Lack of TNFα mRNA expression in cervical cancer is not associated with loss of heterozygosity at 6p21.3, inactivating mutations or promoter methylation

Human Sgo1 downregulation leads to chromosomal instability in colorectal cancer

“…We found a lower level of hSgo1 expression at the transcription and protein level in colorectal cancer tissue than in normal colorectal mucosa, suggesting a key role for hSgo1 in colorectal carcinogenesis. In the same way as in a previous study of another gene in which Kloth et al concluded that there was no association between the tumour necrosis factor α (TNFα) mRNA expression level and LOH at the TNFα locus,33 no association between LOH at the hSgo1 locus and hSgo1 mRNA expression level was found in our study, suggesting that hSgo1 mRNA expression is controlled by another factor, such as transcriptional or post-transcriptional activity, and not by LOH. Suzuki et al reported that a western blot analysis of human cell lines yielded proteolytic cleavage fragments as well as full-length hSgo1 protein 21.…”

Tumor Necrosis Factor α −308 G>A Polymorphisms and Cervical Cancer Risk

“…25 Some evidence has confirmed the absence of TNF-> expression in a substantial number of cervical tumors. 7 The mechanical analysis revealed that TNF-> might induce apoptosis in tumor cells in a similar way with the Fas ligand. 26 Moreover, studies have described that HPV-induced TNF-> expression was involved in the mediation of the upregulated expression of human leukocyte antigen class I antigen-processing and antigen-presentation pathway components 27 ; hence, the insufficient TNF-> expression may result in persistence of HPV16 infection and cervical cancer progression owing to insufficient antigen presentation to T cells.…”

“…6 Furthermore, the relationship between TNF-> levels and cervical cancer risk has been observed in previous studies. 7 Therefore, it is conceivable that the TNF-> j308 G9A polymorphism may be related with the cervical cancer risk. However, results of studies that examined the association of this single-nucleotide polymorphism with the risk of cervical cancer have been contradictive.…”

High‐Resolution Multiparameter DNA Flow Cytometry for the Detection and Sorting of Tumor and Stromal Subpopulations from Paraffin‐Embedded Tissues

“…This procedure allows the simultaneous immuno‐phenotypic identification of tumor stromal cells and carcinoma cells and labeling of DNA for ploidy measurements and sorting of tumor (sub)populations. The method has been successfully applied to study DNA ploidy and molecular genetic alterations in tumor cells derived from archival FFPE gastric (Carvalho et al, ), cervical (de Boer et al, ; Kloth et al, ), and colon cancer samples (Middeldorp et al, ), and has several advantages: The vimentin‐positive stromal cell fraction can be used as an internal DNA diploid reference (Qiu et al, ) for ploidy measurements of FFPE carcinomas. For example, this allows plain identification of tumor cells with a DNA index lower than 1.0 in FFPE samples without the aid of normal cells from another sample of the same patient. By using appropriate anti‐keratin antibodies, carcinoma cells can be readily identified, thereby improving S‐phase estimates especially in tumors with a DNA index close to 1.0.…”