Lactococcus lactis as an Interleukin Delivery System for Prophylaxis and Treatment of Inflammatory and Autoimmune Diseases

Campos, Gabriela Munis; Américo, Monique Ferrary; Freitas, Andria dos Santos; Barroso, Fernanda Alvarenga Lima; Dutra, Joyce da Cruz Ferraz; Quaresma, Ludmila Silva; Cordeiro, Bárbara Fernandes; Laguna, Juliana; Jesus, Luís Cláudio Lima de; Fontes, Aparecida Maria; Birbrair, Alexander; Santos, Túlio M.; Azevedo, Vasco

doi:10.1007/s12602-023-10041-1

Cited by 4 publications

(1 citation statement)

References 143 publications

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“…However, our data from viability in SHIME growth media, suggest that the Lc4 strain could survive and retain enzymatic activity in the GI tract. L. lactis strains and other GRAS bacteria, therefore, which are resistant to the mentioned environmental factors, could be promising alternatives even for high molecular mass proteins vulnerable to digestion [128,129]. Several studies have demonstrated that large proteins, native or heterologously expressed, could be active and impart beneficial effects on the host in vivo via the digestive tract [52][53][54]130].…”

Section: Discussionmentioning

confidence: 99%

The strain-dependent cytostatic activity of Lactococcus lactis on CRC cell lines is mediated through the release of arginine deiminase

Jastrząb,

Tomecki,

Jurkiewicz

et al. 2024

Microb Cell Fact

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Background Colorectal cancer (CRC) is one of the most commonly diagnosed cancers, posing a serious public health challenge that necessitates the development of new therapeutics, therapies, and prevention methods. Among the various therapeutic approaches, interventions involving lactic acid bacteria (LAB) as probiotics and postbiotics have emerged as promising candidates for treating and preventing CRC. While human-isolated LAB strains are considered highly favorable, those sourced from environmental reservoirs such as dairy and fermented foods are also being recognized as potential sources for future therapeutics. Results In this study, we present a novel and therapeutically promising strain, Lactococcus lactis ssp. lactis Lc4, isolated from dairy sources. Lc4 demonstrated the ability to release the cytostatic agent - arginine deiminase (ADI) - into the post-cultivation supernatant when cultured under conditions mimicking the human gut environment. Released arginine deiminase was able to significantly reduce the growth of HT-29 and HCT116 cells due to the depletion of arginine, which led to decreased levels of c-Myc, reduced phosphorylation of p70-S6 kinase, and cell cycle arrest. The ADI release and cytostatic properties were strain-dependent, as was evident from comparison to other L. lactis ssp. lactis strains. Conclusion For the first time, we unveil the anti-proliferative properties of the L. lactis cell-free supernatant (CFS), which are independent of bacteriocins or other small molecules. We demonstrate that ADI, derived from a dairy-Generally Recognized As Safe (GRAS) strain of L. lactis, exhibits anti-proliferative activity on cell lines with different levels of argininosuccinate synthetase 1 (ASS1) expression. A unique feature of the Lc4 strain is also its capability to release ADI into the extracellular space. Taken together, we showcase L. lactis ADI and the Lc4 strain as promising, potential therapeutic agents with broad applicability.

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Section: Discussionmentioning

confidence: 99%

The strain-dependent cytostatic activity of Lactococcus lactis on CRC cell lines is mediated through the release of arginine deiminase

Jastrząb,

Tomecki,

Jurkiewicz

et al. 2024

Microb Cell Fact

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The potential use of bacteria and bacterial derivatives as drug delivery systems for viral infection

Faghihkhorasani,

Ahmed,

Mashool

et al. 2023

Virol J

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Viral infections in humans are responsible for fatalities worldwide and contribute to the incidence of various human ailments. Controllable targeted medicine delivery against many illnesses, including viral infection, may be significantly aided by using bacteria and bacteria-derived products. They may accumulate in diseased tissues despite physical obstacles, where they can launch antiviral immunity. The ability to genetically and chemically modify them means that vaccinations against viral infections may be manufactured and delivered to affected tissues more safely and effectively. The objective of this study is to provide an overview of the latest advancements in the field of utilizing bacteria and bacterial derivatives as carriers for administering medication to treat viral diseases such as SARS-CoV-2, hepatitis B virus, hepatitis C virus, human immunodeficiency virus, human papillomavirus, influenza, and Ebola virus. Graphical Abstract

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Probiotic Characteristics of Lactic Acid Bacteria Isolated From Sui Wu’u: A Traditional Food from Bajawa, West Flores, Indonesia

Rubak,

J.D. Lalel,

Sanam

et al. 2023

Curr Res Nutr Food Sci

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The probiotic potential of lactic acid bacteria originating from traditional food will always be a concern for researchers, along with increasing public awareness of health. One source of LAB isolates with probiotic potential is traditional food. This study aims to investigate the probiotic potential of lactic acid bacteria from Sui Wu’u, a traditional food-based pork originating from Bajawa, East Nusa Tenggara, Indonesia. Sui Wu’u was prepared based on method of the Bajawa community and was analyzed after being stored for six months. Total LAB, morphological, and phenotypic identification was carried out as well as testing the ability of isolates to ferment sugar. Probiotics potency was tested on resistance to low pH and gastric acid, resistance to pathogenic bacteria, and exposure to antibiotics. The results indicated that LAB isolated from Sui Wu’u were dominated by the coci form. The four selected isolates had resistance with a survival rate of 88.3%–96.88% when exposed to pH 2.5 and a survival rate of 98.69%–99.03% when exposed to bile salts at 0.3%. Moderate resistance was exhibited by all isolates against E. coli, S. typhimurium, and S. aureus, while no resistance was observed against B. cereus. All strains demonstrated sensitivity to tetracycline, ampicillin, and chloramphenicol but exhibited resistant to streptomycin. All four isolates were identified as Lactococcus lactis. These results show that four strains of Sui Wu’u have demonstrated their potential as probiotics.

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Lactococcus lactis as an Interleukin Delivery System for Prophylaxis and Treatment of Inflammatory and Autoimmune Diseases

Cited by 4 publications

References 143 publications

The strain-dependent cytostatic activity of Lactococcus lactis on CRC cell lines is mediated through the release of arginine deiminase

The strain-dependent cytostatic activity of Lactococcus lactis on CRC cell lines is mediated through the release of arginine deiminase

The potential use of bacteria and bacterial derivatives as drug delivery systems for viral infection

Probiotic Characteristics of Lactic Acid Bacteria Isolated From Sui Wu’u: A Traditional Food from Bajawa, West Flores, Indonesia

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