Lactation is a natural model of hippocampus neuroprotection against excitotoxicity

Cabrera, Verónica Ortega; Cantu, Daniel A.; Ramos, Eugénia; Vanoye-Carlo, América; Cerbón, Marco; Morales, Teresa

doi:10.1016/j.neulet.2009.06.017

Cited by 36 publications

(22 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The neuroprotective action of PRL observed in the Syto-13/PI-staining and in the LDH assay presented in this study are in accordance with previous reports by our group, in which we demonstrate that PRL stimulates hippocampal neuronal survival, both in lactating rats [13,14] and in ovariectomized PRL-treated animals [16]. Interestingly, the LDH assay showed a small participation of necrotic cell death during Glu excitotoxicity as compared with the PRL effect reducing the apoptotic component of the cell death induced by Glu.…”

Section: Discussionsupporting

confidence: 94%

“…We have previously reported, that PRL provides neuroprotection against excitotoxicity in vivo [13,14,16], and that it mediates (via its receptor) neuroprotection against Glu-induced excitotoxicity in rat primary cultures of hippocampal neurons [15]. In addition, other reports have also demonstrated that PRL may protect against cell damage by causing changes in neurotransmission [34,35], as well as in the regulation of neurotrophic factors and astrocyte proliferation [36].…”

Section: Discussionmentioning

confidence: 99%

“…Among the latter effects are the stimulation of neurogenesis in the olfactory bulb [9], glia activation [10], remyelination of oligodendrocytes [11], and proliferation of precursor cells in the adult mouse hippocampus [12]. Both in vivo and in vitro studies by our group, have the increased evidence supporting that PRL affords neuroprotection against excitotoxicity induced by kainic acid and Glu [13–16]. …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prolactin-induced neuroprotection against glutamate excitotoxicity is mediated by the reduction of [Ca2+]i overload and NF-κB activation

et al. 2017

Self Cite

View full text Add to dashboard Cite

Prolactin (PRL) is a peptidic hormone that displays pleiotropic functions in the organism including different actions in the brain. PRL exerts a neuroprotective effect against excitotoxicity produced by glutamate (Glu) or kainic acid in both in vitro and in vivo models. It is well known that Glu excitotoxicity causes cell death through apoptotic or necrotic pathways due to intracellular calcium ([Ca2+] i) overload. Therefore, the aim of the present study was to assess the molecular mechanisms by which PRL maintains cellular viability of primary cultures of rat hippocampal neurons exposed to Glu excitotoxicity. We determined cell viability by monitoring mitochondrial activity and using fluorescent markers for viable and dead cells. The intracellular calcium level was determined by a fluorometric assay and proteins involved in the apoptotic pathway were determined by immunoblot. Our results demonstrated that PRL afforded neuroprotection against Glu excitotoxicity, as evidenced by a decrease in propidium iodide staining and by the decrease of the LDH activity. In addition, the MTT assay shows that PRL maintains normal mitochondrial activity even in neurons exposed to Glu. Furthermore, the Glu-induced intracellular [Ca2+]i overload was attenuated by PRL. These data correlate with the reduction found in the level of active caspase-3 and the pro-apoptotic ratio (Bax/Bcl-2). Concomitantly, PRL elicited the nuclear translocation of the transcriptional factor NF-κB, which was detected by immunofluorescence and confocal microscopy. To our knowledge, this is the first report demonstrating that PRL prevents Glu excitotoxicity by a mechanism involving the restoration of the intracellular calcium homeostasis and mitochondrial activity, as well as an anti-apoptotic action possibly mediated by the activity of NF-κB. Overall, the current results suggest that PRL could be of potential therapeutic advantage in the treatment of neurodegenerative diseases.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prolactin-induced neuroprotection against glutamate excitotoxicity is mediated by the reduction of [Ca2+]i overload and NF-κB activation

