Lactobacillus helveticus and Lactobacillus plantarum modulate renal antioxidant status in a rat model of fructose-induced metabolic syndrome

Abstract

High dietary fructose intake causes a metabolic disorder and augments the risk of chronic kidney disease most likely due to oxidative stress. Probiotics could have antioxidant, antiinflammatory and immunoregulatory properties. The present study examined the influence of Lactobacillus helveticus and Lactobacillus plantarum supplementation on dietary fructose-induced metabolic changes and renal antioxidant/oxidant status of rats. Male Wistar rats were divided into four groups as follows: control; fructose; fructose plus L. helveticus; fructose plus L. plantarum. Fructose was given to the rats as a 20% solution in drinking water for 15 weeks. The probiotic supplementation was applied by gastric gavage once a day for six weeks. Several metabolic parameters in the plasma, gene and protein expressions of the main antioxidant enzymes in renal tissues of rats were measured. Dietary fructose-induced elevations in plasma insulin, triglyceride, VLDL, creatinine as well as renal urea levels were alleviated after treatment with L. helveticus and L. plantarum. Moreover, L. helveticus and L. plantarum supplementation recovered the changes in renal protein expression level of SOD1, SOD2 and CAT. In conclusion, supplementation with L. helveticus and L. plantarum has an improving effect on specific metabolic parameters and renal antioxidative enzymes in a fructose-induced metabolic disorder.

The regulatory effects of resveratrol on the expression of renal MMP-2 and MMP-9 in the rat models of diabetes.

“…In acute or chronic kidney damage that develops with experimental diabetes models created in animals; It has been shown that plasma creatinine, CK, albumin, cystatin-C, urea and uric acid levels increase. (Yuko, 2013;Korkmaz, 2019;Safrida, 2019). In this study, a significant increase showed in the levels of renal CK, urea, and uric acid.…”

“…Another unfavorable alter triggered by diabetes is the increase of oxidative stress. Numerous studies demonstrated that increased oxidative end products such as malondialdehyde (MDA) in tissues of rats with diabetes (Pektas, 2016b; whereas reduced antioxidant enzyme activities such as SOD, CAT, glutathione (GSH), and glutathione peroxidase (GPx) Korkmaz, 2019). However, nitrite/nitrate, which is the end-products of nitric oxide known as tissueprotective, has been found to decrease with diabetes (Akar, 2011).…”

Effects of Lactobacillus Plantarum and Lactobacillus Helveticus on Renal Insulin Signaling, Inflammatory Markers, and Glucose Transporters in High-Fructose-Fed Rats

“…The data with metabolic parameters, including body weight, caloric intake, the plasma level of glucose, insulin, and triglyceride, in rats upon fructose feeding, as well as L. plantarum and L. helveticus supplementations, have been published in our very recent study [26]. Briefly, in that paper, we have shown that intervention with dietary high-fructose or two probiotics did not change the body weight of rats.…”

“…A limited number of studies, mentioned above, demonstrates the need for mechanistic further studies with probiotics in high-fructose-induced metabolic syndrome. Recently, we have shown that the dietary fructose induced the changes in some metabolic parameters, such as plasma insulin, triglyceride, VLDL, creatinine, and renal urea, as well as in renal protein expression level of superoxide dismutase 1 (SOD1), SOD2, and catalase (CAT), which were improved with supplementation of L. plantarum and L. helveticus [26]. By extending that study, herein, we assumed that the supplementation of these two probiotics may also affect the renal insulin signaling elements, inflammatory parameters, or glucose transporters in fructose-fed rats.…”

Protective effect of Lactobacillus plantarum ATCC8014 on acrylamide-induced oxidative damage in rats

“…In vitro, L. plantarum has an effective free radical scavenging ability and reducing ability [47,48], and can enhance the antioxidant enzyme activity of Caco-2 cells [49]. In vivo, L. plantarum not only enhances the antioxidant status of the kidney [50], liver [47,51], and heart [52], but also enhances the activity of antioxidant enzymes in the plasma of rats, and enhances the overall antioxidant level of rats [50]. Although the antioxidant activity of lactic acid bacteria has been confirmed by in vivo and in vitro tests, its specific antioxidant mechanism is not clear enough and needs further investigation.…”