Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: Involvement of lipid rafts

Mechoud, Mónica A.; Mateos, Melina Valeria; Valdez, Graciela Font de; Villena, Julio; Salvador, Gabriela Alejandra; Rodríguez, Ana V.

doi:10.1016/j.intimp.2012.08.020

Cited by 18 publications

(24 citation statements)

References 36 publications

(52 reference statements)

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“…We found a significant decrease in the intracellular staining of pro-inflammatory IL-12 in ex vivo unstimulated and LPS-stimulated CD14 + monocytes and a decrease in pro-inflammatory TNF-a intracellular staining in LPSstimulated CD14 + monocytes at weeks 4, 10 and 14 of LcS feeding. The reduction in CD14 + intracellular TNF-a staining is consistent with other human and animal studies showing that Lactobacillus probiotics decrease TNF-a [20,[71][72][73][74] although not all [51]. It is possible that these are some of the strategies employed by LcS to avoid host defence mechanisms.…”

Section: Discussionsupporting

confidence: 88%

Immune Response of Healthy Adults to the Ingested Probiotic Lactobacillus casei Shirota

et al. 2016

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Section: Discussionsupporting

confidence: 88%

Immune Response of Healthy Adults to the Ingested Probiotic Lactobacillus casei Shirota

et al. 2016

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“…To exemplify that, PBMC treated with Lact. reuteri CRL1098 soluble factors (Lr-S) showed a reduced production of TNF-a (Mechoud et al 2012). It is important to note that the use of PBMC is an artificial screening tool, as under normal healthy conditions, intestinal bacteria do not enter the circulation, and thus cannot communicate directly with such cells because of the 'firewall' effect guaranteed by the MLN (Macpherson and Smith 2006).…”

Section: Effects On Lymphocyte Proliferation and Peripheral Blood Monmentioning

confidence: 99%

Cross-talk between probiotic lactobacilli and host immune system

et al. 2014

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SummaryThe mechanism by which probiotic lactobacilli affect the immune system is strain specific. As the immune system is a multicompartmental system, each strain has its way to interact with it and induce a visible and quantifiable effect. This review summarizes the interplay existing between the host immune system and probiotic lactobacilli, that is, with emphasis on lactobacilli as a prototype probiotic genus. Several aspects including the bacterial-host crosstalk with the mucosal and systemic immune system are presented, as well as short sections on the competing effect towards pathogenic bacteria and their uses as delivery vehicle for antigens.

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“…Although not well understood, it is likely that the sum of bacterial cell surface-derived and soluble factors and/or exopolysaccharides (EPS) contribute to the development of different immune responses induced by L. reuteri strains. For example, soluble factors of L. reuteri CRL1098 were able to reduce TNF-α production by human PBMCs (Mechoud et al, 2012) and in mouse models (Griet et al, 2014). Furthermore, human moDCs generated in the presence of the soluble factors of L. reuteri DSM 17938 down-modulated LPS-induced IL-6, IL-10, and IL-23 secretion while the secretion of regulatory cytokine TGF-β remained unaffected (Haileselassie et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors

et al. 2017

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The vertebrate gut symbiont Lactobacillus reuteri exhibits strain-specific adhesion and health-promoting properties. Here, we investigated the role of the mucus adhesins, CmbA and MUB, upon interaction of L. reuteri ATCC PTA 6475 and ATCC 53608 strains with human monocyte-derived dendritic cells (moDCs). We showed that mucus adhesins increased the capacity of L. reuteri strains to interact with moDCs and promoted phagocytosis. Our data also indicated that mucus adhesins mediate anti- and pro-inflammatory effects by the induction of interleukin-10 (IL-10), tumor necrosis factor alpha (TNF-α), IL-1β, IL-6, and IL-12 cytokines. L. reuteri ATCC PTA 6475 and ATCC 53608 were exclusively able to induce moDC-mediated Th1 and Th17 immune responses. We further showed that purified MUB activates moDCs and induces Th1 polarized immune responses associated with increased IFNγ production. MUB appeared to mediate these effects via binding to C-type lectin receptors (CLRs), as shown using cell reporter assays. Blocking moDCs with antibodies against DC-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) or Dectin-2 did not affect the uptake of the MUB-expressing strain, but reduced the production of TNF-α and IL-6 by moDCs significantly, in line with the Th1 polarizing capacity of moDCs. The direct interaction between MUB and CLRs was further confirmed by atomic force spectroscopy. Taken together these data suggest that mucus adhesins expressed at the cell surface of L. reuteri strains may exert immunoregulatory effects in the gut through modulating the Th1-promoting capacity of DCs upon interaction with C-type lectins.

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Lactobacillus reuteri CRL1098 soluble factors modulate tumor necrosis factor alpha production in peripheral blood mononuclear cells: Involvement of lipid rafts

Cited by 18 publications

References 36 publications

Immune Response of Healthy Adults to the Ingested Probiotic Lactobacillus casei Shirota

Immune Response of Healthy Adults to the Ingested Probiotic Lactobacillus casei Shirota

Cross-talk between probiotic lactobacilli and host immune system

Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors

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