Lactobacillus brevis Strains from Fermented Aloe vera Survive Gastroduodenal Environment and Suppress Common Food Borne Enteropathogens

Abstract

Five novel Lactobacillus brevis strains were isolated from naturally fermented Aloe vera leaf flesh. Each strain was identified by Random Amplified Polymorphic DNA (RAPD) analysis and 16S rRNA sequence comparison. These strains were highly tolerant to acid, surviving in pH2.5 for up to 4 hours, and resistant to 5% bile salts at 37°C for 18 hours. Due to its tolerance to acid and bile salts, one strain passed through the gastric barrier and colonised the intestine after oral administration. All five strains inhibited the growth of many harmful enteropathogens without restraining most of normal commensals in the gut and hence named POAL (Probiotics Originating from Aloe Leaf) strains. Additionally, each strain exhibited discriminative resistance to a wide range of antibiotics. The L. brevis POAL strains, moreover, expressed high levels of the glutamate decarboxylase (GAD) gene which produces a beneficial neurotransmitter, γ-aminobutyric acid (GABA). These characteristics in all suggest that the novel L. brevis strains should be considered as potential food additives and resources for pharmaceutical research.

Short Chain Fatty Acids from Fermentation By Endophytic Bacteria in Aloe Vera Leaf Rind and Gel

“…Kim YW. group revealed that five novel Lactobacillus brevis strains; probiotics originating from Aloe vera leaf, were isolated from naturally fermented Aloe vera leaf flesh, and expressed high levels of the glutamate decarboxylase gene which produces a beneficial neurotransmitter, r-amino butyric acid [10] . Isolation of endophytic Ralstonia sp.…”

“…Similarly, Kim et al [10] reported that Lactobacillus brevis strains isolated from naturally fermented Aloe vera leaf flesh expressed high levels of the glutamate decarboxylase (GAD) gene which produces a beneficial neurotransmitter, γ-aminobutyric acid (GABA).…”

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“…[42] A. vera ingredients suppress common foodborne enteropathogens. [43] Aloe barbadensis Miller affects survivability, proteolysis, and ACE inhibition of potential probiotic cultures in fermented milk. [44] A. vera polysaccharides are used in blend modification of soy protein/ lauric acid to make edible films.…”

“…[171] A. vera suppresses common foodborne enteropathogens, but Lactobacillus brevis strains survive in gastroduodenal environment and. [43] A. vera gel is used as an adjuvant treatment of oral submucous fibrosis. [172] A. vera -and myrrh-based oral mucoadhesive gels are used in the management of minor recurrent aphthous stomatitis.…”

Fermented Butyrate of Aloe vera and A. arborescens by Endophytic Bacteria: Its Prophylactic Action on Brain Insulin Resistance

“…Barra et al [2] isolated Talaromyces wortmannii, as an endophyte of A. vera healthy tissue, and identified anti-biotical active biemodin, a symmetrical dimer of two emodin units. Kim et al [3] revealed that five novel Lactobacillus brevis strains; probiotics originating from A. vera leaf, were isolated from naturally fermented A.vera leaf flesh, and expressed high levels of the glutamate decarboxylase gene which produces a beneficial neurotransmitter, γ-aminobutyric acid. Akinsanya et al [4] isolated twenty-nine cultivable bacterial endophyte from A. vera surface-sterilized tissues and revealed the bioactive compounds with high DPPH scavenging properties and antimicrobial activities against bacterial pathogens.…”