Lack of Vertical Transmission of Severe Acute Respiratory Syndrome Coronavirus 2, China

Li, Yang; Zhao, Ruihong; Zheng, Shufa; Xu, Chen; Wang, Jinxi; Sheng, Xiaoli; Zhou, Jianying; Cai, Hongliu; Fang, Qiang; Yu, Fei; Fan, Jian Gao; Xu, Kaijin; Chen, Yu; Sheng, Jifang

doi:10.3201/eid2606.200287

Cited by 339 publications

(450 citation statements)

References 6 publications

Supporting

Mentioning

410

Contrasting

Unclassified

Order By: Relevance

“…At least five neonates born to pregnant women with COVID-19 pneumonia were reported to test positive for SARS-CoV-2 infection after birth [16][17][18] and other studies report newborns with elevated virus-specific antibodies to SARS-CoV-2 born to mothers with COVID-19 16,19 . However, several other studies have thus far failed to find evidence for intrauterine transmission from pregnant women with COVID-19 to their newborns in cohorts as large as 38 patients [20][21][22] . Additionally, newborns from 9 COVID-19 patients who had cesarean deliveries in their third trimester tested negative for SARS-CoV-2 17 .…”

Section: Introductionmentioning

confidence: 99%

Integrated analyses of single-cell atlases reveal age, gender, and smoking status associations with cell type-specific expression of mediators of SARS-CoV-2 viral entry and highlights inflammatory programs in putative target cells

Muus

Luecken

Eraslan

et al. 2020

Preprint

250

326

View full text Add to dashboard Cite

The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, creates an urgent need for identifying molecular mechanisms that mediate viral entry, propagation, and tissue pathology. Cell membrane bound angiotensin-converting enzyme 2 (ACE2) and associated proteases, transmembrane protease serine 2 (TMPRSS2) and Cathepsin L (CTSL), were previously identified as mediators of SARS-CoV2 cellular entry. Here, we assess the cell type-specific RNA expression of ACE2, TMPRSS2, and CTSL through an integrated analysis of 107 single-cell and single-nucleus RNA-Seq studies, including 22 lung and airways datasets (16 unpublished), and 85 datasets from other diverse organs. Joint expression of ACE2 and the accessory proteases identifies specific subsets of respiratory epithelial cells as putative targets of viral infection in the nasal passages, airways, and alveoli. Cells that co-express ACE2 and proteases are also identified in cells from other organs, some of which have been associated with COVID-19 transmission or pathology, including gut enterocytes, corneal epithelial cells, cardiomyocytes, heart pericytes, olfactory sustentacular cells, and renal epithelial cells. Performing the first meta-analyses of scRNA-seq studies, we analyzed 1,176,683 cells from 282 nasal, airway, and lung parenchyma samples from 164 donors spanning fetal, childhood, adult, and elderly age groups, associate increased levels of ACE2, TMPRSS2, and CTSL in specific cell types with increasing age, male gender, and smoking, all of which are epidemiologically linked to COVID-19 susceptibility and outcomes. Notably, there was a particularly low expression of ACE2 in the few young pediatric samples in the analysis. Further analysis reveals a gene expression program shared by ACE2 + TMPRSS2 + cells in nasal, lung and gut tissues, including genes that may mediate viral entry, subtend key immune functions, and mediate epithelial-macrophage cross-talk. Amongst these are IL6, its receptor and co-receptor, IL1R, TNF response pathways, and complement genes. Cell type specificity in the lung and airways and smoking effects were conserved in mice. Our analyses suggest that differences in the cell type-specific expression of mediators of SARS-CoV-2 viral entry may be responsible for aspects of COVID-19 epidemiology and clinical course, and point to putative molecular pathways involved in disease susceptibility and pathogenesis.

show abstract

Section: Introductionmentioning

confidence: 99%

Integrated analyses of single-cell atlases reveal age, gender, and smoking status associations with cell type-specific expression of mediators of SARS-CoV-2 viral entry and highlights inflammatory programs in putative target cells

