Lactobacillus sps. lipase mediated poly (ε-caprolactone) degradation

Khan, Imran; Dutta, Jayati Ray; Ganesan, Ramakrishnan

doi:10.1016/j.ijbiomac.2016.11.040

Cited by 57 publications

(40 citation statements)

References 35 publications

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“…Lipase was extracted from L. plantarum and purified using the previously reported literature. 31 The change in the percentage crystallinity and thermal mass loss behavior of the samples were studied by differential thermal and thermogravimetric analyses using Shimadzu DTG-60. For this, the enzyme-embedded PCL films were subjected to heating in a nitrogen atmosphere from 35 to 600 °C at a heating rate of 10 °C/min under a steady nitrogen flow rate of 20 mL/min.…”

Section: Methodsmentioning

confidence: 99%

Enzyme-Embedded Degradation of Poly(ε-caprolactone) using Lipase-Derived from Probiotic Lactobacillus plantarum

et al. 2019

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Enzyme-embedded polymer degradation was reported to be an attractive alternative approach to the conventional surface pouring method for efficient degradation of polymers using fungal-derived enzyme Candida antarctica lipase B. Despite the enormous potential, this approach is still in its infancy. In the present study, a probiotic lipase obtained from Lactobacillus plantarum has been employed for the first time to study the enzyme-embedded polymer degradation approach using poly(ε-caprolactone) (PCL) as the semicrystalline polymer candidate. PCL films embedded with 2 to 8 wt % lipase are studied under static conditions for their enzymatic degradation up to 8 days of incubation. Thermogravimetric analyses (TGA) have shown a clear trend in decreasing thermal stability of the polymer with increasing lipase content and number of incubation days. Differential thermal analyses have revealed that the percentage crystallinity of the leftover PCL films increases with progress in enzymatic degradation because of the efficient action of lipase over the amorphous regions of the films. Thus, the higher lipase loading in the PCL matrix and more number of incubation days have resulted in higher percentage crystallinity in the leftover PCL films, which has further been corroborated by X-ray diffraction analyses. In a similar line, higher percentage mass loss of the PCL films has been observed with increased enzyme loading and number of incubation days. Field emission scanning electron microscopy (FE-SEM) has been employed to follow the surface and cross-sectional morphologies of the polymer films, which has revealed micron-scale pores on the surface as well as a bulk polymer matrix with progress in enzymatic polymer degradation. Additionally, FE-SEM studies have revealed the efficient enzyme-catalyzed hydrolysis of the polymer matrix in a three-dimensional fashion, which is unique to this approach. In addition to the first-time utility of a probiotic lipase for the embedded polymer degradation approach, the present work provides insight into the PCL degradation under static and ambient temperature conditions with no replenishment of enzymes.

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Section: Methodsmentioning

confidence: 99%

Enzyme-Embedded Degradation of Poly(ε-caprolactone) using Lipase-Derived from Probiotic Lactobacillus plantarum

et al. 2019

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“…Lipase from Lactobacillus plantarum (MTCC 4461) was isolated as per our previous reports. 26,27 Silver Nitrate, NaBH 4 and PBS buffer (pH 7.4) used were purchased from Sigma Aldrich. Pseudomonas putida, Staphylococcus aureus and Aspergillus niger culture were obtained from MTCC, Chandigarh, India.…”

Section: Methodsmentioning

confidence: 99%

Extracellular probiotic lipase capped silver nanoparticles as highly efficient broad spectrum antimicrobial agents

et al. 2018

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“…The strain showed resemblance to Pseudomonas pachastrellae. The lipase enzyme from Lactobacillus brevis , Lactobacillus plantarum and their co‐culture was reported to degrade PCL film (Khan et al 2017). Another study conducted in Taiwan reported Streptomyces thermoviolaceus subsp .…”

Section: Microbial Degradation Of Polyester‐based Biodegradable Plasticsmentioning

confidence: 99%

Polyester‐based biodegradable plastics: an approach towards sustainable development

Satti

Shah

2020

Lett Appl Microbiol

108

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Significance and Impact of the Study: Polyester-based BPs considered as a solution to current plastic waste problem as well as leading polymers in terms of biodegradability and sustainability has been critically discussed. The role of microorganisms and their enzymes involved in the biodegradation of these polymers and ecotoxicological impact of degradation products of BPs on soil microbial community and biogeochemical cycles has also been described. This report will provide an insight on the key research areas to bridge the gap for development of simulated systems as an effective and emerging strategy to divert the overflow of plastic in the environment as well as for the greener solution to the plastic waste management problems. AbstractNon-degradability of conventional plastics, filling of landfill sites, raising water and land pollution and rapid depletion of fossil resources have raised the environmental issues and global concerns. The current demand and production of plastics is putting immense pressure on fossil resources, consuming about 6% of the global oil and is expected to grow up to 20%. The polyester-based biodegradable plastics (BPs) are considered as a remedy to the issue of plastics waste in the environment. BPs appear to manage the overflow of plastics by providing new means of waste management system and help in securing the non-renewable resources of nature. This review comprehensively presents the environmental burdens due to conventional plastics as well as production of polyester-based BPs as an alternative to conventional commodity plastics. The diversity of micro-organisms and their enzymes that degrade various polyesterbased BPs (PLA, PCL, PHB/PHBV and PET) has also been described in detail. Moreover, the impact of plastics degradation products on soil ecology and ecosystem functions has critically been discussed. The report ends with special focus on future recommendations for the development of sustainable waste management strategies to control pollution due to plastics waste. Letters in Applied Microbiology ISSN 0266-8254Packaging, compost bags and agricultural mulching films Li et al. (2015) Letters in Applied Microbiology 70, 413--430

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Lactobacillus sps. lipase mediated poly (ε-caprolactone) degradation

Cited by 57 publications

References 35 publications

Enzyme-Embedded Degradation of Poly(ε-caprolactone) using Lipase-Derived from Probiotic Lactobacillus plantarum

Enzyme-Embedded Degradation of Poly(ε-caprolactone) using Lipase-Derived from Probiotic Lactobacillus plantarum

Extracellular probiotic lipase capped silver nanoparticles as highly efficient broad spectrum antimicrobial agents

Polyester‐based biodegradable plastics: an approach towards sustainable development

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