Lactation driven dynamics of adiponectin supply from different fat depots to circulation in cows

Singh, Shiva P.; Häußler, Susanne; Heinz, Johanna F.L.; Akter, Salma; Saremi, B.; Müller, Ute; Rehage, J.; Dänicke, Sven; Mielenz, M.; Sauerwein, H.

doi:10.1016/j.domaniend.2013.12.001

Cited by 21 publications

(20 citation statements)

References 62 publications

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“…Similar findings were also observed in previous reports (18,25). In addition, Singh et al (25) reported that not only serum adiponectin levels but also levels of adiponectin in fat tissue decreased around parturition, concluding that the drop in adiponectin level could be related to parturition-related hormonal changes or increased secretion of adiponectin into colostrum. Increased lipolysis and decreased lypogenesis around parturition causes elevation of blood NEFA levels (17).…”

Section: Discussionsupporting

confidence: 88%

Changes in circulating adiponectin and tumour necrosis factor-α and their relationship with insulin resistance in periparturient dairy cows

Mecitoğlu

Şentürk

Akgül

et al. 2016

Journal of Veterinary Research

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Introduction: The aim of the study was to investigate changes in the serum levels of adiponectin and TNF-α, as well as insulin sensitivity, and to elucidate the possible relationship among the parameters and negative energy balance during the periparturient period of dairy cows. Material and Methods: Thirty primiparous Holstein dairy cows were selected for the study. Blood samples were collected from each cow seven days before the expected calving date, on the calving day, and 7, 14, and 21 days after calving. Blood non-esterified fatty acids (NEFA), β-hydroxybutyric acid (BHBA), glucose, insulin, adiponectin, and TNF-α levels were measured. Revised Quantitative Insulin Sensitivity Check Index (rQUICKI) was calculated using data on NEFA, insulin, and glucose concentrations. Results: When compared to prepartum levels, serum concentration of adiponectin significantly increased on day 21 postpartum. The rQUICKI increased and NEFA levels decreased on day 7 after parturition. Insulin and glucose levels decreased on days 7, 14, and 21 postpartum when compared with prepartum levels. BHBA levels decreased on day 21 and TNF-α concentration also decreased on days 7, 14, and 21 postpartum. Adiponectin levels positively correlated with NEFA during the preparturient period. Negative correlation was detected between adiponectin and rQUICKI on calving day and on 14 th day after parturition. TNF-α concentration positively correlated with glucose levels on day 7 prepartum and on 21 st day postpartum and with rQUICKI on 21 st day postpartum. Negative correlation was detected between adiponectin level and insulin sensitivity. Conclusion: Based on the results of the study, we concluded that adiponectin could possibly increase insulin sensitivity when blood NEFA concentrations are elevated.

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Section: Discussionsupporting

confidence: 88%

Changes in circulating adiponectin and tumour necrosis factor-α and their relationship with insulin resistance in periparturient dairy cows

Mecitoğlu

Şentürk

Akgül

et al. 2016

Journal of Veterinary Research

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“…It should be noted that the expression of ADIPOQ is not correlated with serum concentrations of adiponectin. Adiponectin production is mainly controlled by posttranscriptional factors (Lemor et al, 2009;Singh et al, 2014). Therefore, the lack of association between adipocyte size and ADIPOQ expression in the present study does not exclude a potential association between adiponectin concentration and fatness of the animals.…”

Section: Influence Of Adipocyte Size and Adipose Depot On Adipoq And mentioning

confidence: 55%

“…Adipocytes are the main source of circulating adiponectin and leptin (Chilliard et al, 2005;Singh et al, 2014). In humans and rodents, both adipokines play an important role in the regulation of energy homeostasis and immune function (Ingvartsen and Boisclair, 2001;Kadowaki and Yamauchi, 2005;Carbone et al, 2012).…”

Section: Influence Of Adipocyte Size and Adipose Depot On Adipoq And mentioning

confidence: 99%

“…Therefore, the lack of association between adipocyte size and ADIPOQ expression in the present study does not exclude a potential association between adiponectin concentration and fatness of the animals. Indeed, a negative correlation between serum adiponectin concentration and BCS has been demonstrated (Singh et al, 2014;De Koster et al, 2017). Besides its well-described role in the regulation of feed intake, leptin exerts proinflammatory effects by activating the inflammatory cascade in immune cells in humans and rodents (Ingvartsen and Boisclair, 2001;Paz-Filho et al, 2012).…”

Section: Influence Of Adipocyte Size and Adipose Depot On Adipoq And mentioning

confidence: 99%

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Influence of adipocyte size and adipose depot on the number of adipose tissue macrophages and the expression of adipokines in dairy cows at the end of pregnancy

