Manipulation of the human gut microflora

Rambaud, Jean–Claude; Bouhnik, Yoram; Marteau, Philippe; Pochart, Philippe

doi:10.1079/pns19930071

Cited by 29 publications

(23 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1 Then, we studied the relationship between Con A agglutination by lactobacilli and their adhesion to vaginal EC. Estimation of coefficient of paired correlation between agglutination titer (Table 1) and mean level of adhesion to target cells showed r=0.15, in other words, no correlation between these parameters, which confirms our previous conclusion on the presence of other adhesin types (in addition to Con A-reactive lectin-dependent adhesin) providing tropism in fixation on various target cells [5,7]. Our next step was to clear out whether GPA selectively reacting with Con A can be detected not only on lactobacillus surface, but in culture medium as well.…”

Section: Resultssupporting

confidence: 87%

Surface adhesins of lactobacilli loosely connected to the cell wall and eliminated into the environment during culturing in liquid nutrient media

et al. 2006

View full text Add to dashboard Cite

Six lactobacillus species and 4 clones of one of them were studied in order to clear out the ratio between the adhesion capacities of concanavalin A-reactive glycoprotein adhesins on the surface of the bacterial cell and glycoprotein adhesins released into the broth during culturing in liquid nutrient media. The adhesive activity of cultures is largely determined by the strain rather than species appurtenance. Elimination of glycoprotein adhesins from the bacterial cell and their antagonistic activity towards Candida albicans were demonstrated in specific interactions of glycoprotein adhesins with immune serum and concanavalin A.

show abstract

Section: Resultssupporting

confidence: 87%

Surface adhesins of lactobacilli loosely connected to the cell wall and eliminated into the environment during culturing in liquid nutrient media

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Potential anticarcinogenic properties of bacterial species also vary greatly between the strains and only certain strains of lactic acid bacteria may affect the outcome of colorectal cancers (Lidbeck et al, 1992;Pool-Zobel et al, 1996). Furthermore, lactic acid bacteria commonly used in commercially manufactured fermented milk products survive poorly in the highly acidic gastric milieu (Rambaud et al, 1993;Pedrosa et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Prospective study on milk products, calcium and cancers of the colon and rectum

et al. 2001

View full text Add to dashboard Cite

Objective: To study the relationship between consumption of milk and milk products, calcium, lactose and vitamin D and occurrence of colorectal cancers. Design: Prospective cohort study. Subjects: A total of 9959 men and women aged 15 y or older without history of cancer at baseline. During a 24 y follow-up, 72 new cancers of the large bowel (38 in the colon and 34 in the rectum) were detected. Results: Consumption of milk and total milk products was suggested to be inversely related to colon cancer incidence, whereas no similar association was seen for rectal cancer. The relative risk between the highest and lowest quartiles of intake adjusted for potential confounding factors was 0.46 (95% confidence interval 0.14 -1.46, P for trend 0.09) for milk and 0.37 (95% CI ¼ 0.12 -1.39, P for trend 0.06) for total milk products. Lactose intake showed a similar inverse relationship with colon cancer: the relative risk was 0.31 (95% CI ¼ 0.08 -1.15, P for trend 0.03). Intake of vitamin D or total dietary calcium was not significantly related to colorectal cancer risk, whereas calcium provided by fermented milk products was associated with increased colorectal cancer incidence; in the highest quartile the multivariate adjusted relative risk for colorectal cancer was 2.07 (95% CI ¼ 1.00 -4.28). Conclusions: Our results indicate that individuals showing high consumption of milk have a potentially reduced risk of colon cancer; however, the association does not appear to be due to intake of calcium, vitamin D, or to specific effects of fermented milk. Sponsorship: This study was supported by a grant from the Swedish Cancer Foundation.

show abstract

“…Unfortunately, most studies on probiotic actions have ignored this basic requirement and, as a result, data on the tolerance of potentially probiotic lactobacilli and bifidobacteria to small intestinal secretions, other than bile, are not yet available Mikelsaar and Mänder 1994). Moreover, a probiotic micro-organism cannot affect its environment unless its population reaches a certain minimum level which has not yet been exactly determined, but is probably between 10 6 and 10 8 cfu g −1 of intestinal contents Rambaud et al 1993).…”

Section: Manipulation Of the Human Gastrointestinal Microbiota Is Curmentioning

confidence: 99%

Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract

et al. 1998

View full text Add to dashboard Cite

An in vitro methodology which mimics in vivo human upper gastrointestinal transit was developed. The transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species was determined by exposing washed cell suspensions at 37°C to a simulated gastric juice (pH 2·0), containing pepsin (0·3% w/v) and sodium chloride (0·5% w/v), and a simulated small intestinal juice (pH 8·0), containing pancreatin USP (1 g l−1) and sodium chloride (5 g l−1), and monitoring changes in total viable count periodically. The methodology was also employed to determine the effect of adding milk proteins (1 g l−1), hog gastric mucin (1 g l−1) and soyabean trypsin‐chymotrypsin inhibitor [SBTCI] (1 g l−1) on transit tolerance. The majority (14 of 15) of isolates lost >90% viability during simulated gastric transit. Only one isolate, Lactobacillus fermentum KLD, was considered intrinsically resistant. The addition of milk proteins, singly and in combination, generally improved gastric transit tolerance. In this regard, two isolates, Lact. casei 212.3 and Bifidobacterium infantis 25962, exhibited 100% gastric transit tolerance in the presence of milk proteins. In general, the addition of hog gastric mucin did not influence simulated gastric transit tolerance of lactobacilli but tended to increase that of bifidobacteria. However, it increased that of Lact. casei 242 and Lact. salivarius 43338 but diminished that of B. bifidum 2715 and B. animalis Bo. Selected bile salts‐resistant isolates were intrinsically tolerant to simulated small intestinal transit. Only Lact. casei F19 and B. adolescentis 15703T showed significant reduction in viability after 240 min. In general, the addition of milk proteins and SBTCI did not affect simulated small intestinal transit tolerance. However, they significantly improved the intrinsic resistance of Lact. casei F19 but diminished that of B. breve 15700T. It is concluded that, whereas the majority of bile salts‐resistant lactobacilli and bifidobacteria may be intrinsically sensitive to gastric transit, they are intrinsically resistant to small intestinal transit. In addition, it is postulated that milk proteins and mucin may function as both buffering agents and inhibitors of digestive protease activity in vivo, thereby protecting ingested bacterial strains during upper gastrointestinal transit.

show abstract

Manipulation of the human gut microflora

Cited by 29 publications

References 32 publications

Surface adhesins of lactobacilli loosely connected to the cell wall and eliminated into the environment during culturing in liquid nutrient media

Surface adhesins of lactobacilli loosely connected to the cell wall and eliminated into the environment during culturing in liquid nutrient media

Prospective study on milk products, calcium and cancers of the colon and rectum

Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract

Contact Info

Product

Resources

About