Lactobacillus heilongjiangensis sp. nov., isolated from Chinese pickle

Abstract

A Gram-stain-positive bacterial strain, S4-3 T , was isolated from traditional pickle in Heilongjiang Province, China. The bacterium was characterized by a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, dnaK gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, DNA-DNA hybridization and an analysis of phenotypic features. Strain S4-3 T showed 97.9-98.7 % 16S rRNA gene sequence similarities, 84.4-94.1 % pheS gene sequence similarities and 94.4-96.9 % rpoA gene sequence similarities to the type strains of Lactobacillus nantensis, Lactobacillus mindensis, Lactobacillus crustorum, Lactobacillus futsaii, Lactobacillus farciminis and Lactobacillus kimchiensis. dnaK gene sequence similarities between S4-3 T and Lactobacillus nantensis LMG 23510 T , Lactobacillus mindensis LMG 21932 T , Lactobacillus crustorum LMG 23699 T , Lactobacillus futsaii JCM 17355 T and Lactobacillus farciminis LMG 9200 T were 95.4, 91.5, 90.4, 91.7 and 93.1 %, respectively. Based upon the data obtained in the present study, a novel species, Lactobacillus heilongjiangensis sp. nov., is proposed and the type strain is S4-3 T (5LMG 26166 T 5NCIMB 14701 T ).Pickle is rich in lactic acid bacteria (LAB). In recent years, many novel LAB species and subspecies have been isolated from pickle, such as Lactobacillus senmaizukei (Hiraga et al., et al., 2012). In the present study, a Gram-stain-positive bacterial strain, S4-3 T , was isolated from traditional pickle in Heilongjiang Province, China. The bacterium was characterized by a polyphasic approach. The strains used in this study are listed in Table 1. All strains were incubated aerobically at 30 u C on mMRS medium (pH 6.2-6.4) consisting of 0.5 % peptone, 0.5 % meat extract, 1 % tryptone, 0.5 % yeast extract, 1 % glucose, 0.1 % Tween 80, 0.2 % K 2 HPO 4 . 3H 2 O, 0.5 % sodium acetate anhydrous, 0.2 % diammonium hydrogen citrate, 0.025 % MnSO 4 . H 2 O and 0.01 % MgSO 4 . 7H 2 O.

Characterization of relative abundance of lactic acid bacteria species in French organic sourdough by cultural, qPCR and MiSeq high-throughput sequencing methods

“…heilongjiangensis had previously been found in traditional Chinese pickles (Gu et al, 2013) and was isolated here for the first time in sourdough (B23). It is a Gram-positive, catalase-negative, non-sporeforming rod and produces acid from many carbon substrates.…”

“…Isolates from group 3 were identified by rDNA 16S sequencing as belonging to L. heilongjiangensis (100%). Using rpoA and pheS genes (Gu et al, 2013;Naser et al, 2007)…”

Proposal of Lactobacillus kosoi Chiou et al. 2018 as a later heterotypic synonym of Lactobacillus micheneri McFrederick et al. 2018, elevation of Lactobacillus plantarum subsp. argentoratensis to the species level as Lactobacillus argentoratensis sp. nov., and Lactobacillus zhaodongensis sp. nov., isolated from traditional Chinese pickle and the intestinal tract of a honey bee (Apis mellifera)

“…For honey bee, the intestinal tract was collected in a sterile tube containing 0.1 ml sterile normal saline and stored in 4 °C after homogenization. A loop of pickle juice or salt solution was directly streaked on modified MRS (mMRS; pH 6.2-6.4; consisting of 0.5 % peptone, 0.5 % meat extract, 1 [23]. On the basis of the colony morphology, presumptive different strains were picked and further purified.…”

“…In the present study, two novel strains, designated 1206-1 T and F027-1-2, isolated from traditional pickle in Heilongjiang Province (PR China) and from the intestinal tract of a honey bee (Apis mellifera) in Hubei Province (PR China) respectively, were characterized by a polyphasic approach. All strains were incubated aerobically at 30 °C on mMRS agar plates [23].…”

Enterococcus xiangfangensis sp. nov., isolated from Chinese pickle

“…Reference strains are type strains of phylogenetically related species. All strains were incubated aerobically at 30 u C on mMRS (modified MRS) medium (Gu et al, 2013). DNA for gene amplification was prepared using genomic DNA extraction kits (TIANGEN).…”