<i>In Vivo</i> Compatibility of Graphene Oxide with Differing Oxidation States

Sydlik, Stefanie A.; Jhunjhunwala, Siddharth; Webber, Matthew J.; Anderson, Daniel G.; Langer, Robert

doi:10.1021/acsnano.5b01290

Cited by 201 publications

(194 citation statements)

References 52 publications

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“…29,30 Graphene oxide, especially at low concentrations, does not show obvious cytotoxic effects on the human-derived cell lines A549 and SH-SY5Y. 31,32 However, graphene oxide reduces glioblastoma cell viability and proliferation with increasing doses.…”

Section: Resultsmentioning

confidence: 99%

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Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion

Wierzbicki¹,

Jaworski²,

Kutwin³

et al. 2017

IJN

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The highly invasive nature of glioblastoma is one of the most significant problems regarding the treatment of this tumor. Diamond nanoparticles (ND), graphite nanoparticles (NG), and graphene oxide nanoplatelets (nGO) have been explored for their biomedical applications, especially for drug delivery. The objective of this research was to assess changes in the adhesion, migration, and invasiveness of two glioblastoma cell lines, U87 and U118, after ND, NG, and nGO treatment. All treatments affected the cell surface structure, adhesion-dependent EGFR/AKT/mTOR, and β-catenin signaling pathways, decreasing the migration and invasiveness of both glioblastoma cell lines. The examined nanoparticles did not show strong toxicity but effectively deregulated cell migration. ND was effectively taken up by cells, whereas nGO and NG strongly interacted with the cell surface. These results indicate that nanoparticles could be used in biomedical applications as a low toxicity active compound for glioblastoma treatment.

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Section: Resultsmentioning

confidence: 99%

“…Adhesion test was performed in four time points (15,30,60, and 120 min). Treatment with ND, NG, or nGO decreased adhesion of both glioma cell lines.…”

Section: Nanoparticles Decrease Adhesion and Migration Of Glioblastommentioning

confidence: 99%

Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion

Wierzbicki¹,

Jaworski²,

Kutwin³

et al. 2017

IJN

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show abstract

“…Nanomaterials have been shown to interact with cells of the innate immune system, while effects on the adaptive immune system occur, in most but not all cases, via the innate immune system [see 8, 28 for a review]. To give one recent example, GO was shown to trigger a typical ‘foreign body’ reaction in mice upon subcutaneous implantation, with recruitment of neutrophils, followed by monocytes; these cells secreted a variety of soluble mediators resulting in the establishment of an inflammatory microenvironment [29]. GO and CNTs have both been reported to act directly on macrophages and DCs ex vivo and in animal models [30–32].…”

Section: Biocompatibility Of Carbon-based Nanomaterialsmentioning

confidence: 99%

Biological interactions of carbon-based nanomaterials: From coronation to degradation

Bhattacharya

Mukherjee

Gallud

et al. 2016

Nanomedicine: Nanotechnology, Biology and Medicine

346

226

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Carbon-based nanomaterials including single- and multi-walled carbon nanotubes, graphene oxide, fullerenes and nanodiamonds are potential candidates for various applications in medicine such as drug delivery and imaging. However, the successful translation of nanomaterials for biomedical applications is predicated on a detailed understanding of the biological interactions of these materials. Indeed, the potential impact of the so-called bio-corona of proteins, lipids, and other biomolecules on the fate of nanomaterials in the body should not be ignored. Enzymatic degradation of carbon-based nanomaterials by immune-competent cells serves as a special case of bio-corona interactions with important implications for the medical use of such nanomaterials. In the present review, we highlight emerging biomedical applications of carbon-based nanomaterials. We discuss recent studies on nanomaterial ‘coronation’ and how this impacts on biodistribution and targeting along with studies on the enzymatic degradation of carbon-based nanomaterials, and the role of surface modification of nanomaterials for these biological interactions.

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“…37,38 However, the relationship between GO and autophagic flux in neuronal cells is not clear. We investigated this relationship using TEM and immunoblot analysis after cellular uptake of GO.…”

Section: Cellular Uptake Of Go Induces Accumulation Of Autophagosomesmentioning

confidence: 99%

Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments

Jeong

Lee

Jeong

et al. 2017

IJN

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Graphene oxide (GO) is a nanomaterial with newly developing biological applications. Autophagy is an intracellular degradation system that has been associated with the progression of neurodegenerative disorders. Although induction of autophagic flux by GO has been reported, the underlying signaling pathway in neurodegenerative disorders and how this is involved in neuroprotection remain obscure. We show that GO itself activates autophagic flux in neuronal cells and confers a neuroprotective effect against prion protein (PrP) (106-126)-mediated neurotoxicity. GO can be detected in SK-N-SH neuronal cells, where it triggers autophagic flux signaling. GO-induced autophagic flux prevented PrP (106-126)-induced neurotoxicity in SK-N-SH cells. Moreover, inactivation of autophagic flux blocked GO-induced neuroprotection against prion-mediated mitochondrial neurotoxicity. This is the first study to demonstrate that GO regulates autophagic flux in neuronal cells, and that activation of autophagic flux signals, induced by GO, plays a neuroprotective role against prion-mediated mitochondrial neurotoxicity. These results suggest that the nanomaterial GO may be used to activate autophagic flux and could be used in neuroprotective strategies for treatment of neurodegenerative disorders, including prion diseases.

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In Vivo Compatibility of Graphene Oxide with Differing Oxidation States

Cited by 201 publications

References 52 publications

Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion

Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion

Biological interactions of carbon-based nanomaterials: From coronation to degradation

Autophagic flux induced by graphene oxide has a neuroprotective effect against human prion protein fragments

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