Lactic dehydrogenase isoenzyme in cerebrospinal fluid of children with febrile convulsions

Abstract

dehydrogenase isoenzyme in cerebrospinal fluid of children with febrile convulsions. Acta Paediatr 2003; 92: 186-189. Stockholm. ISSN 0803-5253Aim: To study the lactic dehydrogenase isoenzyme values in children with simple and complex febrile convulsions. Methods: Cerebrospinal fluid samples were collected from 115 children, 57 with simple febrile convulsions, 27 with complex febrile convulsions and 31 with no neurological or intracranial pathology (controls). Lactic dehydrogenase activity and isoenzyme levels were measured on a Hitachi analyser. Results: Mean total lactic dehydrogenase activity was similar in the three groups. In the control group, lactic dehydrogenase-1 was the main fraction, followed by lactic dehydrogenase-2 and lactic dehydrogenase-3; only small percentages of lactic dehydrogenase-4 and lactic dehydrogenase-5 were detected. In the febrile convulsion group, the lactic dehydrogenase-1 fraction percentage was lower and lactic dehydrogenase-2, lactic dehydrogenase-3 percentages were higher than those in the control group; and the differences were statistically significant between the control and study groups (p < 0.01). Values of lactic dehydrogenase-4 and lactic dehydrogenase-5 were similar in all three groups.Conclusion: This is the first report on the lactic dehydrogenase isoenzyme pattern in the cerebrospinal fluid of patients with simple and complex febrile convulsions. The important finding that focal and general febrile convulsions are not associated with cell damage and changes in aerobic and anaerobic metabolism as lactic dehydrogenase remained unchanged. Analysis of cerebrospinal fluid lactic dehydrogenase isoenzyme levels can assist clinicians in differentiating febrile convulsions from clinical situations that might mimic them.

The Synthesis and Evaluation of Novel Lactate Dehydrogenase Nanobioconjugates

“…LDH detection is helpful in evaluating cytotoxic potential of compounds in food, cosmetic, pharmaceutics as well as for their environmental and medical applications, and to detect cell death in bioreactors [18][19][20][21][22]. Level of LDH increases when brain tissue is damaged in hydrocephalus, brain tumors and epilepsy [23,24]. It, therefore, serves as a general indicator to assess cytotoxicity resulting from cancer metathesis and to enumerate cell-mediated toxicity induced by cytotoxic T cells, lymphokine-activated killer cells, natural killer cells, monocytes and to identify cytolysis incurring agents [25].…”