Lactobacillus acidophilus Strain Suppresses the Transcription of Proinflammatory-Related Factors in Human HT-29 Cells

Abstract

Previous studies have shown that lactic acid bacteria can inhibit inflammatory responses, but the mechanisms are very little known. In this study, transaction and expression of three proinflammatory factors, iNOS, PTGS-2, and IL8, which are closely related to the inflammatory response, were investigated by luciferase reporter assay and RTPCR in HT-29 cells treated by Lactobacillus acidophilus. The results showed that the live L. acidophilus sharply down-regulated the transcription of these three genes. Because there was a NF-κB binding site located at -265 bp, -225 bp, and -95 bp upstream of the iNOS, PTGS-2, and IL8 promoters, respectively, we further addressed the effects of NF-κB on transaction of the three promoters by cotransfection. As was expected, NF-κBs remarkably upregulated the activity of the reporter gene and, no effect of NF-κBs on IL-8 promoter transaction was found after NF-κB binding site mutation of the IL8 promoter in HT-29 cells. In conclusion, the live L. acidophilus decreased the transcriptional activity of NF-κB and, in turn, inhibited the transaction of NF-κB on the three proinflammatory factors mentioned above.

Probiotic and anti-inflammatory attributes of an isolate Lactobacillus helveticus NS8 from Mongolian fermented koumiss

“…To investigate the surface properties of NS8, the isolate as well as food-originated probiotic strains L. acidophilus 1.2 [ 18 ] and L. plantarum TH1 [ 19 ] (provided by Prof. XG Luo, Tianjin University of Science and Technology) were subjected to an array of tests. As shown in Table 1 , the adhesion ratio of NS8 isolate with Caco-2 cell culture was estimated to be 18.03 %, which was significantly higher than the other probiotic strains with values of 4.58 % of L. acidophilus 1.2 and 3.34 % of L. plantarum TH1 respectively.…”

Lactobacillus acidophilus suppresses intestinal inflammation by inhibiting endoplasmic reticulum stress

“…We showed that both LA1 and SL4 ( P. pentosaceus ) consistently suppressed NF‐κB activation in IECs, NO production by macrophages, and colonic iNOs expression. Some components of LA1 such as cell wall‐associated polysaccharides, proteins, and lipoteichoic acids could directly inhibit NF‐κB activation in human HT‐29 cells . LA1 can also inhibit the NF‐κB activation by secreted metabolites, cell wall components, bacterial DNA, and bacteria‐secreted soluble unknown factors that can be mediated by upregulating the negative regulators of TLR signaling or downregulating TLR .…”

Effects of Taro (Colocasia esculenta) Water-Soluble Non-Starch Polysaccharide, Lactobacillus acidophilus, Bifidobacterium breve, Bifidobacterium infantis, and Their Synbiotic Mixtures on Pro-Inflammatory Cytokine Interleukin-8 Production

“…Regarding the cytotoxicity of Tc-WS-NSP, our previous study [ 40 ] demonstrated the lack of or minimal cytotoxicity of both the undigested or digested Tc-WS-NSPs extracted using the CE and ICE methods in HT-29 cells in a concentration range of 0.5 to 7.5 mg/mL. Regarding the cytotoxicity of the probiotics, a number of studies on L. acidophilus , B. breve , and B. infantis [ 54 , 55 , 56 , 57 ] have shown that these probiotics have no cytotoxic effects on cells including HT-29 cell culture. This has been established based on the safety profile of various clinical trials using these probiotics [ 58 , 59 , 60 , 61 ].…”