Lack of mutagenicity, genotoxicity and developmental toxicity in safety assessment tests of Lactobacillus mali APS1

Lin, Yu‐Chun; Chen, Yung-Tsung; Chen, Ming‐Ju

doi:10.1371/journal.pone.0208881

Cited by 8 publications

(9 citation statements)

References 35 publications

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“…36 The results of our study showed that there were no genotoxic reactions in empirical animals at various maximum doses, which is consistent with the findings of various equivalent in vivo genotoxic studies on probiotics. 32,34,35 Furthermore, consistent with other studies, 32,[36][37][38] 28-day administration of the tested Lactobacillus strains in three doses does not cause any oral toxicity or mortality in animals.…”

Section: Discussionsupporting

confidence: 89%

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Safety evaluation of mutagenicity, genotoxicity, and cytotoxicity of Lactobacillus spp. isolates as probiotic candidates

Darbandi

Mirkalantari

Zadeh

et al. 2022

Clinical Laboratory Analysis

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Background: Probiotics promote a healthy balance of gut bacteria and have many beneficial effects on human digestive physiology. Although, few side effects of probiotics have been reported. This study aimed to assess the safety of five probiotic candidate Lactobacillus strains isolated from healthy individuals by examining mutagenicity, genotoxicity, and oral toxic effects. Methods: Five selected candidate probiotic (SCPs) strains were evaluated for genotoxicity (Ames test with Salmonella typhimurium), in vitro mammalian chromosome aberration test and an in vivo mouse micronucleus assay on peripheral blood of mice. To evaluate the oral dose toxicity, BALB/c mice models were treated repeatedly (2000, 1000, and 500 mg/kg body weight /day) for 28-days. Results:The Ames test performed for two S. typhimurium strains TA 98 and TA100 (both in the absence and in the presence of S-9 metabolic activation system) did not show an increase in reverse mutation because of exposure to the SCPs in any of the doses (5.0, 2.5, 1.25, 0.625, and 0.3125 mg/plate). There was no genotoxicity in the SCPs treatment in the vitro chromosome aberration assay with Chinese hamster ovary cells (CHO-K1). In addition, none of the tested strains increased the frequency of micronucleated reticulocytes in reticulocytes, the SCPs with the studied doses caused no substantial variation in the experimental groups compared to the negative control group (p > 0.05). SCPs were not acutely toxic when administered to male and female BALB/c mice by single gavage at (2000, 1000, and 500 mg/kg b.w/day) with no mortality or clinical signs, change in body weight or macroscopic abnormalities were observed in this dose range. Conclusion:As a result, SCPs did not induce mutagenic potential in vitro with bacterial reverse mutation, clastogenicity, and in vivo tests in the ranges of concentrations evaluated in our study.

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Section: Discussionsupporting

confidence: 89%

“…rhamnosus LCR177, B . adolescentis BA286, and Pediococcus acidilactici PA318, 31 Lactobacillus mali APS1 32 on worked with S . typhimurium strains TA97a, TA98, TA100, TA102, and TA1535 and also other LAB B .…”

Section: Discussionmentioning

confidence: 99%

Safety evaluation of mutagenicity, genotoxicity, and cytotoxicity of Lactobacillus spp. isolates as probiotic candidates

Darbandi

Mirkalantari

Zadeh

et al. 2022

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

“…The Salmonella reversion-based Salmonella /microsome test (Ames test) developed in 1973 by Bruce Ames ( Ames et al, 1973 ) is a widely used bioassay to determine mutagenic potential of compounds in toxicological risk assessment ( Zwart et al, 2018 ). Thus, Ames is the most widely used method for mutagenicity testing in probiotics, including L. plantarum , L. paracasei , B. adolescentis , and P. acidilactici ( Chiu et al, 2013 ; Tseng et al, 2015 ; Lin et al, 2018 ; Liao et al, 2019 ). Herein, our findings indicated that the tested strain, namely, K56, ET-22, and BL-99, were negative in Ames test, which indicated that the tested strains had no genetic mutagenicity.…”

Section: Discussionmentioning

confidence: 99%

Safety Evaluation of Bifidobacterium lactis BL-99 and Lacticaseibacillus paracasei K56 and ET-22 in vitro and in vivo

Wen

Liu

et al. 2021

Front. Microbiol.

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Probiotics have been reported to play a major role in maintaining the balance of microbiota in host. Consumption of food with probiotics has increased with consumer concerns regarding healthy diets and wellness. Correspondingly, safety evaluation of probiotics for human consumption has become increasingly important in food industry. Herein, we aimed to test the safety of Bifidobacterium lactis BL-99 and Lacticaseibacillus paracasei K56 and ET-22 strains in vitro and in vivo. In results, these strains were found to be negative for mucin degradation and platelet aggregation test. Additionally, the three strains were susceptible to eight antibiotics. In accordance with bacterial reversion mutation (Ames) assay, the tested strains had no genetic mutagenicity. Finally, it was confirmed that there were no dose-dependent mortality and toxicity throughout multidose oral toxicity tests in rats. Our findings demonstrated that B. lactis BL-99 and L. paracasei K56 and ET-22 can achieve the generally recognized as safe (GRAS) status as probiotics in the future.

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“…The bacterial reverse mutation assay, developed by Bruce Ames in 1973 [44], was performed for mutagenicity testing of probiotics such as L. rhamnosus, B. adolescentis, L. paracasei, L. mali, and P. acidilactici [45][46][47]. The genotoxicity was conducted by this assay with different doses of W. cibaria JW15 against four mutant S. typhimurium strains (TA98, TA100, TA1535, and TA1537) and a mutant E. coli strain (WP2uvrA), respectively.…”

Section: Bacterial Reverse Mutation Assaymentioning

confidence: 99%

Safety Evaluation of Weissella cibaria JW15 by Phenotypic and Genotypic Property Analysis

et al. 2021

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Weissella cibaria is one of the bacteria in charge of the initial fermentation of kimchi and has beneficial effects such as immune-modulating, antagonistic, and antioxidant activities. In our study, we aimed to estimate the safety of W. cibaria JW15 for the use of probiotics according to international standards based on phenotypic (antibiotic resistance, hemolysis, and toxic metabolite production) and genotypic analysis (virulence genes including antibiotic resistance genes). The results of the safety assessment on W. cibaria JW15 were as follows; (1) antibiotic resistance genes (ARGs) (kanamycin and vancomycin etc.) were intrinsic characteristics; (2) There were no acquired virulence genes including Cytolysin (cylA), aggregation substance (asa1), Hyaluronidase (hyl), and Gelatinase (gelE); (3) this strain also lacked β-hemolysis and the production of toxic metabolites (D-lactate and bile salt deconjugation). Consequently, W. cibaria JW15 is expected to be applied as a functional food ingredient in the food market.

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Lack of mutagenicity, genotoxicity and developmental toxicity in safety assessment tests of Lactobacillus mali APS1

Cited by 8 publications

References 35 publications

Safety evaluation of mutagenicity, genotoxicity, and cytotoxicity of Lactobacillus spp. isolates as probiotic candidates

Safety evaluation of mutagenicity, genotoxicity, and cytotoxicity of Lactobacillus spp. isolates as probiotic candidates

Safety Evaluation of Bifidobacterium lactis BL-99 and Lacticaseibacillus paracasei K56 and ET-22 in vitro and in vivo

Safety Evaluation of Weissella cibaria JW15 by Phenotypic and Genotypic Property Analysis

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