Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage.

Krooneman, Janneke; Faber, Folkert; Alderkamp, Anne‐Carlijn; Elferink, S.J.H.W. Ouden; Driehuis, F.; Cleenwerck, Ilse; Swings, Jean; Gottschal, Jan C.; Vancanneyt, Marc

doi:10.1099/00207713-52-2-639

Cited by 181 publications

(149 citation statements)

References 28 publications

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“…buchneri degrades under anaerobic conditions; lactic acid in acetic acid and 1,2-propanediol; the reduction of lactic acid represents a decrease of potentially fermentable substrate for yeast (Oude Elferink et al, 2001) and acid 1,2 -propanediol as a substrate for strains of Lactobacillus diolivorans sp. nov., transformed into 1-propanol and propionic acid (Krooneman et al, 2002) . According to Moon (1983) acetic acid in combination with propionic acid shows synergistic effect capable of reducing the growth of yeasts and fungi.…”

Section: Results An Discussionmentioning

confidence: 99%

Chemical composition, fermentation, in vitro digestibility and in situ degradability of sugar cane silages with Lactobacillus, urea and agricultural byproduct

Maeda

Zeoula²,

Jobim³

et al. 2011

R. Bras. Zootec.

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-The objective of this study was to evaluate the sugarcane silage in relation to chemical composition, pH, total losses, aerobic stability, in vitro digestibility of dry matter (IVDDM) and effective degradability (ED) of bovine and buffaloes under the following treatments (on a dry matter (DM) basis): sugarcane silage control (control), with addition of 3.3% urea; with addition of Lactobacillus buchneri (inoculate); with inoculate + 3.3% urea; with inoculate + 25.0% soybean hull, and with inoculate + 25.0% cassava byproduct meal. The experiment was analyzed through a completely randomized design with three replications, and for DM ED, a double 4 × 4 Latin square with a 4 × 2 factorial arrangement was used. The sugarcane variety was the RB-855156 (precocious). The urea increased the protein level and reduced neutral and acid detergent fiber and lignin fractions. Treatments with inoculate + cassava byproduct and inoculate + soybean hull increased DM levels. Bovines presented higher IVDDM than buffaloes. pH values varied from 3.1 to 4.0. Mean total losses observed were 7.8%. Treatments with inoculate + cassava and inoculate + soybean hulls resulted in larger aerobic stability with 61 and 81 hours, respectively, in relation to silages control (24 h) and control with 3.3% urea (33 h). A higher DM ED was verified for silages with inoculate + control and inoculate + soybean hulls at 2%/h passage rates and higher neutral detergent fiber ED for the treatment of control + inoculate + soybean hulls at 2 and 5%/h. Silages treated with urea, L. buchneri or agricultural byproduct plus inoculate presented better quality pattern.

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Section: Results An Discussionmentioning

confidence: 99%

Chemical composition, fermentation, in vitro digestibility and in situ degradability of sugar cane silages with Lactobacillus, urea and agricultural byproduct

Maeda

Zeoula²,

Jobim³

et al. 2011

R. Bras. Zootec.

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“…Other strains also identified as L. buchneri (UFLA SIL 7, UFLA SIL 8 and Lalsil) did not present that effect, demonstrating that strains of the same species present different characteristics. According to Krooneman et al, (2002) bacteria of the L. diolivorans species may degrade 1,2-propanediol into propionic acid in silages. In the silages evaluated in this study, that conversion probably did not occur, as the propionic acid content was not changed in the silages.…”

