Lactam inhibiting Streptococcus mutans growth on titanium

Xavier, Jhunior; Geremias, Thaise C.; Montero, Juan Felipe Dumes; Vahey, Brendan R.; Benfatti, César Augusto Magalhães; Souza, Júlio C. M.; Magini, Ricardo de Souza; Pimenta, Aparecida Linhares

doi:10.1016/j.msec.2016.07.013

Cited by 15 publications

(19 citation statements)

References 26 publications

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“…Likewise, immobilization of a 15mer lasioglossin LL-III to silicone catheters conferred antibiofilm ability to the device [50]. Recently, Xavier et al showed that synthetic lactams could also inhibit the growth of Streptococcus mutans on titanium [51]. Our design of shorter AMPs (8mers) for coating has a practical consequence because such peptides can be made more cost effectively than long peptides.…”

Section: Discussionmentioning

confidence: 99%

Design and surface immobilization of short anti-biofilm peptides

et al. 2017

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Short antimicrobial peptides are essential to keep us healthy and their lasting potency can inspire the design of new types of antibiotics. This study reports the design of a family of eight-residue tryptophan-rich peptides (TetraF2W) obtained by converting the four phenylalanines in temporin-SHf to tryptophans. The temporin-SHf template was identified from the antimicrobial peptide database (http://aps.unmc.edu/AP). Remarkably, the double arginine variant (TetraF2W-RR) was more effective in killing methicillin-resistant Staphylococcus aureus (MRSA) USA300, but less cytotoxic to human skin HaCat and kidney HEK293 cells, than the lysine-containing dibasic combinations (KR, RK and KK). Killing kinetics and fluorescence spectroscopy suggest membrane targeting of TetraF2W-RR, making it more difficult for bacteria to develop resistance. Because established biofilms on medical devices are difficult to remove, we chose to covalently immobilize TetraF2W-RR onto the polyethylene terephthalate (PET) surface to prevent biofilm formation. The successful surface coating of the peptide is supported by FT-IR and XPS spectroscopies, chemical quantification, and antibacterial assays. This peptide-coated surface indeed prevented S. aureus biofilm formation with no cytotoxicity to human cells. In conclusion, TetraF2W-RR is a short Trp-rich peptide with demonstrated antimicrobial and anti-biofilm potency against MRSA in both the free and immobilized forms. Because these short peptides can be synthesized cost effectively, they may be developed into new antimicrobial agents or used as surface coating compounds.

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Section: Discussionmentioning

confidence: 99%

Design and surface immobilization of short anti-biofilm peptides

et al. 2017

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“…Some non-peptide agents also have this property. For instances, Xavier et al (2016) found that lactam can inhibit Streptococcus mutans growth on titanium. In addition, KR-12, the smallest antibacterial peptide (i.e., residues 18–29) of human LL-37, retains activity against E. coli K12, but not S. aureus USA300 (Wang 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Such infections can cause implant failures, increase treatment costs, and lead to high morbidity and mortality (Zhao et al 2009; Xavier et al 2016). Therefore, the best strategy is to prevent such infections (Costa et al 2011; Onaizi and Leong 2011; Mishra et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Titanium surfaces immobilized with the major antimicrobial fragment FK-16 of human cathelicidin LL-37 are potent against multiple antibiotic-resistant bacteria

Mishra

Wang

2017

Biofouling

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Infections on implanted medical devices are a challenging problem, especially when bacteria form difficult-to-treat biofilms. Antimicrobial peptides are considered as a solution due to their potency against antibiotic-resistant superbugs. Previously, we demonstrated the prevention of staphylococcal biofilm formation in an animal catheter model by injecting merecidin (formerly known as 17BIPHE2), a peptide engineered based on the only human cathelicidin. This study documents an alternative solution via covalent immobilization of FK-16, amino acid sequence FKRIVQRIKDFLRNLV-amide, which corresponds to the major antimicrobial region (residues 17–32) of LL-37. FK-16 is superior to the longer peptide LL-37 in terms of synthesis cost and the shorter peptide KR-12 in terms of activity spectrum. Indeed, the FK16-coated titanium surface showed a broad-spectrum activity against the ESKAPE pathogens, including Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species. It also demonstrated anti-adhesion and biofilm inhibition capabilities against both S. aureus and E. coli.

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“…Most of the studies report that the BG antibacterial effect against specific planktonic bacteria do not reflect realistic conditions of biofilm growth and pathogenicity at infected areas . Biofilm is a well‐organized microbial community embedded in an extracellular polymeric matrix composed of polysaccharides, nucleic acids, proteins and water, that adheres to different surfaces such as teeth, rehabilitation synthetic materials, bone, and soft tissues . As a result of a complex well‐organized structure, bacteria embedded in biofilm is 1000 times more resistant to antibiotic therapy compared to planktonic bacteria .…”

Section: Introductionmentioning

confidence: 99%

Inhibition of multi‐species oral biofilm by bromide doped bioactive glass

Galárraga-Vinueza

Passoni

et al. 2017

J Biomedical Materials Res

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Bioactive glass is an attractive biomaterial that has shown excellent osteogenic and angiogenic effects for oral bone repairing procedures. However, anti-biofilm potential related to such biomaterial has not been completely validated, mainly against multi-species biofilms involved in early tissue infections. The aim of the present study was to evaluate the anti-biofilm effect of 58 S bioactive glass embedding calcium bromide compounds at different concentrations. Bioactive glass free or containing 5, or 10 wt % CaBr was synthesized by alkali sol-gel method and then characterized by physco-chemical analyses and scanning electron microscopy (SEM). Then, samples were tested by microbiological assays using optical density, real time q-PCR, and SEM. Bioactive glass particles showed accurate chemical composition and an angular shape with a bimodal size distribution ranging from 0.6 to 110 µm. The mean particle size was around 29 µm. Anti-biofilm effect was recorded for 5 wt % CaBr -doped bioactive glass against S. mitis, V. parvula, P. gingivais, S. gordoni, A. viscosus, F, nucleatum, P. gingivais. F. nucleatum, and P. gingivalis. Such species are involved in the biofilm structure related to infections on hard and soft tissues in the oral cavity. The incorporation of calcium bromide into bioactive glass can be a strategy to enhance the anti-biofilm potential of bioactive glasses for bone healing and infection treatment. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1994-2003, 2017.

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Lactam inhibiting Streptococcus mutans growth on titanium

Cited by 15 publications

References 26 publications

Design and surface immobilization of short anti-biofilm peptides

Design and surface immobilization of short anti-biofilm peptides

Titanium surfaces immobilized with the major antimicrobial fragment FK-16 of human cathelicidin LL-37 are potent against multiple antibiotic-resistant bacteria

Inhibition of multi‐species oral biofilm by bromide doped bioactive glass

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