Lack of mutagenic effect by multi-walled functionalized carbon nanotubes in the somatic cells of Drosophila melanogaster

Machado, Nayane Moreira; Lopes, Jeyson Césary; Saturnino, Rosiane Soares; Fagan, Evandro Binotto; Nepomuceno, Júlio César

doi:10.1016/j.fct.2013.08.051

Cited by 20 publications

(14 citation statements)

References 26 publications

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“…However, in spite of numerous efforts, no consensus has been reached regarding the safety of CNTs, with some studies finding these materials to be genotoxic or carcinogenic 21 and others finding them to be safe. 22 It is anticipated that the meta-stability and water dispersibility of GO may offer better prospects for biological compatibility than CNTs. GO is reported to be an autodegrading material on the time scale of months, with an aqueous degradation pathway resulting in the formation of humic acid.…”

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confidence: 99%

In Vivo Compatibility of Graphene Oxide with Differing Oxidation States

Sydlik¹,

et al. 2015

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Graphene oxide (GO) is suggested to have great potential as a component of biomedical devices. Although this nanomaterial has been demonstrated to be cytocompatible in vitro, its compatibility in vivo in tissue sites relevant for biomedical device application is yet to be fully understood. Here, we evaluate the compatibility of GO with two different oxidation levels following implantation in subcutaneous and intraperitoneal tissue sites, which are of broad relevance for application to medical devices. We demonstrate GO to be moderately compatible in vivo in both tissue sites, with the inflammatory reaction in response to implantation consistent with a typical foreign body reaction. A reduction in the degree of GO oxidation results in faster immune cell infiltration, uptake, and clearance following both subcutaneous and peritoneal implantation. Future work toward surface modification or coating strategies could be useful to reduce the inflammatory response and improve compatibility of GO as a component of medical devices.

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confidence: 99%

In Vivo Compatibility of Graphene Oxide with Differing Oxidation States

Sydlik¹,

et al. 2015

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“…Adult D. melanogaster, when dusted, or larva, when fed with CNTs, have no increase in mortality compared to control groups 46 . In D. melanogaster somatic cells, functionalised CNTs demonstrate low cytotoxicity 47 . The soil arthropod, Folsomia candida, when exposed to CNTs show no detrimental effects on mortality or reproduction 48 .…”

Section: Discussionmentioning

confidence: 99%

Dendrimer-coated carbon nanotubes deliver dsRNA and increase the efficacy of gene knockdown in the red flour beetle Tribolium castaneum

Edwards

Christie

Masotti

et al. 2020

Sci Rep

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In this study, the use of dendrimer-coated carbon nanotubes (CNTs) as a delivery vehicle for dsRNA was assessed in Tribolium castaneum. Exposure to low dosages of polyamidoamine dendrimer carbon nanotubes (PAMAM-CNTs) did not affect T. castaneum larval mortality. Expression of key apoptotic factors, Dronc (Tc12580), Dredd (Tcn-like, Tc014026) and Buffy, (Tcinhib apop1), which can act as toxicity indicators, were not altered in T. castaneum larvae following injection of PAMAM-CNTs. The level of knockdown of two target genes, α-tubulin and mitochondrial RNA polymerase (mtpol), were significantly increased when larvae were injected with double-stranded RNA bound to CNTs (PAMAM-CNT-dsRNA), compared to those injected with target dsRNA alone. PAMAM-CNTs were visualised in cellular vacuoles and in the cell nucleus. Increase occurrence of a blistered wing phenotype was found in a subset of PAMAM-CNT-dsRNA αtub injected larvae, relative to the level seen in larvae injected with naked dsRnA αtub alone. These results suggest that the use of functionalised CNTs for dsRNA delivery could increase the efficacy of RNA interference in insect pest species. RNA interference (RNAi) is a promising tool for the control of insect pests. Introduction of exogenous doublestranded RNA (dsRNA) is effective at triggering gene knockdown and associated phenotypes in many problem pest species such as the Western Corn Rootworm 1 , the Colorado potato beetle 2 and the Varroa mite 3. New developments in dsRNA delivery methods to crop pests, such as dsRNA expression in transgenic plants 4 and foliar application 5 , offer the possibility of dsRNA-based insecticides 6. Despite this promise, there is notable variation in RNAi response between insect species 7 , with some, such as many lepidopterans 8 , unable to mount a strong RNAi response to dsRNA. Ingestion of dsRNA can be a valid route for delivery, yet many insects secrete enzymes in their gut that breakdown dsRNA before the latter can elicit an effect on target gene transcripts 9-11. When dsRNA enters into the cytoplasm, it is generally very effective in mounting an RNAi response 12,13. However, the route to the cytoplasm can act as a barrier to effective RNAi, with stability and uptake of dsRNA within the insect thought to be the significant limiting factor 10,11,14,15. Therefore, the protection of dsRNA from degradation coupled with an efficient cellular uptake is key to RNAi success. The development of delivery vehicles or carriers that enable these two processes is now necessary to fully realise the potential for dsRNA as an effective control measure. Combining dsRNA with other substances that act as efficient carriers, such as chitosan 16,17 , perfluorocarbon nanoparticles 18-20 or ribonuceloparticles 21 , can stabilise dsRNA and increase the chances of successful gene knockdown. Encapsulation of dsRNA within carbon quantum dots 22 and liposomes 23,24 has also shown some promise in increasing efficacy in more challenging insect species. In the last decade, carbon nanotubes (CNTs) have emerg...

