Lactate dehydrogenase isolated from human liver mitochondria: Its purification and partial biochemical characterization

Ketchum, C H; Robinson, C. Andrew; Hall, Leo M.; Grizzle, William E.

doi:10.1016/s0009-9120(88)80006-7

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…The latter finding can likely be attributed to the concurrent presence of different isoforms of LDH and MDH. For example, activity of LDH in mitochondria has been reported before [33] and since MDH1 and its mitochondrial isoform MHD2 exhibit the same substrate specificity, the acquired enzyme activity profile presumably reflects the activities of both isoforms. Altogether, our data support the idea that small portions of LDHA and MHD1 are indeed associated with human peroxisomes.…”

Section: Resultsmentioning

confidence: 87%

The Proteome of Human Liver Peroxisomes: Identification of Five New Peroxisomal Constituents by a Label-Free Quantitative Proteomics Survey

et al. 2013

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The peroxisome is a key organelle of low abundance that fulfils various functions essential for human cell metabolism. Severe genetic diseases in humans are caused by defects in peroxisome biogenesis or deficiencies in the function of single peroxisomal proteins. To improve our knowledge of this important cellular structure, we studied for the first time human liver peroxisomes by quantitative proteomics. Peroxisomes were isolated by differential and Nycodenz density gradient centrifugation. A label-free quantitative study of 314 proteins across the density gradient was accomplished using high resolution mass spectrometry. By pairing statistical data evaluation, cDNA cloning and in vivo colocalization studies, we report the association of five new proteins with human liver peroxisomes. Among these, isochorismatase domain containing 1 protein points to the existence of a new metabolic pathway and hydroxysteroid dehydrogenase like 2 protein is likely involved in the transport or β-oxidation of fatty acids in human peroxisomes. The detection of alcohol dehydrogenase 1A suggests the presence of an alternative alcohol-oxidizing system in hepatic peroxisomes. In addition, lactate dehydrogenase A and malate dehydrogenase 1 partially associate with human liver peroxisomes and enzyme activity profiles support the idea that NAD+ becomes regenerated during fatty acid β-oxidation by alternative shuttling processes in human peroxisomes involving lactate dehydrogenase and/or malate dehydrogenase. Taken together, our data represent a valuable resource for future studies of peroxisome biochemistry that will advance research of human peroxisomes in health and disease.

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Section: Resultsmentioning

confidence: 87%

The Proteome of Human Liver Peroxisomes: Identification of Five New Peroxisomal Constituents by a Label-Free Quantitative Proteomics Survey

et al. 2013

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“…236 He/Bremme/Kallner/Blomback LDH in Preeclampsia Other earlier reports asserted that the increase in LDH was associated with liver cell damage [3-6, 8, 9], since elevated ALT and AST concentrations were observed in preeclamptic women with high LDH activity. Some re searchers isolated LDH-5 which is one of the subunit compositions of LDH from liver tissues [10,12]. They found increased concentrations of LDH-5 in preeclamptic women with liver damage.…”

Section: Discussionmentioning

confidence: 99%

Increased Concentrations of Lactate Dehydrogenase in Pregnancy with Preeclampsia: A Predictor for the Birth of Small-for-Gestational-Age Infants

He¹,

Bremme

Kallner³

et al. 1995

Gynecol Obstet Invest

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Lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations and platelet counts were measured in 26 normal pregnant women and 51 preeclamptic women. In the normal-pregnancy group, no significant changes were found in the results of these tests. In the preeclampsia group, ALT and AST concentrations were not significantly higher than those in normal pregnancy, but the LDH concentrations increased and the platelet counts decreased significantly through the pregnancy. The increases in LDH did not correlate with changes in ALT or AST. Preeclamptic women with small-for-gestational-age (SGA) infants had significantly higher LDH concentrations than those in the appropriate-for-gestational-age (AGA) group, but ALT and AST concentrations did not increase significantly. As reasons for the LDH increase in our subjects, liver damage was excluded and more active glycolysis in addition to severe cell damage due to chronic anoxemia were inferred. It is suggested that an increase in LDH is predictive of SGA infants in preeclamptic pregnancy, especially in those with normal liver function.

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“…Therefore, significantly higher activities of these enzymes in PGE1 rabbits compared to IP and AD animals indicated less effective amelioration of liver IRI with PGE1 preconditioning in comparison with IP and AD. While ALT is localized solely in the cellular cytoplasm, AST and LDH are both cytosolic and mitochondrial enzymes (11,12). Hence, considering the significantly higher LDH serum activity in the IP group compared to the AD group, this would suggest a greater hepatocellular damage, including the release of both cytosolic and mitochondrial LDH by mitochondria in IP animals, i.e.…”

Section: Resultsmentioning

confidence: 99%

Ischemic preconditioning vs adenosine vs prostaglandin E1 for protection against liver ischemia/reperfusion injury

Radojković

Stojanović

Stanojević

et al. 2017

Braz J Med Biol Res

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Ischemia/reperfusion injury is still a major cause of morbidity and mortality during liver surgery and transplantation. A variety of surgical and pharmacological therapeutic strategies have been investigated to minimize the effects of ischemia/reperfusion. The aim of our study was to analyze and compare preventive influences of ischemic preconditioning, adenosine and prostaglandin E1 in the experimental model of hepatic ischemia/reperfusion injury. Adult chinchilla rabbits were divided into four groups: 10 rabbits subjected to liver ischemic preconditioning (3-min period of inflow occlusion followed by a 5-min period of reperfusion) followed by 45 min of Pringle maneuver; 10 rabbits subjected to pre-treatment with intraportal injection of adenosine followed by 45 min of Pringle maneuver; 10 rabbits subjected to pre-treatment with intraportal injection of prostaglandin E1 followed by 45 min of Pringle maneuver; and control group of 10 rabbits subjected to 45 min of inflow liver ischemia without any preconditioning. On the second postoperative day, blood samples were obtained and biochemical parameters of liver function were measured and compared. Liver tissue samples were also obtained and histopathological changes were compared. Based on biochemical and histopathological parameters, it was demonstrated that ischemic preconditioning provided the best protection against hepatic ischemia/reperfusion injury. This was probably due to a wider range of mechanisms of action of this method oriented to reduce oxidative stress and inflammation, and restore liver microcirculation and hepatocyte energy compared to the examined pharmacological strategies.

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Lactate dehydrogenase isolated from human liver mitochondria: Its purification and partial biochemical characterization

Cited by 10 publications

References 12 publications

The Proteome of Human Liver Peroxisomes: Identification of Five New Peroxisomal Constituents by a Label-Free Quantitative Proteomics Survey

The Proteome of Human Liver Peroxisomes: Identification of Five New Peroxisomal Constituents by a Label-Free Quantitative Proteomics Survey

Increased Concentrations of Lactate Dehydrogenase in Pregnancy with Preeclampsia: A Predictor for the Birth of Small-for-Gestational-Age Infants

Ischemic preconditioning vs adenosine vs prostaglandin E1 for protection against liver ischemia/reperfusion injury

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