Lack of β-subunit of β-conglycinin in non-nodulating isolines of soybean

Ohtake, Norikuni; Nishiwaki, Toshikazu; Mizukoshi, Kazushi; Chinushi, Toshiaki; Ohyama, Takuji

doi:10.1080/00380768.1994.10413309

Cited by 18 publications

(4 citation statements)

References 10 publications

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“…β-conglycinin is comprised of three subunits, designated as α', α, and β−subunits [46]. We happened to discover the lack of β-subunit of β-conglycinin in several non-nodulated soybean lines, although an electrophoretic protein band due to this protein was clearly detected in the corresponding nodulated isolines [47]. The suppression of the β−subunit in the non-nodulating isoline T201 is regulated at the level of mRNA accumulation.…”

Section: Nitrogen Metabolism In Soybean Leavesmentioning

confidence: 99%

Soybean Seed Production and Nitrogen Nutrition

Ohyama¹,

Minagawa²,

Ishikawa³

et al. 2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

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Section: Nitrogen Metabolism In Soybean Leavesmentioning

confidence: 99%

Soybean Seed Production and Nitrogen Nutrition

Ohyama¹,

Minagawa²,

Ishikawa³

et al. 2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

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“…The possibility that nitrogen nutrition plays a role in sulfur-de®ciency responses has also been suggested. Ohtake et al (1994) reported that seed storage-protein composition was altered in nonnodulating soybean mutants, and that little or no accumulation of the b-subunit of b-conglycinin could be observed in the mutants. As they were unable to ®x nitrogen, nitrogen supply to the mutant plants was greatly reduced and the relative sulfur supply was therefore higher than in the wild-type plants.…”

Section: Introductionmentioning

confidence: 98%

Role of O -acetyl- l -serine in the coordinated regulation of the expression of a soybean seed storage-protein gene by sulfur and nitrogen nutrition

Kim

Hirai

Hayashi

et al. 1999

Planta

131

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The composition of seed storage proteins is regulated by sulfur and nitrogen supplies. Under conditions of a low sulfur-to-nitrogen ratio, accumulation of the beta-subunit of beta-conglycinin, a sulfur-poor seed storage protein of soybean (Glycine max [L.] Merr.), is elevated, whereas that of glycinin, a sulfur-rich storage protein, is reduced. Using transgenic Arabidopsis thaliana [L.] Heynh., it was found that the promoter from the gene encoding the beta-subunit of beta-conglycinin up-regulates gene expression under sulfur deficiency and down-regulates gene expression under nitrogen deficiency. To obtain an insight into the metabolic control of this regulation, the concentrations of metabolites related to the sulfur assimilation pathway were determined. Among the metabolites, O-acetyl-l-serine (OAS), one of the precursors of cysteine biosynthesis, accumulated to higher levels under low-sulfur and high-nitrogen conditions in siliques of transgenic A. thaliana. The pattern of OAS accumulation in response to various levels of sulfur and nitrogen was similar to that of gene expression driven by the beta-subunit promoter. Elevated levels of OAS accumulation were also observed in soybean cotyledons cultured under sulfur deficiency. Moreover, OAS applied to in-vitro cultures of immature soybean cotyledons under normal sulfate conditions resulted in a high accumulation of the beta-subunit mRNA and protein, whereas the accumulation of glycinin was reduced. These changes were very similar to the responses observed under conditions of sulfur deficiency. Our results suggest that the level of free OAS mediates sulfur- and nitrogen-regulation of soybean seed storage-protein composition.

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“…Free amino acids were extracted three times with 80% ethanol and the contents were determined using the ninhydrin method (Ohyama 1990), while the contents of free sugars were determined using the phenol-sulfuric acid method (Osaki 1990). Protein extraction and sodium dodecylsulfate-polyacrylamide gel electrophoresis were carried out as previously described (Ohtake et al 1994). The gels were stained with Coomassie Brilliant Blue G-250 and the peak area of each subunit was quantified by scanning the gel by densitometry at A595.…”

Section: Observation and Analysismentioning

confidence: 99%

Effect of basal side-dressing of various types of coated urea fertilizer on shoot growth, yield components and seed composition of soybean (Glycine max(L.) Merr.)

Ohtake

Hattori

Nagumo

et al. 2006

Soil Science and Plant Nutrition

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Effect of basal side-dressing ORIGINAL ARTICLE Effect of basal side-dressing of various types of coated urea fertilizer on shoot growth, yield components and seed composition of soybean (Glycine max (L.) Merr.) Yoshihiko AbstractApplication of nitrogen (N) fertilizer at different times affected the shoot characters, yield components and seed composition of the soybean cultivar "Enrei" and its non-nodulating line "En1282". Soybean plants were cultivated using various types of coated urea fertilizers for controlled released of N. The control treatment consisted of basal side-dressing of readily available mixed fertilizer, which contained 1.6 g N m . Various types of coated urea fertilizers were applied with basal sidedressing of 6 g N m −2 in addition to the mixed fertilizer. The types of coated urea fertilizers were as follows: linear release types CU70, CU100 and CU140, and sigmoid release types CUS80, CUS100 and CUS120. A major part of the N from CU70 and CU100 was released before the middle-stage of growth (beginning of pod stage), whereas CUS80 and CUS100 released N at approximately the middle-stage and CUS120 released N after the mid-stage. CU140 fertilizer is a uniform release type throughout the growth period. At the middle stage of growth, the chlorophyll content (leaf color) of En1282 was high with CU100 treatment, while the CUS100 treatment led to high chlorophyll content during the filling period. Leaf chlorophyll content of Enrei tended to increase with the CUS120 treatment during the filling period. The N 2 fixation activity of nodulating Enrei, which was estimated using the relative ureide method, significantly decreased in the CU70, CU100 and CU140 treatments compared with the control at the flowering stage. At the beginning of the pod stage, treatment with CUS120 tended to lead to a higher N 2 fixation activity than the control, whereas the CUS80 and CUS100 treatments depressed the N 2 fixation activity during the seed filling period. The amount of N that accumulated in the shoots in all the coated urea treatments increased significantly compared with the control treatment in En1282 at the beginning of the maturity stage, whereas in Enrei it increased significantly only in CUS120 compared with the control. The yield was significantly higher in all the coated urea treatments compared with the control (49 g m ) in which N supply from the flowering period to the beginning of the pod stage resulted in the highest yield in En1282. In addition, in Enrei the seed yield increased significantly in each coated urea treatment and the largest increase was induced by CUS120 (498 g m ). In non-nodulating En1282, the amount of N released from flowering to the beginning of the pod stages showed a significant correlation with the total number of nodes, seed yield and seed weight. In nodulating Enrei, there was a significant relationship between N release from the beginning of the pod to the beginning of the maturity stages and the length of the main stem, seed yield and seed protein content. There was no signif...

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Lack of β-subunit of β-conglycinin in non-nodulating isolines of soybean

Cited by 18 publications

References 10 publications

Soybean Seed Production and Nitrogen Nutrition

Soybean Seed Production and Nitrogen Nutrition

Role of O -acetyl- l -serine in the coordinated regulation of the expression of a soybean seed storage-protein gene by sulfur and nitrogen nutrition

Effect of basal side-dressing of various types of coated urea fertilizer on shoot growth, yield components and seed composition of soybean (Glycine max(L.) Merr.)

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