Lactic-acid bacteria increase the survival of marine shrimp, Litopenaeus vannamei, after infection with Vibrio harveyi

Vieira, Felipe do Nascimento; Pedrotti, Fabiola Santiago; Neto, Celso Carlos Buglione; Mouriño, José Luiz Pedreira; Beltrame, Elpídio; Martins, Maurício Laterça; Ramírez, Cristina; Arana, Luis Alejandro Vinatea

doi:10.1590/s1679-87592007000400002

Cited by 57 publications

(53 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We used the probiotic strain L. plantarum (accession #CPQBA 007 07 DRM01), which was isolated from an adult shrimp (L. vannamei) (Vieira et al, 2007).…”

Section: Methodsmentioning

confidence: 99%

“…However, most studies on probiotics and marine crustaceans have been performed on a small laboratory scale and under controlled laboratory conditions (Vieira et al, 2007(Vieira et al, , 2008(Vieira et al, , 2010Pham et al, 2014;Ferreira et al, 2015). Because laboratory conditions do not always match the conditions on commercial farms, there is a need for studies on the use of probiotics on commercial farms.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Use of probiotic-supplemented diet on a Pacific white shrimp farm

et al. 2016

Self Cite

View full text Add to dashboard Cite

-The objective of this study was to evaluate the use of a diet supplemented with Lactobacillus plantarum on performance, immunological parameters, and bacterial microbiota of the digestive tract of white leg shrimp (Litopenaeus vannamei). We used six earthen ponds (1.2 ha) stocked with 14 shrimp m −2 (3 g mean weight). Three ponds received a diet supplemented with L. plantarum and three were maintained as control. The survival and feed efficiency of shrimp fed the supplemented diet were greater than that observed in shrimp fed the control diet, with survival values of 83.02±6.12% and 74.65±9.07% and feed efficiencies of 117.97±4.45% and 104.46±7.30%, respectively. However, we observed no differences in weight gain. The intestines of shrimp fed probiotics had lower counts of Vibrio spp. and higher counts of lactic acid bacteria, compared with those of control shrimp. Diets supplemented with L. plantarum alter the intestinal bacterial microbiota of shrimp, resulting in increased survival and feed efficiency.

show abstract

“…We used the probiotic strain L. plantarum (accession #CPQBA 007 07 DRM01), which was isolated from an adult shrimp (L. vannamei) (Vieira et al, 2007).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of probiotic-supplemented diet on a Pacific white shrimp farm

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…The lactic acid bacterium, Lactobacillus plantarum, was isolated from 30±3g L. vannamei broodstock shrimp reared at the same laboratory (Vieira et al, 2007). This bacterium was maintained in the CPQBA collection of microorganisms, Universidade Estadual de Campinas (UNICAMP), Brazil, under access number 007 07 DRM01.…”

Section: Methodsmentioning

confidence: 99%

Effect of probiotic supplemented diet on marine shrimp survival after challenge with Vibrio harveyi

Vieira

Buglione

Mouriño

et al. 2010

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

The effect of a Lactobacillus plantarum-supplemented diet on shrimp growth, digestive tract bacterial microbiota, survival, and some hemato-immunological parameters after an experimental challenge with Vibrio harveyi was studied. No difference (P>0.05) was observed in shrimp survival (80±6%) and final weight (6.63±0.56g) after 60 days feeding trial. Total bacteria count and Vibrio spp. count in the digestive tract were not diferent (P>0.05) until day 40, but they were lower (P<0.05) in the probiotic-supplemented group on day 60. Total lactic bacteria in the shrimp digestive tract was higher after day 20 in the probiotic-supplemented group. Ten hours after V. harveyi challenge, survival of the probiotic-supplemented group (65.7±2.9%) was higher (P>0.05) than the control group (39.9±4.4%). Bacterial counts in hemolymph and hepatopancreas was lower (P<0.05) in the probiotic-supplemented group than in the control group after V. harveyi challenge. Total hemocyte count and serum agglutination activity were higher (P>0.05) in the probiotic-supplemented group after challenge with V. harveyi. Probiotic-supplemented diet modifies shrimp digestive tract bacterial microbiota, increasing resistance to V. harveyi infection. Keywords: Litopenaeus vannamei, Lactobacillus plantarum, intestinal microbiota, immunostimulation RESUMO Avaliou-se o efeito de uma dieta suplementada com Lactobacillus plantarum na engorda de

show abstract

“…The lactic acid bacterium L. plantarum was isolated from 30±3 g L. vannamei broodstock shrimps, reared at the same laboratory (Vieira et al, 2007). This bacteria was identified based on a series of physiological and biochemical parameters (Kit API 50CH).…”