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…This process is associated with the capacity to repair white matter damage in the maternal CNS by increasing myelin basic protein expression (Gregg, 2009). During lactation, a physiological hyperprolactinemic state, there is a reduced sensitivity to kainic acid-induced cell damage in the dorsal hippocampus of the dam, showing that lactation is a natural model of neuroprotection (Vanoye-Carlo et al, 2008; Cabrera et al, 2009) in which PRL can participate. PRL systemic administration has been reported to protect the dorsal hippocampus of female rats against excitotoxicity induced by kainic acid administration, blocking cell loss and neurodegeneration, and diminishing the progression of kainate-generated behavioral manifestation of seizures (Tejadilla et al, 2010).…”

Section: Hpa Axis Hormones Progesterone and Prolactin In The Regulamentioning

confidence: 99%

Hormonal influences on neuroimmune responses in the CNS of females

Monasterio

Vergara

Morales

2014

Front. Integr. Neurosci.

Self Cite

View full text Add to dashboard Cite

Particular reproductive stages such as lactation impose demands on the female. To cope with these demands, her physiology goes through numerous adaptations, for example, attenuation of immune and stress responses. Hormonal fluctuation during lactation exerts a strong influence, inducing neuroplasticity in the hypothalamus and extrahypothalamic regions, and diminishing the stress and inflammatory responses. Thus, hormones confer decreased vulnerability to the female brain. This mini-review focuses on the adaptations of the immune and stress response during maternity, and on the neuroprotective actions of progesterone and prolactin and their effects on inflammation. The importance of pregnancy and lactation as experimental models to study immune responses and disease is also highlighted.

show abstract

“…Lactation is a complex physiological condition that often requires a larger energy supply than pregnancy and induces a series of adaptations in the physiology of the female, including an increase of maternal brain plasticity [4]. After lactation, the gland goes through a process of apoptotic cell death and remodeling known as involution and often described as a two-step process because of its reversibility [4], [5].…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of Serotonin, Tryptophan Hydroxylase and Monoamine Oxidase A in the Mammary Gland of the Myotis velifer Bat

et al. 2013

View full text Add to dashboard Cite

The mammary gland has long drawn the attention of the scientific community due to the limited knowledge of some fundamental aspects involved in the control of its function. Myotis velifer, a microchiropteran species, provides an interesting model to study some of the regulatory factors involved in the control of the mammary gland cycle. Having an asynchronous, monoestrous reproductive pattern, female M. velifer bats undergo drastic morphological changes of the breast during the reproductive cycle. Current research on non-chiropteran mammals indicates that serotonin (5-HT) plays a major role in the intraluminal volume homeostasis of the mammary gland during lactation; however, an analysis of both the expression and localization of the main components of the serotonergic system in the bat mammary gland is lacking. Thus, the objectives of the present study were: to describe the gross and histological anatomy of the mammary gland of M. velifer to establish the lactation period for this species; to analyze the distribution and expression of the main serotonergic components in the mammary tissues of these bats under the physiological conditions of lactation, involution and the resting phase; and to provide information on the involvement of 5-HT in the regulation of the physiological function of this organ. To assess the expression and localization of serotonergic components, multiple immunofluorescence, Western blot and HPLC methods were used. 5-HT and the enzyme that catalyzes its synthesis (TPH) were located in both myoepithelial and luminal epithelial cells, while the enzyme responsible for the catabolism of this neurohormone (MAO A) was found in luminal epithelial cells as well as in secreted products. We also found an increased expression of serotonergic components during lactation, indicating that elements of the serotonergic system may play an important role in lactation in this species of bat in a way similar to that of other mammal species.

show abstract

Lactation is a natural model of hippocampus neuroprotection against excitotoxicity

Cited by 36 publications

References 28 publications

Prolactin-induced neuroprotection against glutamate excitotoxicity is mediated by the reduction of [Ca2+]i overload and NF-κB activation

Prolactin-induced neuroprotection against glutamate excitotoxicity is mediated by the reduction of [Ca2+]i overload and NF-κB activation

Hormonal influences on neuroimmune responses in the CNS of females

Differential Expression of Serotonin, Tryptophan Hydroxylase and Monoamine Oxidase A in the Mammary Gland of the Myotis velifer Bat

Contact Info

Product

Resources

About