Lactobacillus reuteri 5454 and Bifidobacterium animalis ssp. lactis 5764 improve colitis while differentially impacting dendritic cells maturation and antimicrobial responses

Abstract

Crohn’s disease is linked to a decreased diversity in gut microbiota composition as a potential consequence of an impaired anti-microbial response and an altered polarization of T helper cells. Here, we evaluated the immunomodulatory properties of two potential probiotic strains, namely a Bifidobacterium animalis spp. lactis Bl 5764 and a Lactobacillus reuteri Lr 5454 strains. Both strains improved colitis triggered by either 2,4,6-trinitrobenzenesulfonic acid (TNBS) or Citrobacter rodentium infection in mice. Training of dendritic cells (DC) with Lr 5454 efficiently triggered IL-22 secretion and regulatory T cells induction in vitro, while IL-17A production by CD4+ T lymphocytes was stronger when cultured with DCs that were primed with Bl 5764. This strain was sufficient for significantly inducing expression of antimicrobial peptides in vivo through the Crohn’s disease predisposing gene encoding for the nucleotide-binding oligomerization domain, containing protein 2 (NOD2). In contrast, NOD2 was dispensable for the impact on antimicrobial peptide expression in mice that were monocolonized with Lr 5454. In conclusion, our work highlights a differential mode of action of two potential probiotic strains that protect mice against colitis, providing the rational for a personalized supportive preventive therapy by probiotics for individuals that are genetically predisposed to Crohn’s disease.

In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice

“…Bacteria-primed bone marrow derived dendritic cells (BMDCs) were differentiated from the bone marrow of BALB/c mice as described previously [ 60 , 61 ]. On day 9 after differentiation, cells were stimulated by bacteria (PF-BaE5 and PF-BaE11 strains) at a ratio bacteria/cells 10:1 for 5 h (gene expression analysis) or 24 h (flow cytometry analyses of co-stimulatory markers, co-culture with CD4 + T cells).…”

The Effectiveness of Probiotics in the Treatment of Inflammatory Bowel Disease (IBD)—A Critical Review

“…In the report, the difference between colitis models was also visible, nevertheless, it was not a subject of assessment. Another research group headed by Hrdy [ 85 ] proved that probiotic strains affected host cells in many ways. The study was based on the murine model of TNBS-induced colitis, and the mechanism of action of Bifidobacterium animalis spp.…”

The Potential of Lactobacillus spp. for Modulating Oxidative Stress in the Gastrointestinal Tract

“…Recently, Le and Yang [ 114 ] reported that IBD could be potentially prevented and managed by L. plantarum , in which the modulation of the ratio of T helper cell 1 (Th1) and T helper cell 2 (Th2) plays an important role. In addition to IBD, it has been reported that CD, which was induced by either TNBS or a mucosal pathogen Citrobacter rodentium , could be potentially ameliorated by L. reuteri in a mouse model [ 115 ]. Wang et al [ 110 ] investigated the anti-inflammatory effect of L. plantarum strain ZDY2013 and B. bifidum WBIN03 strains on dextran sodium sulphate (DSS)-induced UC in a mouse model.…”