Lactobacilli: Fact and Fiction

Conway, Patricia L.

doi:10.1007/978-1-349-10723-0_16

Cited by 44 publications

(21 citation statements)

References 75 publications

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“…The lack of effects of the diets on bacterial populations (Table 4) needs to be interpreted with caution due to the importance of bacteria attached to the mucosa of the stomach and small intestine, as reviewed by Savage (1977) and Conway (1989). The results of this study agree with those of Risley et al (1992).…”

Section: Resultssupporting

confidence: 82%

“…The results of this study agree with those of Risley et al (1992). Kirchgessner et al (1992) (Muralidhara et al 1977) and decreased incidence of diarrhea (Luckey 1984). However, when attempts are made to confirm that ktctobacillus species improve the health of the digestive tract nonsignificant results are often reported (Conway 1989 This may, in part, explain why there were minor effects of the experimental treatments. Therefore, these results may not reflect those obtained within days of weaning.…”

Section: Resultssupporting

confidence: 78%

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The effect of formic acid and buffering capacity on the ileal digestibilities of amino acids and bacterial populations and metabolites in the small intestine of weanling pigs fed semipurified fish meal diets

Gabert¹,

Sauer²,

Schmitz³

et al. 1995

Can. J. Anim. Sci.

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R. 1995. The effect of formic acid and buffering capacity on the ileal digestibilities of amino acids and bacterial populations and metabolites in the small intestine of weanling pigs fld semipurified fish meal diets. Can. J. Anim. Sci. 75: 615423. An experiment was carried out to examine the effects of supplementa:tion of formic acid to semipurified diets containing fish meal for weanling pigs with low and high buffering capacity on nutrient digestion and bacterial populations and metabolites in the small intestine. Twelve barrows, weaned at 2l d, were fitted with a simple T-cannula at the distil ileum. The BW of the pigs at weaning and at the conclusion of the experiment were.7.8 and 13.8 kg, respectively. The pigs were fed four corn starch-based fish meal diets according to a two-period change-over design' The diets were fbrmulated to contain lSVo CP. For two of the diets, referred to as NCaP, fish meal was the calcium and phosphorus source. For the remaining two diets, referred to as HCaP, the levels of calcium and phosphorus were doubled (compared with the NCap diets) by the addition of calcium carbonate and dicalcium phosphate. The diets were supplemented with (+NCaP and +HCaP) or wit|out (-NCaP and -HCaP) l7o (wt|wt) formic acid. The buffering capacity of the HCaP diets increased (P < 0.05) when the levels of calcium and phosphorus were doubled. The pH of the diets were; 6.25, 4.46,6.13 and 4.59 for the -NCaP' +NCaP, -HCaP and +HCaP diets, respectively. Supplementation of formic acid to diets with low and high buffering capacity did not affect (P > 0.05) the apparent ileal digestibilities of AA. The pH, ammonia and VFA concenfiations, bacterial populations in ileal digesta and the inciderrce of diarrhea were also not affected (P > 0.05). Therefore, the supplementation of l7o (wt/wt) formic acid to corn starch-based fish meal diets with low and high buffering capacity for weanling pigs (7.8-13.8 kg) does not affect nutrient digestibilities and bacterial populations in the small intestine.

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Section: Resultssupporting

confidence: 82%

Section: Resultssupporting

confidence: 78%

The effect of formic acid and buffering capacity on the ileal digestibilities of amino acids and bacterial populations and metabolites in the small intestine of weanling pigs fed semipurified fish meal diets

Gabert¹,

Sauer²,

Schmitz³

et al. 1995

Can. J. Anim. Sci.

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“…Some lactobacilli adhere to freshly isolated uro-epithelial cells (Chan et al, 1984(Chan et al, ,1985Reid & Sobel, 1987) and living adhering lactobacilli or cellwall fragments possess inhibiting properties against adhesion of uro-pathogenic Escherichia coZi (Chan et al, 1985;. Contradictory results have been obtained for the gastrointestinal tract (reviewed by Conway, 1988). Recently, using Caco-2 cells as an intestinal cell model, we have reported an inhibition of diarrheagenic Escherichia coli adhesion by the human L. acidophilus strain LB (Chauviere et al, 1992).…”

Section: Introductionmentioning

confidence: 73%

“…Recently, using Caco-2 cells as an intestinal cell model, we have reported an inhibition of diarrheagenic Escherichia coli adhesion by the human L. acidophilus strain LB (Chauviere et al, 1992). However, little is currently known about the mechanism of adhesion of lactobacilli to human intestinal cells (Conway, 1988;Reid et al, 1990). Kleeman & Klaenhammer (1 982) screened 27 human Lactobacillus isolates and reported that four L. acidophilus strains, BG2F04, MSOl, MS02 and MS04 adhered specifically, in a calcium-independent manner, to human fetal intestinal epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells

Chauvière

Coconnier

Kernéis

et al. 1992

Journal of General Microbiology

219

140

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Twenty-five strains of lactobacilli were tested for their ability to adhere to human enterocyte-like Caco-2 cells in culture. Seven Lactobacillus strains adhered well to the Caco-2 cells, of which three possessed calcium-independent adhesion properties. A high level of calcium-independent adhesion was observed with the human stool isolate Lactobacillus acidophilus strain LB. Scanning electron microscopy revealed that this strain adhered to the apical brush border of the cells. Adhesion increased in parallel with the morphological and functional differentiation of the Caco-2 cells. Two Lactobacillus components were involved in this adhesion. One was protease-resistant and bacterial-surface-associated; the other was heat-stable, extracellular and protease-sensitive.

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“…Adhesion to the intestinal mucosa is probably a major determinant of colonization by the exogenous strains (Conway, 1988). Compared with the great deal of knowledge concerning pathogens, little is known about the adhesion capacity and mechanisms of organisms used as probiotics.…”

mentioning

confidence: 99%