Lack of Relationship Between Sialic Acid Content, Toxicity, and Lethality of Escherichia coli.

Relation of Structure to Function in Bacterial O‐antigens. Iv. Fractionation of the Components*†

“…These two procedures were shown to be the most effective solvents for a wide variety of different components from the bacterial cell walls. Colominic acid (Barry and Goebel, 1957), the different K antigens ((Drskov and coworkers, 1963), sialic acid-containing components (Forbes and Kuck, 1961), or colanic acid (Goebel, 1963) were all isolated from the water phase of the phenol-water extraction procedure. I t is ?Lethality was measured in 10 day old chick embryos by chorioallantoic inoculation.…”