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“…The wing-spot assay can detect a wide range of mutational events, as well as mitotic recombination, a relevant process related with carcinogenesis (Luo et al, 2000). In addition, this system assay has already been demonstrated to be an excellent tool to assess genotoxic effects of different NM, including silver and cobalt NP (Demir et al, 2011; Vales et al, 2012); titanium, zirconium and aluminium metal oxide NP (Demir et al, 2013); and multi-walled carbon nanotubes (de Andrade et al, 2014; Machado et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Genotoxic and oxidative stress potential of nanosized and bulk zinc oxide particles in Drosophila melanogaster

Carmona

Inostroza‐Blancheteau

Rubio

et al. 2016

Toxicol Ind Health

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Zinc oxide nanoparticles (ZnONP) are manufactured on a large scale and can be found in a variety of consumer products, such as sunscreens, lotions, paints and food additives. Few studies have been carried out on its genotoxic potential and related mechanisms in whole organisms. In the present study, the in vivo genotoxic activity of ZnONP and its bulk form was assayed using the wing-spot test and comet assay in Drosophila melanogaster Additionally, a lipid peroxidation analysis using the thiobarbituric acid assay was also performed. Results obtained with the wing-spot test showed a lack of genotoxic activity of both ZnO forms. However, when both particle sizes were tested in the comet assay using larvae haemocytes, a significant increase in DNA damage was observed for ZnONP treatments but only at the higher dose applied. In addition, the lipid peroxidation assay showed significant malondialdehyde (MDA) induction for both ZnO forms, but the induction of MDA for ZnONP was higher for the ZnO bulk, suggesting that the observed DNA strand breaks could be induced by mediated oxidative stress. The overall data suggest that the potential genotoxicity of ZnONP in Drosophila can be considered weak according to the lack of mutagenic and recombinogenic effects and the induction of primary DNA damage only at high toxic doses of ZnONP. This study is the first assessing the genotoxic and oxidative stress potential of nano and bulk ZnO particles in Drosophila.

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Lack of mutagenic effect by multi-walled functionalized carbon nanotubes in the somatic cells of Drosophila melanogaster

Cited by 20 publications

References 26 publications

In Vivo Compatibility of Graphene Oxide with Differing Oxidation States

In Vivo Compatibility of Graphene Oxide with Differing Oxidation States

Dendrimer-coated carbon nanotubes deliver dsRNA and increase the efficacy of gene knockdown in the red flour beetle Tribolium castaneum

Genotoxic and oxidative stress potential of nanosized and bulk zinc oxide particles in Drosophila melanogaster

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