Lactoferrin Adsorbed onto Biomimetic Hydroxyapatite Nanocrystals Controlling - In Vivo - the Helicobacter pylori Infection

Fulgione, Andrea; Nocerino, Nunzia; Iannaccone, Marco; Roperto, Sante; Capuano, Federico; Roveri, Norberto; Lelli, Marco; Crasto, Antonio; Calogero, Armando; Pilloni, Argenia Paola; Capparelli, Rosanna

doi:10.1371/journal.pone.0158646

Cited by 23 publications

(21 citation statements)

References 38 publications

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“…The high reactivity of HA is ascribed to its amorphous surface 16 and to its high surface area of about 110 m 2 /g, which is only slightly lower than that of biological nanocrystals (120 m 2 /g). 30 Moreover, the degree of crystallinity and the presence of carbonate ions in the structure of biomimetic HA (data not shown) clearly confirm that the HA selected for this study is -structurally -very similar to that of the bone, not only in the size of the nanocrystals but also in ionic substitution.…”

Section: Biomimetic Ha Nanocrystal Characterizationsupporting

confidence: 55%

“…[24][25][26][27][28][29] Furthermore, HA nanocrystals have low toxicity and remarkable stability, as reported in our previous studies. 30 In industrialized countries, infections acquired by food and water represent a big concern for public health, and about 50% of foodborne infections in humans are caused by Salmonella spp. 31 Despite control measures and monitoring carried out by the healthcare authorities, cases of Salmonella contaminations are still very frequent.…”

Section: Introductionmentioning

confidence: 99%

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Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages

Fulgione

Ianniello

Papaianni

et al. 2019

IJN

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The use of bacteriophages represents a valid alternative to conventional antimicrobial treatments, overcoming the widespread bacterial antibiotic resistance phenomenon. In this work, we evaluated whether biomimetic hydroxyapatite (HA) nanocrystals are able to enhance some properties of bacteriophages. The final goal of this study was to demonstrate that biomimetic HA nanocrystals can be used for bacteriophage delivery in the context of bacterial infections, and contribute-at the same time-to enhance some of the biological properties of the same bacteriophages such as stability, preservation, antimicrobial activity, and so on. Materials and methods: Phage isolation and characterization were carried out by using Mitomycin C and following double-layer agar technique. The biomimetic HA water suspension was synthesized in order to obtain nanocrystals with plate-like morphology and nanometric dimensions. The interaction of phages with the HA was investigated by dynamic light scattering and Zeta potential analyses. The cytotoxicity and intracellular killing activities of the phage-HA complex were evaluated in human hepatocellular carcinoma HepG2 cells. The bacterial inhibition capacity of the complex was assessed on chicken minced meat samples infected with Salmonella Rissen. Results: Our data highlighted that the biomimetic HA nanocrystal-bacteriophage complex was more stable and more effective than phages alone in all tested experimental conditions. Conclusion: Our results evidenced the important contribution of biomimetic HA nanocrystals: they act as an excellent carrier for bacteriophage delivery and enhance its biological characteristics. This study confirmed the significant role of the mineral HA when it is complexed with biological entities like bacteriophages, as it has been shown for molecules such as lactoferrin.

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Section: Biomimetic Ha Nanocrystal Characterizationsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Biomimetic hydroxyapatite nanocrystals are an active carrier for Salmonella bacteriophages

Fulgione

Ianniello

Papaianni

et al. 2019

IJN

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“…Fulgione et al also demonstrated that the better performance of lactoferrin delivered by biomimetic hydroxyapatite, and the complex can act as a treatment for bacteria infection [48] .…”

Section: Cell Viabilitymentioning

confidence: 99%

Hydroxyapatite nanorod and microsphere functionalized with bioactive lactoferrin as a new biomaterial for enhancement bone regeneration

Shi

Wang

et al. 2017

Colloids and Surfaces B: Biointerfaces

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“…Thus, different studies have been performed with Lf-HA functionalized nanocrystals [50,51] revealing this combination as a promising system with anti-inflammatory properties and increased osteogenic capacity. It has been proved that biomimetic HA nanocrystals surface-functionalized with Lf have antibacterial activity effective against Gram-positive and Gram-negative bacteria [52][53][54].…”

Section: Of 19mentioning

confidence: 99%

Macrophage in vitro Response on Hybrid Coatings Obtained by Matrix Assisted Pulsed Laser Evaporation

et al. 2019

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The improvement in the research area of the implant by surface functionalization when correlated with the biological response is of major interest in the biomedical field. Based on the fact that the inflammatory response is directly involved in the ultimate response of the implant within the body, it is essential to study the macrophage-material interactions. Within this context, we have investigated the composite material-macrophage cell interactions and the inflammatory response to these composites with amorphous hydroxyapatite (HA), Lactoferrin (Lf), and polyethylene glycol-polycaprolactone (PEG-PCL) copolymer. All materials are obtained by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique and characterized by Atomic Force Microscopy and Scanning Electron Microscopy. Macrophage-differentiated THP-1 cells proliferation and metabolic activity were assessed by qualitative and quantitative methods. The secretion of tumor necrosis factor alpha (TNF-α) and interleukin 10 (IL-10) cytokine, in the presence and absence of the inflammatory stimuli (bacterial endotoxin; lipopolysaccharide (LPS)), was measured using an ELISA assay. Our results revealed that the cellular response depended on the physical-chemical characteristics of the coatings. Copolymer-HA-Lf coatings led to low level of pro-inflammatory TNF-α, the increased level of anti-inflammatory IL-10, and the polarization of THP-1 cells towards an M2 pro-reparative phenotype in the presence of LPS. These findings could have important potential for the development of composite coatings in implant applications.

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Lactoferrin Adsorbed onto Biomimetic Hydroxyapatite Nanocrystals Controlling - In Vivo - the Helicobacter pylori Infection

Cited by 23 publications

References 38 publications

<p>Biomimetic hydroxyapatite nanocrystals are an active carrier for <em>Salmonella</em> bacteriophages</p>

<p>Biomimetic hydroxyapatite nanocrystals are an active carrier for <em>Salmonella</em> bacteriophages</p>

Hydroxyapatite nanorod and microsphere functionalized with bioactive lactoferrin as a new biomaterial for enhancement bone regeneration

Macrophage in vitro Response on Hybrid Coatings Obtained by Matrix Assisted Pulsed Laser Evaporation

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