Muus

Luecken

Eraslan

et al. 2020

Preprint

250

326

View full text Add to dashboard Cite

show abstract

“…The four case reports (33.3%) [13,14,15] addressed women in the third trimester of pregnancy hospitalized with signs and symptoms of COVID-19, which was later confirmed with laboratory tests. In general, the studies describe the main changes affecting pregnant women who tested positive for COVID-19, while fever was the most common clinical symptom [13,14,15]. Lymphopenia [13,15] stood out among laboratory results along with pulmonary abnormalities on imaging exams [13,14,15].…”

Section: Resultsmentioning

confidence: 85%

“…In general, the studies describe the main changes affecting pregnant women who tested positive for COVID-19, while fever was the most common clinical symptom [13,14,15]. Lymphopenia [13,15] stood out among laboratory results along with pulmonary abnormalities on imaging exams [13,14,15]. Diagnoses were mainly determined by pharyngeal swab [13,14].…”

Section: Resultsmentioning

confidence: 99%

“…Most pregnant women (97.4%) were diagnosed before hospitalization and their primary symptoms and signs were fever, either before admission [13,14,15,16,17,18,21,23] or postpartum [13,14,15,16,17,20,21,23]; cough [15,16,17,18,20,21,22,23], dyspnea [14,15,16,17,18,21] and fatigue [16,23]. Note that three women were asymptomatic [16,21] but were tested (through RT-PCR to detect SARS-CoV-2) due to exposure to people diagnosed with COVID-19.…”

Section: Discussionmentioning

confidence: 99%

“…In terms of imaging exams, CT scan was widely employed and was useful to perform an initial evaluation at admission, while 73 of the pregnant women presented a chest CT scan suggestive of infection [13,14,15,16,17,18,20,21,22]. C-reactive protein levels were above In terms of the type of child delivery, most women underwent C-sections [13,14,15,16,17,18,19,20,21,22,23]; only four women had a normal delivery [20,21]. Recent literature tends to lean toward C-sections; however, much depends on women's comorbidities.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of COVID-19 infection during pregnancy and neonatal prognosis: what is the evidence?

Sousa

Carvalho

Oliveira

et al. 2020

Preprint

View full text Add to dashboard Cite

Background:Little is known about how COVID-19 infection affects pregnant women, as well as about the possibility of vertical transmission or that of premature labor. Thus, this study's objective was to identify existing evidence to directive public policies.Method: Systematic literature review assessing papers published in the most comprehensive databases in the field of health.Results: 12 papers were eligible. A total of 119 pregnant women aged between 22 and 42 years old and between the 12th and 41st weeks were analyzed; 90 children were born. Most : medRxiv preprint pregnant women acquired the infection in the third trimester of pregnancy, 97.4% were diagnosed before hospitalization based on signs and symptoms. There was no maternal death and no vertical transmission was confirmed.Conclusions: pregnant women must be cautious and vigilant because their exposure to the virus, even if at the end of a pregnancy, has important repercussions, especially for the type of labor.

show abstract

COVID‐19 in Pediatrics

Ulloa¹,

He²,

Chung³

et al. 2022

Coronavirus Disease 2019 (COVID‐19)

View full text Add to dashboard Cite

Lack of Vertical Transmission of Severe Acute Respiratory Syndrome Coronavirus 2, China

Cited by 339 publications

References 6 publications

Integrated analyses of single-cell atlases reveal age, gender, and smoking status associations with cell type-specific expression of mediators of SARS-CoV-2 viral entry and highlights inflammatory programs in putative target cells

Integrated analyses of single-cell atlases reveal age, gender, and smoking status associations with cell type-specific expression of mediators of SARS-CoV-2 viral entry and highlights inflammatory programs in putative target cells

Effects of COVID-19 infection during pregnancy and neonatal prognosis: what is the evidence?

COVID‐19 in Pediatrics

Contact Info

Product

Resources

About