Lactate dehydrogenase isoenzymes in odontoblasts from rat incisors

Linde, Lise; Ljunggren, Anna

doi:10.1016/0003-9969(70)90107-x

Cited by 4 publications

(3 citation statements)

References 3 publications

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“…The findings of an increased activity of lactate dehydrogenase and eytoehrome oxidase in the low calcium and vitamin D deficient rat compared to the normal rat would thus be in agreement wtih this. As judged by the LDH iso-enzyme pattern and inhibition of LDH by pyruvate, this increase in energy metabolism did not seem to be accompanied by any shift from the predominant aerobic pattern (Linde & Ljunggren 1970).…”

Section: Discussionmentioning

confidence: 78%

“…The iso-enzyme pattern of lactate dehydrogenase (LDH) in the odontoblast material was investigated by cellulose acetate membrane electrophoresis (Fig. 1), Sitnilar to the results found by Linde &. Ljunggren (1970), LDH 1 (H^) was the predotninant iso-enzyme in odontoblasts from rats fed the control diet (R 47) (Fig, 1).…”

Section: Lactate Dehydrogenese Characterizationmentioning

confidence: 75%

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Odontoblast metabolism in rats deficient in vitamin D and calcium. IV. Lysosomal and energy metabolic enzymes

Engström

1980

J Oral Pathology Medicine

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Young rats, fed a low calcium and vitamin D deficient diet for 2 weeks, developed hypocalcemia, an increased activity of scrum alkaline phosphatase and an increase in the serum concentration of immunoreactive parathyroid hormone. An increased activity of lactate dehydrogenase and cytochrome oxidase in odontoblasts was found. No shift in the general energy metabolic pathway was found as visualized in the lactate dehydrogenase iso‐enzyme pattern. The dominating lactate dehydrogenase iso‐enzyme in odontoblasts from both the normal and the deficient rats was LDH I (H4, LD5), thus indicating primarily an aerobic energy‐metabolism. Also the activities of the lysosomal enzymes acid phosphatase, cathepsin D and hyaluronidase in the odontoblasts from the deficient animals were increased when compared to the normal animals. No significant change could be demonstrated for β‐glucuronidase and β‐N‐acetylglucosaminidase. It was earlier found that this deficient diet caused an increase in odontoblast alkaline phosphatase activities and protein synthesis in vitro. In view of the present findings it might be concluded that the low calcium and vitamin D deficient diet causes a general increase in the odontoblast metabolism. It is not known whether this is due to the increase in parathyroid hormone or if it is a direct effect of the lowered serum calcium concentration.

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Section: Discussionmentioning

confidence: 78%

Section: Lactate Dehydrogenese Characterizationmentioning

confidence: 75%

Odontoblast metabolism in rats deficient in vitamin D and calcium. IV. Lysosomal and energy metabolic enzymes

Engström

1980

J Oral Pathology Medicine

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“…and has suggested that these respiratory fluctuations merely reflect in vivo altera tions between anaerobic and aerobic path ways. Linde and Ljunggren [1970] have demonstrated the predominance of a strictly aerobic metabolism in the odontoblasts dur ing primary dentinogenesis by electrophoretically characterizing the pattern of lactate dehydrogenase (LDH; EC 1.1.1.27) isoen zyme distribution. The extent to which met abolic pathways in connective tissues are aerobie or anaerobic seems to depend on the oxygen supply to the tissue.…”

mentioning

confidence: 99%

Variable Ratios of Lactate and Malate Dehydrogenase-Like Enzyme Activities and LDH Isoenzyme Distribution in the Odontoblast-Predentine Region of Intact and Carious Human Teeth

Karjalainen¹,

Mäkinen²

1980

Caries Res

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Coronal odontoblast-predentine tissue was collected from intact teeth and from teeth with various types of occlusal caries. Lactate (LDH) and malate (MDH) dehydrogenase-like activities in the non-sedimentable (20,200 g), saline-soluble tissue extracts were measured. The calculated LDH/MDH ratio was higher (p < 0.050) in teeth with initial dentine caries (0.81) than in intact teeth (0.67). However, the ratios remained below 1.0 in all types of the tissues analyzed, suggesting a predominantly aerobic metabolism in the odontoblast-predentine region. In the enamel and in the initial dentine caries groups as well as in the group of teeth restored with amalgam, both the LDH and MDH values remained near to the level of intact teeth. The further development of the various lesion was associated with a gradual decrease in the dehydrogenase-like activities. This decrease became significant in recurrent carious lesions. The observation reassured the previously presented concept that a significant depression of several metabolic events occurs in the area of severe injury. Electrophoresis of odontoblast-predentine extracts revealed three anodally migrating LDH isoenzymes. Among these, LDH₂ and LDH₃ were the most prominent, both with intact and carious samples. This was thought to give further evidence of the aerobic pattern of metabolism suggested to predominate in the area.

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A method for the biochemical study of enzymes in the rat odontoblast layer during dentinogenesis

Linde

1972

Archives of Oral Biology

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Lactate dehydrogenase isoenzymes in odontoblasts from rat incisors

Cited by 4 publications

References 3 publications

Odontoblast metabolism in rats deficient in vitamin D and calcium. IV. Lysosomal and energy metabolic enzymes

Odontoblast metabolism in rats deficient in vitamin D and calcium. IV. Lysosomal and energy metabolic enzymes

Variable Ratios of Lactate and Malate Dehydrogenase-Like Enzyme Activities and LDH Isoenzyme Distribution in the Odontoblast-Predentine Region of Intact and Carious Human Teeth

A method for the biochemical study of enzymes in the rat odontoblast layer during dentinogenesis

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