Section: Discussionmentioning

confidence: 99%

New inoculants on maize silage fermentation

et al. 2014

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-The objective of this study was to evaluate the effect of bacterial inoculants at two inoculation rates on chemical and biological characteristics of maize silage. The treatments consisted of two inoculating rates (5 and 6 log cfu g −1 of forage) for each strain of lactic acid bacteria (LAB) identified as Lactobacillus buchneri, L. hilgardii, or L. plantarum. The maize was ensiled in experimental PVC silos. Samples were taken for the determination of the contents of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), water-soluble carbohydrates (WSC), organic acids and alcohols, for the evaluation of the populations of lactic acid bacteria, yeasts, filamentous fungi, and for the determination of pH values during ensilage and after 30 or 90 days of fermentation. The doses of inoculants did not promote significant differences on the evaluated characteristics. There was effect of inoculants on acetic acid, 1.2-propanediol, LAB population, filamentous fungi, and pH value. No significant influence of the treatments with inoculants was observed in the variables DM, WSC, CP, lactic acid concentrations, or ethanol. The maximum temperature, i.e., the time to achieve the maximum temperature (TMT) and aerobic stability (AS), was not influencied by treatments. However, a decrease in maximum temperature, an increase in TMT, and improvement in the AS were observed after 90 days of fermentation. These results proved the advantage of microbial inoculation. The treatments influenced LAB populations and filamentous fungi, but no effect was observed on the yeast population. The best inoculation dose is 6 cfu g −1 of forage because it provides higher reduction of filamentous fungi in maize silage, thereby decreasing the aerobic deterioration by these microorganisms.

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“…Higher concentrations of propionic and butyric acids were measured in inoculant-treated silages due to heterolactic fermentation of L. buchneri. Krooneman et al (2002) speculated that certain members of the epiphytic microfloora (L. diolivorans) are involved in the conversion of 1,2-propanediol (produced by L. buchneri) to propionic acid. The pH was higher in inoculanttreated silages due to a higher concentration of acetic acid and a lower ratio of lactic to acetic acid.…”

Section: Discussionmentioning

confidence: 99%

Ensiling corn silage with different levels of a multi-species lactic acid bacteria inoculant

2014

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Abstract.A multi-species lactic acid bacterial inoculant (Lactisil maize, LM) was applied to whole-crop corn at different maturities in laboratory silos, to evaluate its effects on biochemical characteristics and aerobic stability. The corn crop was harvested at hard dough (HD, 253.1 g/DM kg), one-third milkline (ML, 293.7 g/DM kg) and one-third milkline with a killing frost (MLF, 297.6 g/DM kg). Crops were chopped to a 2.5-cm theoretical cut length, subsampled and treated with two levels of inoculant (LB1 = 1.5 · 10 5 cfu/g forage, LB2 = 3 · 10 5 cfu/g forage) or untreated (WO). The chemical composition of MLF crops was very similar to that of ML crops. However, lower (P < 0.01) numbers of lactic acid bacteria and higher numbers of yeast were enumerated in MLF than in ML crops. Higher percentages of DM and neutral detergent fibre and higher pH, but lower (P < 0.01) concentrations of water soluble carbohydrate and crude protein were measured in ML and MLF crops than in HD crops. Application of the inoculant increased (P < 0.01) concentrations of volatile fatty acids, neutral detergent fibre and acid detergent fibre in silages. Lactic acid concentration increased (P < 0.01) in HD treatments with an increasing level of inoculant. In contrast, the highest (P < 0.01) lactic acid concentration was measured in LB1 treatment compared with WO and LB2 in ML and MLF silages. Silages prepared from ML and MLF crops had higher (P < 0.01) lactic and acetic acid concentrations but lower (P < 0.01) butyric acid concentrations than did those prepared from HD. The pH in LB1 and LB2 silages was higher (P < 0.01) than that measured in WO silages. Aerobic stability was not influenced by inoculant treatment but low-DM silages were more (P < 0.01) resistant to spoilage. Frost-killed corn crops had a good potential to produce well fermented silage. Using LM resulted in silages with slightly higher fermentation products but it failed to improve aerobic stability of silage after 120 days of ensiling. These results indicated that inoculation of corn crops with LM for a short-duration ensilage period cannot enhance aerobic stability of silages due to insufficient acetic acid production from lactic acid conversion.Additional keywords: aerobic stability, frost-killed corn, heterofermentative fermentation, homofermentative lactic acid bacteria, Lactobacillus buchneri, maturity stage.

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Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage.

Cited by 181 publications

References 28 publications

Chemical composition, fermentation, in vitro digestibility and in situ degradability of sugar cane silages with Lactobacillus, urea and agricultural byproduct

Chemical composition, fermentation, in vitro digestibility and in situ degradability of sugar cane silages with Lactobacillus, urea and agricultural byproduct

New inoculants on maize silage fermentation

Ensiling corn silage with different levels of a multi-species lactic acid bacteria inoculant

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