Section: Methodsmentioning

confidence: 99%

“…Nevertheless, the period in which bacterial microbiota of shrimp digestive tract remains modified, and the interval in which the immune system is stimulated after probiotic administration is still unknown. Lactobacillus plantarum has already been reported to enhance Litopenaeus vannamei larvae survival and resistance to Vibrio harveyi challenge (Vieira et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant

Vieira

Neto

Mouriño

et al. 2008

Pesq. agropec. bras.

Self Cite

View full text Add to dashboard Cite

-The objective of this work was to assess the time-related action of probiotic Lactobacillus plantarum in the bacterial microbiota of the digestive tract of Litopenaeus vannamei, and the relation of total haemocyte count and serum phenol oxidase activity of shrimp challenged with Vibrio harveyi. Shrimps were fed with a probiotic-supplemented diet, for eight days, then shifted to a commercial diet. Shrimps fed only with the commercial diet served as control. Evaluations were made on the 8 th day of experiment and repeated two, four, six and eight days later. Total lactic bacteria in the digestive tract was higher until the 4 th day of evaluation in the probioticsupplemented group. Vibrio spp. counts were higher in the control at days zero and two. Until the 4 th day of evaluation, the total haemocyte counts in shrimps after challenge with V. harveyi were higher in probioticsupplemented group than in control group. Significant difference was not observed in phenol oxidase activity. On the 6 th day after shifting from supplemented to control diet, all parameters were equal in both groups, suggesting that the time-related action of L. plantarum in shrimp is short.Index terms: Litopenaeus vannamei, Vibrio harveyi, total haemocyte count, lactic bacteria, phenol oxidase, probiotic. Tempo de atuação de Lactobacillus plantarum na microbiota bacteriana intestinal de camarão e sua ação como imunoestimulanteResumo -O objetivo deste trabalho foi avaliar o tempo de atuação de Lactobacillus plantarum, na microbiota bacteriana intestinal de Litopenaeus vannamei, e sua relação com a contagem total de hemócitos e a atividade da fenoloxidase após a exposição a Vibrio harveyi. Os camarões foram alimentados com dieta suplementada com probióticos por oito dias e, depois, substituída por dieta comercial. Os camarões alimentados com dieta comercial constituíram o controle. As avaliações foram realizadas no oitavo dia de experimento e repetidas dois, quatro, seis e oito dias depois. A contagem total de bactérias lácticas no intestino foi maior até o quarto dia de avaliação, no grupo alimentado com probióticos. A contagem de Vibrio spp. foi superior no grupo controle nos dias zero e dois. Até o quarto dia, a contagem total de hemócitos dos camarões após a inoculação de V. harveyi foi superior no grupo alimentado com probiótico em relação ao grupo controle. Não foi observada diferença na atividade da fenoloxidase. No sexto dia, após a substituição da dieta suplementada com probióticos por dieta controle, todos os parâmetros avaliados foram iguais nos dois grupos, o que indica que o tempo de atuação de L. plantarum em camarões é curto.Termos para indexação: Litopenaeus vannamei, Vibrio harveyi, contagem total de hemócitos, bactérias lácticas, fenoloxidase, probiótico.

show abstract

Lactic-acid bacteria increase the survival of marine shrimp, Litopenaeus vannamei, after infection with Vibrio harveyi

Cited by 57 publications

References 15 publications

Use of probiotic-supplemented diet on a Pacific white shrimp farm

Use of probiotic-supplemented diet on a Pacific white shrimp farm

Effect of probiotic supplemented diet on marine shrimp survival after challenge with Vibrio harveyi

Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant

Contact Info

Product

Resources

About