Autologous Lacrimal–Lymphoid Mixed-cell Reactions Induce Dacryoadenitis in Rabbits

Growth of Purified Lacrimal Acinar Cells in Matrigel Raft Cultures

“…Many recent studies have noted the bene®t of seeding preparations of lacrimal acinar cells onto Matrigel-coated culture plates (Kelleher et al, 1991;Meneray et al, 1994;Millar et al, 1996;Chen et al, 1998). Recent studies by the authors group have established procedures for the isolation of highly puri®ed preparations of lacrimal acinar cells (Guo et al, 2000) and methods which enhance the proliferation of the lacrimal acinar cells (Scho Ènthal et al, 2000). Prior to this time lacrimal acinar cells could be established and maintained in culture for short periods of time, approximately 7 days, with minimal proliferation.…”

Lacrimal Gland Epithelial Cells Stimulate Proliferation in Autologous Lymphocyte Preparations

“…Also consistent with this hypothesis are the observations that intact but not lysed pLGEC stimulated proliferation of autologous lymphocytes in an autologous mixed cell reaction, and that intact but not lysed pLGEC created a cell reaction that reliably induced dacryoadenitis (Guo et al, 2000). These results suggest that studies of pLGEC may help elucidate mechanisms that lead to autoimmune dry eye, and thereby help identify new therapies for the disease.…”

“…The MHC class II cycle between the basal-lateral plasma membranes and endomembrane compartments , and certain of the endomembrane compartments through which they traf®c contain both cathepsins and La/SSb proteins (Yang et al, 1999). The hypothesis is further supported by observations that proliferation does not occur when lymphocytes are cocultured in a Transwell system that prevents direct contact between the two cell types (Guo et al, 2000). FIG.…”

Tissue‐engineered tear secretory system: Functional lacrimal gland acinar cells cultured on matrix protein‐coated substrata

“…16 Briefly, inferior lacrimal glands were removed aseptically, placed in a dish with Ham's complete medium, and finely minced with a pair of scalpel blades (No.18). Ham's complete medium consists of Ham's F-12 supplemented with 100 U mL Ϫ1 pencillin, 0.1 mg mL Ϫ1 streptomycin, 2 mM glutamine, 2 mM butyrate, 0.084 mg L Ϫ1 linoleic acid, 0.05 mg mL Ϫ1 trypsin inhibitor, 10 mM hepes, and 5 mg mL Ϫ1 BSA.…”