“…Either reason is consistent with proliferation arising from antigen presented by lacrimal gland epithelial cells. Proliferation in the autologous mixed cell reaction was also blocked by anti-MHC II, but not anti-MHC I antiserum (Guo et al, 2000). All these data support the underlying hypothesis.…”
Section: Proliferation In the Autologous Mixed Cell Reactionmentioning
confidence: 63%
“…The other method uses nylon mesh to separate the cell types, and yields a preparation of more highly-puri®ed lacrimal gland epithelial cells essentially free of immune-system cells (pLGEC). Once obtained, both preparations were cultured in PCM medium (Guo et al, 2000).…”
“…Either reason is consistent with proliferation arising from antigen presented by lacrimal gland epithelial cells. Proliferation in the autologous mixed cell reaction was also blocked by anti-MHC II, but not anti-MHC I antiserum (Guo et al, 2000). All these data support the underlying hypothesis.…”
Section: Proliferation In the Autologous Mixed Cell Reactionmentioning
confidence: 63%
“…The other method uses nylon mesh to separate the cell types, and yields a preparation of more highly-puri®ed lacrimal gland epithelial cells essentially free of immune-system cells (pLGEC). Once obtained, both preparations were cultured in PCM medium (Guo et al, 2000).…”
“…The lacrimal gland from three female New Zealand White rabbits (weighing ,4 kg) from Irish Farms (Norco, CA) were collected, minced and digested to isolate the interstitial cells as in previously established methods (Guo et al, 2000). Interstitial cells were frozen and sent to Epitomics Inc. (Burlingame, CA) for fusion with a proprietary rabbit myeloma cell line.…”
SummaryDespite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not wellcharacterized. This study identifies a transcytotic pathway in LGACs regulated by Rab11a for polymeric immunoglobulin receptor (pIgR) and dIgA. EGFP-tagged Rab11a expressed in primary LGACs labeled a unique membrane compartment of comparable localization to endogenous Rab11a beneath the apical plasma membrane. This compartment was enriched in pIgR and clearly distinct from the regulated secretory pathway. Comparison of dIgA uptake in LGACs expressing wild type and dominant negative EGFPRab11a showed that the rapid exocytosis of dIgA was inhibited in acini expressing the dominant-negative protein, which additionally redistributed subapical pIgR. The trafficking of EGFP-Rab11a-enriched vesicles was regulated by microtubule-based and myosin Vb motors at distinct steps. Our data suggest that Rab11a is a crucial regulator of dIgA trafficking in primary acinar secretory epithelial cells and further support a role for microtubules, cytoplasmic dynein, actin filaments and myosin Vb in the maintenance of the Rab11a compartment in this primary secretory epithelial cell.
“…Subsequent experiments were conducted with lacrimal acinar cells prepared for culture by the method of Guo et al (2000). To determine the effect of time of exposure to retinoic acid on AR mRNA expression, cells were exposed to 10 À6 M retinoic acid for 4±24 hr.…”
Section: Effect Of Retinoic Acid On Ar Mrna Expressionmentioning
confidence: 99%
“…1, a modi®cation and improvement of the Rismondo procedure was published by Guo et al (2000) and Scho Ènthal et al (2000). Subsequent experiments were conducted using cells prepared by this method, which has been described in detail and was followed without modi®cation.…”
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