Lactobacillus brantae sp. nov., isolated from faeces of Canada geese (Branta canadensis)

Abstract

Three strains of lactic acid bacteria (LAB) were isolated from the faeces of apparently healthy wild Canada geese (Branta canadensis) in 2010 by cultivating faecal LAB on Rogosa SL agar under aerobic conditions. These three isolates were found to share 99.9 % gene sequence similarity of their 16S rRNA, their 16S-23S intergenic transcribed spacer region (ITS), partial 23S rRNA, rpoB, rpoC, rpoA and pheS gene sequences. However, the three strains exhibited lower levels of sequence similarity of these genetic targets to all known LAB, and the phylogenetically closest species to the geese strains were Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus and Lactobacillus saniviri. In comparison to L. casei ATCC 393 T , L. paracasei ATCC 25302 T , L. rhamnosus ATCC 7469 T and L. saniviri DSM 24301 T , the novel isolates reacted uniquely in tests for cellobiose, galactose, mannitol, citric acid, aesculin and dextrin, and gave negative results in tests for L-proline arylamidase and L-pyrrolydonyl-arylamidase, and in the Voges-Proskauer test. Biochemical tests for cellobiose, aesculin, galactose, gentiobiose, mannitol, melezitose, ribose, salicin, sucrose, trehalose, raffinose, turanose, amygdalin and arbutin could be used for differentiation between L. saniviri and the novel strains. On the basis of phenotypic and genotypic characteristics, and phylogenetic data, the three isolates represent a novel species of the genus Lactobacillus, for which the name Lactobacillus brantae sp. nov. is proposed. The type strain is SL1108 T (5ATCC BAA-2142 T 5LMG 26001 T 5DSM 23927 T ) and two additional strains are SL1170 and SL60106.

Evolution of Bacterial Communities, Physicochemical Changes and Sensorial Attributes of Natural Whole and Cracked Picual Table Olives During Spontaneous and Inoculated Fermentation

“…However, the contribution of LAB in the production of this acid could also be taken into consideration. Potentially heterofermentative LAB, such as L. manihotivorans (Salvetti et al, 2012) and L. brantae (Volokhov et al, 2012), may generate acetic acid from fermentable material under particular conditions of environmental stress as well as from the metabolism of citric acid (Laëtitia et al, 2014). Finally, the presence of succinic acid only in inoculated treatments, could be related to the microbial conversion of citric acid to succinic, via a potential shift from homo-to heterofermentative metabolism of the starter culture (Papadelli et al, 2015).…”

A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae

“…Original characteristics of L. brantae strains are provided by [159]. The genome size of the type strain is 1.93 Mbp.…”

Streptococcus vicugnae sp. nov., isolated from faeces of alpacas (Vicugna pacos) and cattle (Bos taurus), Streptococcus zalophi sp. nov., and Streptococcus pacificus sp. nov., isolated from respiratory tract of California sea lions (Zalophus californianus)

“…We conducted a previous study from 2007 to 2012 to collect specimens from different animal sources for the isolation and phylogenetic characterization of Lactobacillus and Mycoplasma spp. [21, 22]. During this previous study, we also isolated Streptococcus sp.…”