Depreester

Koster

Poucke

et al. 2018

Journal of Dairy Science

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The aim of this study was to determine the number of adipose tissue macrophages (ATM) and the mRNA expression of adipokines [adiponectin (ADIPOQ), leptin (LEP), interleukin 6 (IL6), tumor necrosis factor (TNF), and interleukin 10 (IL10)] in different adipose depots from cows with a variable body condition score (BCS) at the end of the dry period. We hypothesized that the number of ATM and the expression of these adipokines depend on adipocyte size and the anatomical location of the adipose depot. Subcutaneous, omental, mesenteric, perirenal, and intrapelvic adipose tissue samples were taken immediately after euthanasia of 10 Holstein Friesian dairy cows (upcoming parity 2 to 5, age 3.9 ± 1.4 yr; mean ± standard deviation) at the end of pregnancy (actual days of pregnancy at the moment of euthanasia: 269 ± 5 d). During the dry period, all animals received similar diets to meet but not exceed requirements. Five animals were considered to have a normal BCS (2.5-3.5) and 5 animals were considered to be over-conditioned (BCS = 3.75-5). Body weight of the animals at the moment of euthanasia was 717 ± 77 kg. Expression of the different genes was determined by reverse transcription quantitative real-time PCR. Adipocyte size was determined by measuring the area of 100 adipocytes on histological sections. Average adipocyte area was 10,475 ± 1,019, 8,500 ± 780, 10,383 ± 1,227, 11,466 ± 1,039, and 11,087 ± 1,632 µm for the subcutaneous, mesenteric, omental, intrapelvic, and perirenal adipose depot, respectively. Immunohistochemistry using anti-bovine CD172a antibodies was performed to determine the proportion of ATM (the number of CD172a-positive cells per 100 adipocytes, given as a percentage). Expression of LEP, IL6, and TNF was positively associated with adipocyte size, whereas no association could be detected between ADIPOQ and IL10 with the size of the adipocytes. The omental adipose depot was especially infiltrated with ATM (1.92 ± 0.55, 1.10 ± 0.33, and 8.28 ± 2.24% for the subcutaneous, mesenteric, and omental adipose depot, respectively). The proportion of ATM was positively associated with the size of the adipocytes in the omental and mesenteric adipose depot. Expression of ADIPOQ, LEP, IL6, TNF, and IL10 differed among depots, which suggests differences in inflammatory characteristics depending on the anatomical location of depots. In conclusion, the results of the present study confirm the adipose tissue as a potential source of inflammatory mediators and demonstrate ATM infiltration, especially in the omental adipose depot.

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“…This dysregulated production is characterized by an increased production of proinflam-matory adipokines, such as tumor necrosis factor-α, resistin, IL6, and decreased production of adiponectin, an anti-inflammatory adipokine (Cornier et al, 2008). Recent investigations support an important role for resistin (Reverchon et al, 2014), adiponectin (Singh et al, 2014), tumor necrosis factor-α (Ohtsuka et al, 2001), and leptin (Ingvartsen and Boisclair, 2001) in the transition period of dairy cows. However, the underlying mechanism by which adipokines of enlarged adipocytes in overconditioned dairy cattle may influence insulin response of the glucose and FA metabolism requires further investigation.…”

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confidence: 99%

Insulin response of the glucose and fatty acid metabolism in dry dairy cows across a range of body condition scores

Koster

Hostens

Eetvelde

et al. 2015

Journal of Dairy Science

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The objective of the present research was to determine the insulin response of the glucose and fatty acid metabolism in dry dairy cows with a variable body condition score (BCS). Ten pregnant Holstein Friesian dairy cows (upcoming parity 2 to 5) were selected based on BCS at the beginning of the study (2mo before expected parturition date). During the study, animals were monitored weekly for BCS and backfat thickness and in the last 2wk, blood samples were taken for determination of serum nonesterified fatty acid (NEFA) concentration. Animals underwent a hyperinsulinemic euglycemic clamp test in the third week before the expected parturition date. The hyperinsulinemic euglycemic clamp test consisted of 4 consecutive insulin infusions with increasing insulin doses: 0.1, 0.5, 2, and 5mIU/kg per minute. For each insulin infusion period, a steady state was defined as a period of 30min where no or minor changes of the glucose infusion were necessary to keep the blood glucose concentration constant and near basal levels. During the steady state, the glucose infusion rate [steady state glucose infusion rate (SSGIR) in µmol/kg per minute] and NEFA concentration [steady state NEFA concentration (SSNEFA) in mmol/L] were determined and reflect the insulin response of the glucose and fatty acid metabolism. Dose response curves were created based on the insulin concentrations during the steady state and the SSGIR or SSNEFA. The shape of the dose response curves is determined by the concentration of insulin needed to elicit the half maximal effect (EC50) and the maximal SSGIR or the minimal SSNEFA for the glucose or fatty acid metabolism, respectively. The maximal SSGIR was negatively associated with variables reflecting adiposity of the cows (BCS, backfat thickness, NEFA concentration during the dry period, and absolute weight of the different adipose depots determined after euthanasia and dissection of the different depots), whereas the EC50 of the glucose metabolism was positively associated with these variables. These results reflect a decreased insulin sensitivity and a decreased insulin responsiveness of the glucose metabolism in overconditioned dry dairy cows. The minimal SSNEFA and the EC50 of the fatty acid metabolism were not associated with variables reflecting adiposity of the cows, meaning that the insulin response of the fatty acid metabolism was not associated with the level of fat accumulation in dry dairy cows. Additionally, within individual cows, the EC50 of the glucose metabolism was higher than the EC50 of the fatty acid metabolism, meaning that the response of the fatty acid metabolism occurs at lower insulin concentrations compared with the response of the glucose metabolism. It can be concluded that a negative association exists between the level of fat accumulation in pregnant dairy cows at the end of the dry period and the insulin response of the glucose metabolism.

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Lactation driven dynamics of adiponectin supply from different fat depots to circulation in cows

Cited by 21 publications

References 62 publications

Changes in circulating adiponectin and tumour necrosis factor-α and their relationship with insulin resistance in periparturient dairy cows

Changes in circulating adiponectin and tumour necrosis factor-α and their relationship with insulin resistance in periparturient dairy cows

Influence of adipocyte size and adipose depot on the number of adipose tissue macrophages and the expression of adipokines in dairy cows at the end of pregnancy

Insulin response of the glucose and fatty acid metabolism in dry dairy cows across a range of body condition scores

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