This leads to the disruption and permeabilization of the bacterial membrane and ultimately cell death. Furthermore, the N-terminus of lactoferrin possesses a serine protease-like activity, which potentially degrades secreted proteins 78 .…”

Section: Antimicrobial Activity Of Lactoferrinmentioning

confidence: 99%

Lactoferrin: A Critical Mediator of Both Host Immune Response and Antimicrobial Activity in Response to Streptococcal Infections

Francis

Doster

et al. 2020

ACS Infect. Dis.

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Streptococcal species are gram positive bacteria responsible for a variety of disease outcomes including pneumonia, meningitis, endocarditis, erysipelas, necrotizing fasciitis, periodontitis, skin and soft tissue infections, chorioamnionitis, premature rupture of membranes, preterm birth, and neonatal sepsis. In response to streptococcal infections, the host innate immune system deploys a repertoire of antimicrobial and immune modulating molecules. One important molecule that is produced in response to streptococcal infections is lactoferrin. Lactoferrin has antimicrobial properties including the ability to bind iron with high affinity and sequester this important nutrient from an invading pathogen. Additionally, lactoferrin has the capacity to alter the host inflammatory response and contribute to disease outcome. This review presents the most recent published work that studies the interaction between the host innate immune protein lactoferrin and the invading pathogen, Streptococcus.

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Section: Antimicrobial Activity Of Lactoferrinmentioning

confidence: 99%

Lactoferrin: A Critical Mediator of Both Host Immune Response and Antimicrobial Activity in Response to Streptococcal Infections

Francis

Doster

et al. 2020

ACS Infect. Dis.

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“…Recombinant human lactoferrin causes loss and degradation of E. coli secreted proteins A, B and D (EspABD), especially EspB. These secreted proteins are components of the type III machinery and are known to be key elements in EPEC pathogenesis [41]. EspB plays a central role in pathogenesis both by its membrane lytic and its myosin-binding functions.…”

Section: Enteropathogenic E Coli (Epec)mentioning

confidence: 99%

Effect of lactoferrin on enteric pathogens

Ochoa

Cleary²

2009

Biochimie

118

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Much has been learned in recent years about the mechanisms by which breastfeeding improves child health and survival. However, there has been little progress in using these insights to improve pediatric care. Factors that are important for protecting the breast fed infant might be expected to decrease the adverse effects of weaning on diarrhea, growth, and development. Lactoferrin, an ironbinding protein with multiple physiological functions (anti-microbial, anti-inflammatory, and immunomodulatory), is one of the most important proteins present in mammalian milk. Protection against gastroenteritis is the most likely biologically relevant activity of lactoferrin. Multiple in vitro and animal studies have shown a protective effect of lactoferrin on infections with enteric microorganisms, including rotavirus, Giardia, Shigella, Salmonella and the diarrheagenic Escherichia coli. Lactoferrin has two major effects on enteric pathogens: it inhibits growth and it impairs function of surface expressed virulence factors thereby decreasing their ability to adhere or to invade mammalian cells. Thus, lactoferrin may protect infants from gastrointestinal infection by preventing the attachment by enteropathogens in the gut. Recently several clinical trials in children have started to address this issue. Whether lactoferrin can prevent a significant portion of diarrheal disease remains to be determined.

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“…They observed that Lf treatment of Shigella fl exneri 5 strain M90T impaired invasiveness [60] by inducing release and degradation of invasion plasmid antigens B and C (IpaB and IpaC) [61,62]. They also found that Lf blocks Enteropathogenic E. coli adherence, hemolysis and induction of actin polymerisation in HEp2 cells as a result of the Lf-mediated degradation of E. coli secreted proteins A, B and D (EspABD) [63,64]. Massucci et al [65] characterised in vitro the proteolytic activity of bovine Lf towards synthetic substrates.…”

Section: Antibacterial Activity Related To Proteolysismentioning

confidence: 92%

Lactoferrin

Valenti

Antonini

2005

Cell. Mol. Life Sci.

409

165

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The first function attributed to lactoferrin (Lf), an iron binding protein belonging to the non-immune natural defences, was antimicrobial activity that depended on its capacity to sequester iron. Iron-independent microbicidal activities, requiring direct interaction between this cationic protein and microbial surface components, were later demonstrated. Many other anti-microbial and anti-viral functions have since been ascribed to Lf. In mucosal secretions, iron and Lf modulate the motility and aggregation of pathogenic bacteria. Lf inhibits bacterial adhesion on abiotic surfaces through ionic binding to biomaterials, or specific binding to bacterial structures or both. Lf inhibition of bacterial adhesion to host cells requires Lf binding to bacteria and/or host cells. Lf hinders microbial internalization by binding to both glycosaminoglycans and bacterial proteins which can be degraded by Lf-mediated proteolysis. Moreover, Lf internalisation and localisation to the host cell nuclei could modulate bacterial entry into cells through gene regulation. Finally, the capability of Lf to exert antiviral activity, through its binding to host cells and/or viral particles, strengthens the idea that it is an important brick in the mucosal wall, effective against both microbial and viral attacks.

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Lactoferrin Blocks the Initial Host Cell Attachment Mechanism of Enteropathogenic E. Coli (EPEC)

Cited by 11 publications

References 9 publications

Lactoferrin: A Critical Mediator of Both Host Immune Response and Antimicrobial Activity in Response to Streptococcal Infections

Lactoferrin: A Critical Mediator of Both Host Immune Response and Antimicrobial Activity in Response to Streptococcal Infections

Effect of lactoferrin on enteric pathogens

Lactoferrin

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