Variable Ratios of Lactate and Malate Dehydrogenase-Like Enzyme Activities and LDH Isoenzyme Distribution in the Odontoblast-Predentine Region of Intact and Carious Human Teeth

Abstract

Coronal odontoblast-predentine tissue was collected from intact teeth and from teeth with various types of occlusal caries. Lactate (LDH) and malate (MDH) dehydrogenase-like activities in the non-sedimentable (20,200 g), saline-soluble tissue extracts were measured. The calculated LDH/MDH ratio was higher (p < 0.050) in teeth with initial dentine caries (0.81) than in intact teeth (0.67). However, the ratios remained below 1.0 in all types of the tissues analyzed, suggesting a predominantly aerobic metabolism in the odontoblast-predentine region. In the enamel and in the initial dentine caries groups as well as in the group of teeth restored with amalgam, both the LDH and MDH values remained near to the level of intact teeth. The further development of the various lesion was associated with a gradual decrease in the dehydrogenase-like activities. This decrease became significant in recurrent carious lesions. The observation reassured the previously presented concept that a significant depression of several metabolic events occurs in the area of severe injury. Electrophoresis of odontoblast-predentine extracts revealed three anodally migrating LDH isoenzymes. Among these, LDH₂ and LDH₃ were the most prominent, both with intact and carious samples. This was thought to give further evidence of the aerobic pattern of metabolism suggested to predominate in the area.

Production and application of purified mutanase from novel Cellulosimicrobium funkei SNG1 in the in vitro biofilm degradation

“…5 Mutanases, also known as α-1,3-glucanases (EC 3.2.1.59), are extracellular inducible enzymes that hydrolyze α-1,3-glucosidic bonds and can be used to reduce dental plaque formation. 6 Mutanases have been found in Bacillus, Paenibacillus, Flavobacterium, Streptomyces, Pseudomonas bacteria, and fungi, most prominently Trichoderma. [7][8][9][10][11] Production of mutanases mainly depends on inducer mutan and its structural heterogeneity.…”

“…In recent years, usage of microbial enzymes in toothpaste and mouthwashes to reduce dental plaque and maintain oral hygiene has garnered considerable attention 5 . Mutanases, also known as α‐1,3‐glucanases (EC 3.2.1.59), are extracellular inducible enzymes that hydrolyze α‐1,3‐glucosidic bonds and can be used to reduce dental plaque formation 6 . Mutanases have been found in Bacillus , Paenibacillus , Flavobacterium , Streptomyces , Pseudomonas bacteria, and fungi, most prominently Trichoderma 7–11 …”