Lactobacillus salivarius LI01 encapsulated in alginate-pectin microgels ameliorates d-galactosamine-induced acute liver injury in rats

Zhuge, Aoxiang; Li, Bo; Yin, Yuan; Lv, Longxian; Li, Yating; Wu, Jingjing; Yang, Liya; Bian, Xiaoyuan; Wang, Kaicen; Wang, Qiangqiang; Ren, Yan; Zhu, Xueling; Li, Lanjuan

doi:10.1007/s00253-020-10749-y

Cited by 34 publications

(24 citation statements)

References 92 publications

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“…The ten OTUs more abundant and prevalent in the healthy inocula were Ruminococcaceae UCG-004, Ruminococcaceae UCG-014, Bacteroides, Alistipes, Desulfovibrio, Bilophila and Parabacteroides. In the literature, Ruminococcaceae UCG-004 seems to be considered as a pathogenic taxon, whereas Ruminococcaceae UCG-014 is considered as a beneficial one [43]. Bacteroides ovatus was found to exert protective effects against the development of metabolic disorders in mice [14].…”

Section: Discussionmentioning

confidence: 99%

Fecal Microbiota Transplant from Human to Mice Gives Insights into the Role of the Gut Microbiota in Non-Alcoholic Fatty Liver Disease (NAFLD)

et al. 2021

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Non-alcoholic fatty liver diseases (NAFLD) are associated with changes in the composition and metabolic activities of the gut microbiota. However, the causal role played by the gut microbiota in individual susceptibility to NAFLD and particularly at its early stage is still unclear. In this context, we transplanted the microbiota from a patient with fatty liver (NAFL) and from a healthy individual to two groups of mice. We first showed that the microbiota composition in recipient mice resembled the microbiota composition of their respective human donor. Following administration of a high-fructose, high-fat diet, mice that received the human NAFL microbiota (NAFLR) gained more weight and had a higher liver triglycerides level and higher plasma LDL cholesterol than mice that received the human healthy microbiota (HR). Metabolomic analyses revealed that it was associated with lower and higher plasma levels of glycine and 3-Indolepropionic acid in NAFLR mice, respectively. Moreover, several bacterial genera and OTUs were identified as differently represented in the NAFLR and HR microbiota and therefore potentially responsible for the different phenotypes observed. Altogether, our results confirm that the gut bacteria play a role in obesity and steatosis development and that targeting the gut microbiota may be a preventive or therapeutic strategy in NAFLD management.

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Section: Discussionmentioning

confidence: 99%

Fecal Microbiota Transplant from Human to Mice Gives Insights into the Role of the Gut Microbiota in Non-Alcoholic Fatty Liver Disease (NAFLD)

et al. 2021

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“…Previously, several microencapsulation methods and materials have been studied on different strains of probiotics to analyze their viability. Alginate-pectin microgel [34],…”

Section: Discussionmentioning

confidence: 99%

“…Previously, several microencapsulation methods and materials have been studied on different strains of probiotics to analyze their viability. Alginate-pectin microgel [34], chitosan-alginate [35], cellulose sulphate [36], etc., as encapsulating material protected different bacterial and fungal strains of probiotics from unfavorable gastrointestinal environment and improved the survival of bacterial cell, intestinal delivery and release resulting to several health bene ts. However, pectin encapsulated probiotics did not enhance the effect of probiotics supplementation [26].…”

Section: Discussionmentioning

confidence: 99%

Oral delivery of Lactobacillus paracasei via microcapsule modulates gut health and intestinal microbiota

Gyawali¹,

Guiyuan²,

Xu³

et al. 2021

Preprint

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Background: The beneficial impact of probiotics on host health is impaired due to the significant loss of survivability during gastric transit, small intestinal enzymes, and bile acids. Encapsulation helps to preserve the probiotics species from severe environmental factors. This study investigated the effects of oral delivery of probiotics via microcapsule on different parameters of gut health. Methods: Lactobacillus paracasei, highly sensitive probiotic species to gastric acid, was encapsulated with novel encapsulating material, polyacrylate resin, to get a microcapsule. C57BL/6 male mice were equally divided into three groups; supplementing basal feed, a mixture of encapsulating material and Lactobacillus paracasei, and encapsulated Lactobacillus paracasei (microcapsule) for four weeks. Fecal moisture percentage was measured regularly, which was elevated in the encapsulated group, but not in the mixed group. Based on this data, mice from control and encapsulated groups were sacrificed to study the different parameters of gut health. Results: Compared to control, encapsulated probiotics increased villi height, the ratio of villi height to crypt depth, and decreased crypt depth. Simultaneously, the tight junction proteins were upregulated on encapsulated group showing enhancement of intestinal barrier functions. The level of SigA and mucin increased along with gene expression of MUC-2 but, albumin level was decreased. In addition, we found a rise in the relative gene expression of anti-inflammatory factor (IL-10) and decreased pro-inflammatory factors (IL-1β, IL-6, IL-8, and TNF-α). Meanwhile, microbiota metabolites, fecal LPS and TMAO were downregulated while SCFA and lactate were upraised compared to control. Furthermore, GSH-Px and TAOC level were increased and MDA was decreased. Microbiota analysis revealed the proportion of firmicutes was higher at the phylum level on an encapsulated group, while Lactobacillus was elevated at the genus level. We also found a remarkable increase in the population of Lactobacillus murinus at the species level. In summary, the oral delivery of probiotics via microcapsule effectively improves the animals' gut health by improving morphology, barrier function, anti-inflammatory action, antioxidant ability and modulating gut microbiota.

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“…For example, Lactobacillus salivarius LI01 was reported to attenuate hepatic injury. 90 During the treatment of ALI or ALF, the use of probiotics is commonly recognized, but it brings limited survival benefits. FMT is a potential treatment strategy for ALI and ALF to regulate the gut microbiota.…”

Section: Fecal Microbiota Transplantation To Treat Microbiota Imbalances In Liver Diseasementioning

confidence: 99%

Clinical Application and Progress of Fecal Microbiota Transplantation in Liver Diseases: A Review

Qin

Zhang

et al. 2021

Semin Liver Dis

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The human gut harbors a dense and highly diverse microbiota of approximately 1,000 bacterial species. The interaction between the host and gut bacteria strongly influences human health. Numerous evidence suggest that intestinal flora imbalance is closely associated with the development and treatment of liver diseases, including acute liver injury and chronic liver diseases (cirrhosis, autoimmune liver disease, and fatty liver). Therefore, regulating the gut microbiota is expected to be a new method for the adjuvant treatment of liver diseases. Fecal microbiota transplantation (FMT) is defined as the transplantation of gut microbiota from healthy donors to sick patients via the upper or lower gastrointestinal route to restore the normal intestinal balance. In this study, we briefly review the current research on the gut microbiota and its link to liver diseases and then summarize the evidence to elucidate the clinical application and development of FMT in liver disease treatment.

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Lactobacillus salivarius LI01 encapsulated in alginate-pectin microgels ameliorates d-galactosamine-induced acute liver injury in rats

Cited by 34 publications

References 92 publications

Fecal Microbiota Transplant from Human to Mice Gives Insights into the Role of the Gut Microbiota in Non-Alcoholic Fatty Liver Disease (NAFLD)

Fecal Microbiota Transplant from Human to Mice Gives Insights into the Role of the Gut Microbiota in Non-Alcoholic Fatty Liver Disease (NAFLD)

Oral delivery of Lactobacillus paracasei via microcapsule modulates gut health and intestinal microbiota

Clinical Application and Progress of Fecal Microbiota Transplantation in Liver Diseases: A Review

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